General information for g7474.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Lethal(2)neighbour of tid protein.
Parent gene
- The parent gene g7474 is located on chr_2: 23915614..23916603 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7474 | g7474.t1 | TTS | g7474.t1 | 23913962 | 23913962 |
| chr_2 | g7474 | g7474.t1 | isoform | g7474.t1 | 23914019 | 23915614 |
| chr_2 | g7474 | g7474.t1 | exon | g7474.t1.exon1 | 23914019 | 23915259 |
| chr_2 | g7474 | g7474.t1 | cds | g7474.t1.CDS1 | 23914019 | 23915259 |
| chr_2 | g7474 | g7474.t1 | exon | g7474.t1.exon2 | 23915353 | 23915614 |
| chr_2 | g7474 | g7474.t1 | cds | g7474.t1.CDS2 | 23915353 | 23915614 |
| chr_2 | g7474 | g7474.t1 | TSS | g7474.t1 | 23915689 | 23915689 |
Sequences
>g7474.t1 Gene=g7474 Length=1503
ATGGCACCAAAAAATAAAAAGTCTGAAAAGAAGGGGTTGCTGCAGACATTTTACACAAAA
TATTTTACAATAGATTTTGCCAAATCACTTATATTTAATCCAGCTCAATTATCTCTTTTG
ACTTATTTTATTTTGACTGTTGAGTTGGCTCTAAATATATTTATTGTCTTTAGAGTAAGA
TATACTGAGATTGATTGGATTGCTTATATGCAAGAATGTGAAGGATTTATAAATGGAACA
TTAGATTATGCTCAATTGAAAGGCGATACTGGGCCACTCGTGTATCCAAGTTTCTTCGTT
TACATTTATTCATTGCTTTATTTTATTACATCTCGAGGAAGTAACATAAGATTAGCGCAA
TTCATTTTTATTGCAATTTATTTGCTGCAACTTTGGCTTGTTCTTCGCCTCTACTCAAAA
ACTAGAAAAGTTCCTCCATATATCATCATCATCAGCATTTTTACATCATATCGCATTCAT
TCTATTTATTCACTGAGACTTTTCAATGATCCTGTGGCGATTTTATTTCTTTATATTGCA
CTCAATCTCTTTATGGATCGAAAATGGAGTTCAGGAAGTGTGATTTTTAGTTTGGCTGTG
GGAATTAAAATGAATATTTTACTGTTTGCGCCTGCAATTTTAATGCTATATCTCGCATGC
TTGGGATTTTTAAGAACAATTTATCAGCTCTCCATTTGTGCTTCTGTCCAATTGCTCATT
GGTGCACCTTTTTTGTTGACTCATCCGATCTCATATATCAAAGGAAGTTTCGATTTAGGT
CGTGTTTTTGAGCACAAATGGACTGTAAATTATAGATTTTTAGACCGTGAAACATTTGAA
AATTCGACGTTTCACGTTGCTCTTTTGATTTTGCATCTTGTACTGCTTGTAGTTGTCACT
AAACCATGCTATCTTTTCTTCAAAAACTATGCACGCTTAAGAACTTTACAAGCTCAATTT
GAGCCTCAAATTGCTGCTGAAAACAGAGAAATAGAACAGGAGATTCAACAAAAAATGAAA
AAATTGAAGAAGAAAGTTAATGATAAAGAAGAACAGTTATCAACAGAACAAAAAAATTTC
TTGAAATCTTTTGAAAAAGGATTGAAAAATCAATTTGGTGAACAAAATCAACCAAAACCT
GAGCCAAAGGATGAAGATGATCCGAAAAAAGTTGAAATACATTTTGATCAATGTGTTCAA
TTGGCAATTTTACCAATTTTCCTTATCAACTTTATTGGAATCGTTTGTGCAAGATCACTT
CATTATCAATTTTATGTATGGTATTTTCATTCATTGCCATATCTCTCATGGTTCACAGAC
TATCATACAAGTTTCAAAATTCTTATCCTATTTTTGATCGAATTTTGCTGGAATCAATAT
CCTTCAACAGTCTTTTCAAGTGTTTTATTGCATGCATGTCATACAATTTTATTATTTGGA
GTTATTCGAAAAATTTATAAAGAAGTTGCAATGGCTAAGCAAGCATCAAGCAAACAAAAA
TGA
>g7474.t1 Gene=g7474 Length=500
MAPKNKKSEKKGLLQTFYTKYFTIDFAKSLIFNPAQLSLLTYFILTVELALNIFIVFRVR
YTEIDWIAYMQECEGFINGTLDYAQLKGDTGPLVYPSFFVYIYSLLYFITSRGSNIRLAQ
FIFIAIYLLQLWLVLRLYSKTRKVPPYIIIISIFTSYRIHSIYSLRLFNDPVAILFLYIA
LNLFMDRKWSSGSVIFSLAVGIKMNILLFAPAILMLYLACLGFLRTIYQLSICASVQLLI
GAPFLLTHPISYIKGSFDLGRVFEHKWTVNYRFLDRETFENSTFHVALLILHLVLLVVVT
KPCYLFFKNYARLRTLQAQFEPQIAAENREIEQEIQQKMKKLKKKVNDKEEQLSTEQKNF
LKSFEKGLKNQFGEQNQPKPEPKDEDDPKKVEIHFDQCVQLAILPIFLINFIGIVCARSL
HYQFYVWYFHSLPYLSWFTDYHTSFKILILFLIEFCWNQYPSTVFSSVLLHACHTILLFG
VIRKIYKEVAMAKQASSKQK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g7474.t1 | Coils | Coil | Coil | 325 | 359 | - |
| 2 | g7474.t1 | PANTHER | PTHR12646 | NOT56 - RELATED | 24 | 497 | 4.4E-146 |
| 1 | g7474.t1 | Pfam | PF05208 | ALG3 protein | 43 | 467 | 4.8E-142 |
| 16 | g7474.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 36 | - |
| 28 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 37 | 57 | - |
| 22 | g7474.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 58 | 92 | - |
| 27 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 93 | 109 | - |
| 14 | g7474.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 110 | 120 | - |
| 34 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 121 | 138 | - |
| 20 | g7474.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 139 | 143 | - |
| 32 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 144 | 160 | - |
| 18 | g7474.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 161 | 166 | - |
| 26 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 167 | 184 | - |
| 21 | g7474.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 185 | 195 | - |
| 25 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 196 | 220 | - |
| 13 | g7474.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 221 | 226 | - |
| 29 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 227 | 246 | - |
| 24 | g7474.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 247 | 283 | - |
| 33 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 284 | 307 | - |
| 15 | g7474.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 308 | 397 | - |
| 30 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 398 | 421 | - |
| 19 | g7474.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 422 | 440 | - |
| 31 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 441 | 457 | - |
| 17 | g7474.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 458 | 463 | - |
| 35 | g7474.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 464 | 482 | - |
| 23 | g7474.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 483 | 500 | - |
| 11 | g7474.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 35 | 57 | - |
| 3 | g7474.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 93 | 111 | - |
| 9 | g7474.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 116 | 138 | - |
| 4 | g7474.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 159 | 181 | - |
| 7 | g7474.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 196 | 218 | - |
| 8 | g7474.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 231 | 253 | - |
| 10 | g7474.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 285 | 307 | - |
| 5 | g7474.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 399 | 421 | - |
| 6 | g7474.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 460 | 482 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0000030 | mannosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K03845 (alpha-1,3-mannosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
