General information for g7504.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Zinc metalloproteinase nas-4.
Parent gene
- The parent gene g7504 is located on chr_2: 24101743..24102764 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7504 | g7504.t1 | isoform | g7504.t1 | 24101743 | 24102764 |
| chr_2 | g7504 | g7504.t1 | exon | g7504.t1.exon1 | 24101743 | 24102171 |
| chr_2 | g7504 | g7504.t1 | cds | g7504.t1.CDS1 | 24101743 | 24102171 |
| chr_2 | g7504 | g7504.t1 | exon | g7504.t1.exon2 | 24102237 | 24102349 |
| chr_2 | g7504 | g7504.t1 | cds | g7504.t1.CDS2 | 24102237 | 24102349 |
| chr_2 | g7504 | g7504.t1 | exon | g7504.t1.exon3 | 24102413 | 24102604 |
| chr_2 | g7504 | g7504.t1 | cds | g7504.t1.CDS3 | 24102413 | 24102604 |
| chr_2 | g7504 | g7504.t1 | exon | g7504.t1.exon4 | 24102662 | 24102764 |
| chr_2 | g7504 | g7504.t1 | cds | g7504.t1.CDS4 | 24102662 | 24102764 |
| chr_2 | g7504 | g7504.t1 | TSS | g7504.t1 | NA | NA |
| chr_2 | g7504 | g7504.t1 | TTS | g7504.t1 | NA | NA |
Sequences
>g7504.t1 Gene=g7504 Length=837
ATGGCAGAGATTTATTTCTTCTTCATTTTATTATCGATATTTTTCAATAAAACTTTTCCA
TTTCCAAGTAAAAGTTTTATACAACGAAATAGTCATGAAAATATGCTTCGCTTGATGAAT
CTCAGTCCATATGAAAATGCAGAAGAATTAAGTGGCCAATTTGAAGGTGACATAATTATT
TCACCAAAGCAGCTTAATGAACTTTTCAAACAAAATTCAAAGACTGGTTTAATTGACTTA
AAGTATCGTTGGACTAACAATATTGTGCCATATCAAATTAAAGTTAAAGATTTTGATACA
GCACAAATTCATCATATTGAAAATGCCATAAGAACCCTCGAGAATGCAACGTGCATAAAA
TTTGTTGAATATCAAGCTGATGTTCATGTTAATTATGTTTGGGTTACTGGCGAAGATGGT
GGATGTTATTCAAGTGTTGGCATGCAAGGTGGTCGTCAACGATTGAATCTACAATCATAT
AAAATTGAATCAGGTTGCTTTCGTCTTTATACTATAGTACATGAATTTATTCATGCTTTA
GGCTTTTATCACATGCAAAGTGCATCGAATCGAGATGATTATGTGAAAATTGTTTGGAAA
AACATTCAAGATGGTGCTGAGCACAACTTTAATGCTTATTCGGCTGACAAAATCCATATG
TATGGTGAACCATATGACTATGAATCAGTTATGCATTATTCAGACACTGCCTTTTCAAAA
AATGGTGAGAAAACCATTATTCCATTAACAGAACTCAATGATGGAGTCACAATGGGACAA
AGAGTAAAAATGACACAGCATGATATAGATCGAATTAATGCCATGTATTGTAAATAA
>g7504.t1 Gene=g7504 Length=278
MAEIYFFFILLSIFFNKTFPFPSKSFIQRNSHENMLRLMNLSPYENAEELSGQFEGDIII
SPKQLNELFKQNSKTGLIDLKYRWTNNIVPYQIKVKDFDTAQIHHIENAIRTLENATCIK
FVEYQADVHVNYVWVTGEDGGCYSSVGMQGGRQRLNLQSYKIESGCFRLYTIVHEFIHAL
GFYHMQSASNRDDYVKIVWKNIQDGAEHNFNAYSADKIHMYGEPYDYESVMHYSDTAFSK
NGEKTIIPLTELNDGVTMGQRVKMTQHDIDRINAMYCK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 17 | g7504.t1 | CDD | cd04280 | ZnMc_astacin_like | 86 | 276 | 1.98169E-84 |
| 11 | g7504.t1 | Gene3D | G3DSA:3.40.390.10 | Collagenase (Catalytic Domain) | 34 | 278 | 6.2E-70 |
| 2 | g7504.t1 | PANTHER | PTHR10127:SF780 | ASTACIN-LIKE METALLOENDOPEPTIDASE | 33 | 277 | 1.3E-65 |
| 3 | g7504.t1 | PANTHER | PTHR10127 | DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAINING | 33 | 277 | 1.3E-65 |
| 5 | g7504.t1 | PRINTS | PR00480 | Astacin family signature | 106 | 124 | 2.8E-25 |
| 7 | g7504.t1 | PRINTS | PR00480 | Astacin family signature | 166 | 184 | 2.8E-25 |
| 4 | g7504.t1 | PRINTS | PR00480 | Astacin family signature | 185 | 202 | 2.8E-25 |
| 6 | g7504.t1 | PRINTS | PR00480 | Astacin family signature | 224 | 239 | 2.8E-25 |
| 8 | g7504.t1 | PRINTS | PR00480 | Astacin family signature | 264 | 277 | 2.8E-25 |
| 1 | g7504.t1 | Pfam | PF01400 | Astacin (Peptidase family M12A) | 83 | 278 | 2.2E-56 |
| 13 | g7504.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 14 | g7504.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 15 | g7504.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 16 | g7504.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 20 | - |
| 12 | g7504.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 278 | - |
| 19 | g7504.t1 | ProSiteProfiles | PS51864 | Astacin-like domain profile. | 75 | 278 | 72.117 |
| 18 | g7504.t1 | SMART | SM00235 | col_5 | 80 | 229 | 9.9E-38 |
| 9 | g7504.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 80 | 277 | 7.58E-54 |
| 10 | g7504.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0004222 | metalloendopeptidase activity | MF |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed