General information for g7357.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Signal transducing adapter molecule 1.
Parent gene
- The parent gene g7357 is located on chr_2: 23365161..23367670 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7357 | g7357.t1 | TSS | g7357.t1 | 23365030 | 23365030 |
| chr_2 | g7357 | g7357.t1 | isoform | g7357.t1 | 23365161 | 23367670 |
| chr_2 | g7357 | g7357.t1 | exon | g7357.t1.exon1 | 23365161 | 23365206 |
| chr_2 | g7357 | g7357.t1 | cds | g7357.t1.CDS1 | 23365161 | 23365206 |
| chr_2 | g7357 | g7357.t1 | exon | g7357.t1.exon2 | 23365618 | 23365778 |
| chr_2 | g7357 | g7357.t1 | cds | g7357.t1.CDS2 | 23365618 | 23365778 |
| chr_2 | g7357 | g7357.t1 | exon | g7357.t1.exon3 | 23365836 | 23365952 |
| chr_2 | g7357 | g7357.t1 | cds | g7357.t1.CDS3 | 23365836 | 23365952 |
| chr_2 | g7357 | g7357.t1 | exon | g7357.t1.exon4 | 23366010 | 23366444 |
| chr_2 | g7357 | g7357.t1 | cds | g7357.t1.CDS4 | 23366010 | 23366444 |
| chr_2 | g7357 | g7357.t1 | exon | g7357.t1.exon5 | 23366501 | 23367425 |
| chr_2 | g7357 | g7357.t1 | cds | g7357.t1.CDS5 | 23366501 | 23367425 |
| chr_2 | g7357 | g7357.t1 | exon | g7357.t1.exon6 | 23367483 | 23367670 |
| chr_2 | g7357 | g7357.t1 | cds | g7357.t1.CDS6 | 23367483 | 23367670 |
| chr_2 | g7357 | g7357.t1 | TTS | g7357.t1 | 23367967 | 23367967 |
Sequences
>g7357.t1 Gene=g7357 Length=1872
ATGGGATTATTTGGGAACTCACAATCGCCATTGGAAAGTGAAATAGAGAAAGCTACAAGT
GAAAACAACACGACAGAAAAATGGTCGCTGATAATGGACATTTGTGATAAAATTGGTAGC
AGTCAAGTAAATGCGAAGGAAAGTTTGAAGATAATAATGAAACGCCTTAATCATGCGGAT
CCCCATGTTGTGATGCAAGCAATCACTCTTTTGGATGCCTGCGTAAATAATTGTGGAAAA
AATTATCATCTCGAAATTGCATCAAGAGAATTTGAACTTGAGTTCAAACGGCTTGTTAAC
AAAAGTGAACCACAAGTTGCGAATAAATTAAAATTATCACTTAAAAAGTGGGCAGAAGGA
GATTTCAAGTCAGATCCACAATTAAATTTAATTCCATCACTTTATAAAAAGATGAAAGCA
GAAGGACATGATTTTAGCGAACCACAAGCGACAGTTAAAAGAGAAGTAGAAAAGATTAGT
GATCCGAATGTAGTGTCTAGTCAGCAAGAAGAAGATGATATAGCAAAGGCCATTGAACTT
TCTCTCAAGGACGCAAATACGCCGAAAAAGAGTTCAATCGCATCAGCGGTTGCATCTTCA
TTATATCCTTCAATGAATTCTTTAGTAAATAATAATACTTCGAGTGCATCTGCATCAATT
GCAGCCCCAATACAAGAGCCAAGAAAAGTTCGTGCTCTTTATGATTTTGAAGCGGCAGAA
GACAACGAATTGACATTTAAAGCTGGTGAAGTTATAATGGTATTGGAAGAATCAGATCCA
AATTGGTGGAGAGGAACAAATCATAGAGGTGAAGGTTTTTTTCCTGCTAATTTTGTCACA
GCTGATTTAAGTGCAGAACCAGAAGATTTTTACAAAAAGGAAACAAGAAAGAGTAATAAA
TCAAATGACATCACTCATGAAGAACGAAAACCTGAACAGCCGGTTGAGATTAACGAAGAA
GCTATTGATCGTTTATTATTTTTGCTACATGAAGCAGATCCTGAAGATCCATCTCAAGAT
AGTGATGAAATGTTGAGATTAGAGCATTTCGTAACTCAAATGGGTCCCCTTATTGATTCA
GAATTGGAACGAGTCGATAGAAAGCATGCTCAGTTAACACAATTAAGCAGTGATTTAATT
GACACAATTAATCTTTATCATAGTCTTATGCGTGATACTGAGAAGGCACCAATGCGAATG
CTTAATAATTATCCTCCGCCAGGATTAGCAGGAAGACAATTTTATCCACCAAACAGTAAT
CCATATGGAATGCACATGGGAATGCCTGATCAAATGTCTGTTGCTTCTGCTTTTCCTTCA
CATATGACGCCTGAAAATTTTCATTTAATGCATTCGCAAGCAAATATGCATCAACCAACA
TCAATTCTTCAAAATTCTTCATTACCACCTCAATCAGAACATTCAATGATGCAACAACAA
TTTCCACAACAGCCTCATGTTTCTGCTGCTACTAATATGAATATGGGACAAATGCAAATG
CCTCCACCATCAGGACTTTATCAGAACAATGTTATGGGACAAATGTCACAACAACAGCCT
CAAGATCCTAATAGAATTCCAAATCAAGATATGGGTCAAAATCAAATTCCACAACAACAA
CAACATCAGCAACCTACTCAACTATCAGGAACAACATTCCCACAACAACAAAATCATAAT
TTAGCTCAAAACTTTTCATTACCGATGCAACAACCACCACATAACATGATGGCACCAAAT
TATTCACCAATGTCCACCAATCAAATGATGCAACAGACGAATCATCAACCAATGCAAATT
AATGAAATGATGGCTAATATGCAAATTCATTCAATGCCAAGCGTTCCACCAATGAGCTAT
CCTCCACAGTAA
>g7357.t1 Gene=g7357 Length=623
MGLFGNSQSPLESEIEKATSENNTTEKWSLIMDICDKIGSSQVNAKESLKIIMKRLNHAD
PHVVMQAITLLDACVNNCGKNYHLEIASREFELEFKRLVNKSEPQVANKLKLSLKKWAEG
DFKSDPQLNLIPSLYKKMKAEGHDFSEPQATVKREVEKISDPNVVSSQQEEDDIAKAIEL
SLKDANTPKKSSIASAVASSLYPSMNSLVNNNTSSASASIAAPIQEPRKVRALYDFEAAE
DNELTFKAGEVIMVLEESDPNWWRGTNHRGEGFFPANFVTADLSAEPEDFYKKETRKSNK
SNDITHEERKPEQPVEINEEAIDRLLFLLHEADPEDPSQDSDEMLRLEHFVTQMGPLIDS
ELERVDRKHAQLTQLSSDLIDTINLYHSLMRDTEKAPMRMLNNYPPPGLAGRQFYPPNSN
PYGMHMGMPDQMSVASAFPSHMTPENFHLMHSQANMHQPTSILQNSSLPPQSEHSMMQQQ
FPQQPHVSAATNMNMGQMQMPPPSGLYQNNVMGQMSQQQPQDPNRIPNQDMGQNQIPQQQ
QHQQPTQLSGTTFPQQQNHNLAQNFSLPMQQPPHNMMAPNYSPMSTNQMMQQTNHQPMQI
NEMMANMQIHSMPSVPPMSYPPQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g7357.t1 | CDD | cd03568 | VHS_STAM | 12 | 142 | 1.90034E-75 |
| 13 | g7357.t1 | CDD | cd11820 | SH3_STAM | 228 | 281 | 2.18757E-34 |
| 10 | g7357.t1 | Gene3D | G3DSA:1.25.40.90 | - | 4 | 186 | 9.7E-47 |
| 11 | g7357.t1 | Gene3D | G3DSA:2.30.30.40 | SH3 Domains | 213 | 295 | 1.9E-23 |
| 17 | g7357.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 20 | - |
| 20 | g7357.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 290 | 317 | - |
| 18 | g7357.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 466 | 486 | - |
| 19 | g7357.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 532 | 551 | - |
| 3 | g7357.t1 | PANTHER | PTHR45929 | JAK PATHWAY SIGNAL TRANSDUCTION ADAPTOR MOLECULE | 1 | 520 | 3.0E-148 |
| 7 | g7357.t1 | PRINTS | PR00452 | SH3 domain signature | 228 | 238 | 1.5E-10 |
| 6 | g7357.t1 | PRINTS | PR00452 | SH3 domain signature | 242 | 257 | 1.5E-10 |
| 5 | g7357.t1 | PRINTS | PR00452 | SH3 domain signature | 259 | 268 | 1.5E-10 |
| 4 | g7357.t1 | PRINTS | PR00452 | SH3 domain signature | 270 | 282 | 1.5E-10 |
| 2 | g7357.t1 | Pfam | PF00790 | VHS domain | 7 | 142 | 8.5E-38 |
| 1 | g7357.t1 | Pfam | PF00018 | SH3 domain | 231 | 276 | 4.3E-17 |
| 21 | g7357.t1 | ProSiteProfiles | PS50179 | VHS domain profile. | 18 | 146 | 38.775 |
| 22 | g7357.t1 | ProSiteProfiles | PS50330 | Ubiquitin-interacting motif (UIM) domain profile. | 169 | 188 | 10.243 |
| 23 | g7357.t1 | ProSiteProfiles | PS50002 | Src homology 3 (SH3) domain profile. | 225 | 284 | 28.228 |
| 15 | g7357.t1 | SMART | SM00288 | VHS_2 | 11 | 142 | 3.1E-49 |
| 16 | g7357.t1 | SMART | SM00726 | uim | 169 | 188 | 0.0036 |
| 14 | g7357.t1 | SMART | SM00326 | SH3_2 | 228 | 283 | 8.3E-23 |
| 9 | g7357.t1 | SUPERFAMILY | SSF48464 | ENTH/VHS domain | 9 | 146 | 2.36E-40 |
| 8 | g7357.t1 | SUPERFAMILY | SSF50044 | SH3-domain | 206 | 291 | 1.58E-22 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006886 | intracellular protein transport | BP |
| GO:0005515 | protein binding | MF |
KEGG
Orthology
- This transcript has been annotated with K04705 (signal transducing adaptor molecule)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
