General information for g7477.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Sestrin-like protein.
Parent gene
- The parent gene g7477 is located on chr_2: 23934529..23935422 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7477 | g7477.t5 | TSS | g7477.t5 | 23929439 | 23929439 |
| chr_2 | g7477 | g7477.t5 | isoform | g7477.t5 | 23929490 | 23935422 |
| chr_2 | g7477 | g7477.t5 | exon | g7477.t5.exon1 | 23929490 | 23929745 |
| chr_2 | g7477 | g7477.t5 | exon | g7477.t5.exon2 | 23931313 | 23931419 |
| chr_2 | g7477 | g7477.t5 | exon | g7477.t5.exon3 | 23933464 | 23934240 |
| chr_2 | g7477 | g7477.t5 | cds | g7477.t5.CDS1 | 23934067 | 23934240 |
| chr_2 | g7477 | g7477.t5 | exon | g7477.t5.exon4 | 23934316 | 23935422 |
| chr_2 | g7477 | g7477.t5 | cds | g7477.t5.CDS2 | 23934316 | 23934528 |
| chr_2 | g7477 | g7477.t5 | TTS | g7477.t5 | 23935430 | 23935430 |
Sequences
>g7477.t5 Gene=g7477 Length=2247
TTGAAATAAATAAAAAAAAACTTTATTGACGAGTTAAATAAATTTTAATAGACGACGAAG
GTTTCTAATATAACGCAAATTGCGGAAGAAAAAAATAATACAACTAGTGAGTGAGAAATA
AAAAAAATAAATTGAAGGATAATAATTTTCTAATAACGTTAAAAGTGTCATAAATTTATT
TTTGTGTTCGTTTATAAAAATTCTTATTTGCAACTTTATGAAAGAGACAAAATTTTAATT
AAAAATATTCAGTGAGCAAGAAGGGAAGAGTGAAAGAAGAAAGCCAAGTCAAAGACAGTA
AATTACAGCGCGATGGAGATAGAGTACTTTCCAGAACTTGAAATGGGACAGATTAATCAT
GATTACTATTTTAATAATAACAAGCCTTGGATAATGGAAAATGTTGATGTTTATGATCAT
GTCACAAAAGTAATTCGTTATCATCCGAAATACTTTGAACATTACATCAAAACACACAGA
TATTTGATGTATGATGATGGACCATTGCCATTCACAGCAACACGTCATTAATTTGGCCAT
AATTGCAGCAGCTCGCAACAAATGCAACTATCTCGTGAGTCTGCATGAAAAAGAGTTCTT
GGCTGAAGGAGGTGATACAAAATGGCTAAATGGACTCGAATTTATTCCACAAAAGTTGCG
TGCCATTTACGACATCAATAAAATTCTTGCTCATCGTCCATGGCTCTTAACCAAAGAACA
CATTGAGCGTTTGACGAAGGGCACAAATAGTTGGTCTTTGGCAGAAGTTGTTCATGCAAT
CGTCATTCTGGCACACTTTCATTCACTCTCATCTTTCGTATTCTCATGTGGTCTCAATCA
GGAATTGGATGCTAGTCTTAAAATGAACCAGAATCACCAACAATCGAACCAGAACCAGAA
ATTAAACCAAGTACTACGCCACCCTTCATTCAACGTCTTAAACTTGAACAGCAACGTATG
CATCAAATGATGAAGGAGCAGGAAGAGCAAAGTAAAAAAGTTCTTCAACAAAACAACTCA
AATGGAACTTCAAGTGACAACATTGAGATGCTTATGAAACGCATGCAAGATCTCTCAGAA
AAAAAGAAAGAATGCAGTGAAACTGAGCTGAGTAATCGTTTTAAAAATGTTGAGCTTCAG
GCTGCACAATTGACAAAATCAACAGAATCATTGAATGCAGTTGAAGTTCCTCAATCGATA
AGTCATTATGTAGAAGATCCAACTTATGCATATCAAGATTTTGCTAGACGTGGAGCTATA
TTCCACATACTTTTCGAATCCAAGATTATTCTTGGGATGATCATGGCTATTCACTTGTCA
ATCGTCTTTATAATGACGTTGGATTTCTGCTAGATGATAAATTCCGTGTCGCTTACAACT
TAACTTATAAGACACTCGCTGGTCGTCAAAATGTCGACACATCGAAATTTCGTCGTGCAA
TATGGAATTATATTCAGTGCATCTATGGAATCAGACATGATGATTATGACTATGGCGAAA
TCAATCAACTTCTTGATCGTACTCTCAATTCATTCATCAAAACAGCTTGTTGCTTCCCAG
AGCGAATAACAAAGCAAGATTATGACAATGTTCTCGTGGAATTACTTGACAGTGAAAAAG
TTCACGTTAATTTGATGATAATGGAGGCAAAAAATCAAGCGATATTGCTTTATGCATTGA
GAGAAATAATGCGCTATATGCTATGATAAATTGATCTCATTTCATTTTGGATTCATTGAA
GAAATTTGCTATTCATATTTGATCAAAAAATACAAAACACACAAAAAATTAATTAATTAA
TCCTAAAATATACTATTTTTACAAAATGCAGTAAGTAAGTGTTCGTTGAGCTTTTATCGT
ACAAGTCTTGTGCGATAAGTTACAAGAAATAAATAAGTAATAAATGTCGATATTGTTAAG
ATGATTTTTTTAAGAAAGACGACGAAGAAGAAGTAACAAAGAAATAATAATTATTATAAA
TATTTTTTCACCATAAATATGATAGAGTGTTACAATATATATACATTTATATATCTTATA
GCTATTTGTGATGATAGATCGTATGATGTAAAAGGAGAAGCGACATGTTTTTCTTCTTCA
GTTTTCTTTTTTTTTATATTCGTTAAGAGGAAAATGTTGTTTTGTGATTTTTTTTCTATG
AAGAAAACTAAGCGAGATAATTGATCAACACGTGCCAAAATTTTAAGAAGAAAAAATAAA
GAGAAAAAACGATGATTTCAAAGAATA
>g7477.t5 Gene=g7477 Length=128
MMKEQEEQSKKVLQQNNSNGTSSDNIEMLMKRMQDLSEKKKECSETELSNRFKNVELQAA
QLTKSTESLNAVEVPQSISHYVEDPTYAYQDFARRGAIFHILFESKIILGMIMAIHLSIV
FIMTLDFC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g7477.t5 | Coils | Coil | Coil | 26 | 46 | - |
| 4 | g7477.t5 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 41 | - |
| 3 | g7477.t5 | MobiDBLite | mobidb-lite | consensus disorder prediction | 11 | 28 | - |
| 1 | g7477.t5 | Pfam | PF04636 | PA26 p53-induced protein (sestrin) | 6 | 97 | 8.1E-14 |
| 6 | g7477.t5 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 106 | - |
| 8 | g7477.t5 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 107 | 125 | - |
| 7 | g7477.t5 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 126 | 128 | - |
| 2 | g7477.t5 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 101 | 123 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005634 | nucleus | CC |
| GO:1901031 | regulation of response to reactive oxygen species | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed