General information for g7321.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Eukaryotic translation initiation factor 2 subunit 1.
Parent gene
- The parent gene g7321 is located on chr_2: 22941257..22942379 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7321 | g7321.t3 | TTS | g7321.t3 | 22940445 | 22940445 |
| chr_2 | g7321 | g7321.t3 | isoform | g7321.t3 | 22940724 | 22941978 |
| chr_2 | g7321 | g7321.t3 | exon | g7321.t3.exon1 | 22940724 | 22940909 |
| chr_2 | g7321 | g7321.t3 | cds | g7321.t3.CDS1 | 22940724 | 22940909 |
| chr_2 | g7321 | g7321.t3 | exon | g7321.t3.exon2 | 22940971 | 22941086 |
| chr_2 | g7321 | g7321.t3 | cds | g7321.t3.CDS2 | 22940971 | 22941086 |
| chr_2 | g7321 | g7321.t3 | exon | g7321.t3.exon3 | 22941156 | 22941617 |
| chr_2 | g7321 | g7321.t3 | cds | g7321.t3.CDS3 | 22941156 | 22941617 |
| chr_2 | g7321 | g7321.t3 | exon | g7321.t3.exon4 | 22941676 | 22941978 |
| chr_2 | g7321 | g7321.t3 | cds | g7321.t3.CDS4 | 22941676 | 22941856 |
| chr_2 | g7321 | g7321.t3 | TSS | g7321.t3 | 22942380 | 22942380 |
Sequences
>g7321.t3 Gene=g7321 Length=1067
ATGGTTTTAACGTGTCGCTTTTATAAGCAAAAATACCCGGAAGTAAGTGTCTTTAAATTG
TTATTAAAAATATCTATTTAACAATTTAATAAAATATAATTTTTCAGGTGGAAGATGTTG
TGATGGTAAATGTGAGATCAATAGCTGAAATGGGTGCCTACGTTCATTTATTAGAATACA
ATAATATTGAGGGTATGATTCTACTATCAGAATTATCAAGAAGACGTATTCGTTCCATCA
ATAAGCTCATTCGAGTTGGAAAAACTGAACCAGTTGTTGTTATTCGTGTTGATAAAGAAA
AGGGGTATATTGATTTATCAAAACGTCGTGTGTCGCCTGAGGATGTAGAAAAATGTACAG
AACGTTTTAGTAAAGCTAAAGCAGTGAGCTCAATTTTGCGGCATGTCGCAGAACTTCTCA
AATACGATACTGATGAGCAGTTAGAAGATTTATATCAAAAGACTGCATGGCATTTCGAGG
AGAAGCATAAAAATAAAACAGCGTCCTATGACATTTTTAAGCAAGCAGTTACGGAACCAT
CGCTATTGGATGAATGCAATCTAGACGAAAATACAAAGAATGTGCTTTTGACGAATATCA
AAAGGAAATTAACTTCACAAGCAATGAAAATTCGTGCAGACATTGAATGTGCATGTTACG
GATATGAGGGCATTGATGCTGTTAAACAAGCTTTGCGTGCTGGTCTTGAAACATCAACTG
AAGATATTCCAATTAAAATCAATTTGATTGCGCCGCCCATTTATGTCATAACGACATCGA
CTCCTGAAAAAAATGATGGTCTTAAGGCTCTTGAAAATGCCATAGATAAAATCAAGTCTA
CAATTGAGAAGCTTGACGGACATTTTGCTATTCAAATGGCGCCTAAAGTTGTTACTGCAA
TTGATGAAGCTGATTTAGCAAGACGAATGGAACGCGCTGAAATGGAAAATGCAGAAGTTT
CAGGAGATGAAGATGAAAGTGAAGAGGAAGAAGGTATGAAATTCGATGCTGATGAGAATG
ATACAGGTGACAAAAATTCAGACAAATCAGGTTCTGACGATGAATAA
>g7321.t3 Gene=g7321 Length=314
MVNVRSIAEMGAYVHLLEYNNIEGMILLSELSRRRIRSINKLIRVGKTEPVVVIRVDKEK
GYIDLSKRRVSPEDVEKCTERFSKAKAVSSILRHVAELLKYDTDEQLEDLYQKTAWHFEE
KHKNKTASYDIFKQAVTEPSLLDECNLDENTKNVLLTNIKRKLTSQAMKIRADIECACYG
YEGIDAVKQALRAGLETSTEDIPIKINLIAPPIYVITTSTPEKNDGLKALENAIDKIKST
IEKLDGHFAIQMAPKVVTAIDEADLARRMERAEMENAEVSGDEDESEEEEGMKFDADEND
TGDKNSDKSGSDDE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g7321.t3 | CDD | cd04452 | S1_IF2_alpha | 1 | 69 | 3.7646E-39 |
| 11 | g7321.t3 | Coils | Coil | Coil | 227 | 247 | - |
| 10 | g7321.t3 | Coils | Coil | Coil | 269 | 289 | - |
| 8 | g7321.t3 | Gene3D | G3DSA:2.40.50.140 | - | 1 | 70 | 2.7E-30 |
| 9 | g7321.t3 | Gene3D | G3DSA:1.10.150.190 | Translation initiation factor 2; subunit 1; domain 2 | 71 | 161 | 9.9E-36 |
| 7 | g7321.t3 | Gene3D | G3DSA:3.30.70.1130 | EIF_2_alpha | 167 | 312 | 2.7E-44 |
| 16 | g7321.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 270 | 314 | - |
| 15 | g7321.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 277 | 296 | - |
| 14 | g7321.t3 | MobiDBLite | mobidb-lite | consensus disorder prediction | 297 | 314 | - |
| 3 | g7321.t3 | PANTHER | PTHR10602 | EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1 | 1 | 292 | 1.9E-125 |
| 1 | g7321.t3 | Pfam | PF00575 | S1 RNA binding domain | 3 | 68 | 2.5E-15 |
| 2 | g7321.t3 | Pfam | PF07541 | Eukaryotic translation initiation factor 2 alpha subunit | 110 | 223 | 6.8E-36 |
| 17 | g7321.t3 | ProSiteProfiles | PS50126 | S1 domain profile. | 1 | 68 | 16.624 |
| 13 | g7321.t3 | SMART | SM00316 | S1_6 | 1 | 68 | 5.8E-8 |
| 6 | g7321.t3 | SUPERFAMILY | SSF50249 | Nucleic acid-binding proteins | 3 | 73 | 1.63E-15 |
| 5 | g7321.t3 | SUPERFAMILY | SSF116742 | eIF2alpha middle domain-like | 70 | 162 | 6.93E-33 |
| 4 | g7321.t3 | SUPERFAMILY | SSF110993 | eIF-2-alpha, C-terminal domain | 166 | 282 | 9.29E-41 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003743 | translation initiation factor activity | MF |
| GO:0003723 | RNA binding | MF |
| GO:0003676 | nucleic acid binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
