General information for g7477.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Sestrin-like protein.
Parent gene
- The parent gene g7477 is located on chr_2: 23934529..23935422 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7477 | g7477.t1 | isoform | g7477.t1 | 23931369 | 23934881 |
| chr_2 | g7477 | g7477.t1 | exon | g7477.t1.exon1 | 23931369 | 23931419 |
| chr_2 | g7477 | g7477.t1 | cds | g7477.t1.CDS1 | 23931369 | 23931419 |
| chr_2 | g7477 | g7477.t1 | exon | g7477.t1.exon2 | 23933464 | 23933529 |
| chr_2 | g7477 | g7477.t1 | cds | g7477.t1.CDS2 | 23933464 | 23933529 |
| chr_2 | g7477 | g7477.t1 | exon | g7477.t1.exon3 | 23933618 | 23933849 |
| chr_2 | g7477 | g7477.t1 | cds | g7477.t1.CDS3 | 23933618 | 23933849 |
| chr_2 | g7477 | g7477.t1 | exon | g7477.t1.exon4 | 23933972 | 23934240 |
| chr_2 | g7477 | g7477.t1 | cds | g7477.t1.CDS4 | 23933972 | 23934240 |
| chr_2 | g7477 | g7477.t1 | exon | g7477.t1.exon5 | 23934316 | 23934424 |
| chr_2 | g7477 | g7477.t1 | cds | g7477.t1.CDS5 | 23934316 | 23934424 |
| chr_2 | g7477 | g7477.t1 | exon | g7477.t1.exon6 | 23934529 | 23934881 |
| chr_2 | g7477 | g7477.t1 | cds | g7477.t1.CDS6 | 23934529 | 23934881 |
| chr_2 | g7477 | g7477.t1 | TTS | g7477.t1 | 23935430 | 23935430 |
| chr_2 | g7477 | g7477.t1 | TSS | g7477.t1 | NA | NA |
Sequences
>g7477.t1 Gene=g7477 Length=1080
ATGGAGATAGAGTACTTTCCAGAACTTGAAATGGGACAGATTAATCATGATTACTATTTT
AATAATAACAAGCCTTGGATAATGGAAAATGTTGATGTTTATGATCATGTCACAAAACAA
CACGTCATTAATTTGGCCATAATTGCAGCAGCTCGCAACAAATGCAACTATCTCGTGAGT
CTGCATGAAAAAGAGTTCTTGGCTGAAGGAGGTGATACAAAATGGCTAAATGGACTCGAA
TTTATTCCACAAAAGTTGCGTGCCATTTACGACATCAATAAAATTCTTGCTCATCGTCCA
TGGCTCTTAACCAAAGAACACATTGAGCGTTTGACGAAGGGCACAAATAAATCACCAACA
ATCGAACCAGAACCAGAAATTAAACCAAGTACTACGCCACCCTTCATTCAACGTCTTAAA
CTTGAACAGCAACGTATGCATCAAATGATGAAGGAGCAGGAAGAGCAAAGTAAAAAAGTT
CTTCAACAAAACAACTCAAATGGAACTTCAAGTGACAACATTGAGATGCTTATGAAACGC
ATGCAAGATCTCTCAGAAAAAAAGAAAGAATGCAGTGAAACTGAGCTGAGTAATCGTTTT
AAAAATGTTGAGCTTCAGGCTGCACAATTGACAAAATCAACAGAATCATTGAATGCAGTT
GAAGTTCCTCAATCGATAAGTCATTATGTAGAAGATCCAACTTATGCATATCAAGATTTT
GCTAGACATGATAAATTCCGTGTCGCTTACAACTTAACTTATAAGACACTCGCTGGTCGT
CAAAATGTCGACACATCGAAATTTCGTCGTGCAATATGGAATTATATTCAGTGCATCTAT
GGAATCAGACATGATGATTATGACTATGGCGAAATCAATCAACTTCTTGATCGTACTCTC
AATTCATTCATCAAAACAGCTTGTTGCTTCCCAGAGCGAATAACAAAGCAAGATTATGAC
AATGTTCTCGTGGAATTACTTGACAGTGAAAAAGTTCACGTTAATTTGATGATAATGGAG
GCAAAAAATCAAGCGATATTGCTTTATGCATTGAGAGAAATAATGCGCTATATGCTATGA
>g7477.t1 Gene=g7477 Length=359
MEIEYFPELEMGQINHDYYFNNNKPWIMENVDVYDHVTKQHVINLAIIAAARNKCNYLVS
LHEKEFLAEGGDTKWLNGLEFIPQKLRAIYDINKILAHRPWLLTKEHIERLTKGTNKSPT
IEPEPEIKPSTTPPFIQRLKLEQQRMHQMMKEQEEQSKKVLQQNNSNGTSSDNIEMLMKR
MQDLSEKKKECSETELSNRFKNVELQAAQLTKSTESLNAVEVPQSISHYVEDPTYAYQDF
ARHDKFRVAYNLTYKTLAGRQNVDTSKFRRAIWNYIQCIYGIRHDDYDYGEINQLLDRTL
NSFIKTACCFPERITKQDYDNVLVELLDSEKVHVNLMIMEAKNQAILLYALREIMRYML
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g7477.t1 | Coils | Coil | Coil | 136 | 156 | - |
| 9 | g7477.t1 | Coils | Coil | Coil | 174 | 194 | - |
| 7 | g7477.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 115 | 171 | - |
| 8 | g7477.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 144 | 158 | - |
| 5 | g7477.t1 | PANTHER | PTHR12474 | P53 REGULATED PA26 NUCLEAR PROTEIN SESTRIN | 45 | 116 | 8.1E-92 |
| 3 | g7477.t1 | PANTHER | PTHR12474 | P53 REGULATED PA26 NUCLEAR PROTEIN SESTRIN | 153 | 243 | 8.1E-92 |
| 4 | g7477.t1 | PANTHER | PTHR12474 | P53 REGULATED PA26 NUCLEAR PROTEIN SESTRIN | 243 | 358 | 8.1E-92 |
| 1 | g7477.t1 | Pfam | PF04636 | PA26 p53-induced protein (sestrin) | 45 | 117 | 1.3E-33 |
| 2 | g7477.t1 | Pfam | PF04636 | PA26 p53-induced protein (sestrin) | 244 | 358 | 1.5E-61 |
| 6 | g7477.t1 | SUPERFAMILY | SSF69118 | AhpD-like | 45 | 112 | 8.15E-11 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005634 | nucleus | CC |
| GO:1901031 | regulation of response to reactive oxygen species | BP |
KEGG
Orthology
- This transcript has been annotated with K10141 (sestrin 1/3)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
