General information for g752.t27
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase.
Parent gene
- The parent gene g752 is located on chr_3: 5737587..5738609 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g752 | g752.t27 | TTS | g752.t27 | 5736461 | 5736461 |
| chr_3 | g752 | g752.t27 | isoform | g752.t27 | 5736518 | 5738609 |
| chr_3 | g752 | g752.t27 | exon | g752.t27.exon1 | 5736518 | 5738286 |
| chr_3 | g752 | g752.t27 | cds | g752.t27.CDS1 | 5736518 | 5738152 |
| chr_3 | g752 | g752.t27 | exon | g752.t27.exon2 | 5738357 | 5738493 |
| chr_3 | g752 | g752.t27 | exon | g752.t27.exon3 | 5738571 | 5738609 |
| chr_3 | g752 | g752.t27 | TSS | g752.t27 | 5738761 | 5738761 |
Sequences
>g752.t27 Gene=g752 Length=1945
ATGCACGATATTTTATATCATAAATTTAATTTGACTCAGTATGACCATCATGAGTTTCCT
GGTGTTGTGCCTAGAACTTTTCTAGGACCACTTTTGATTTCTCTTGTCATTTCACCTGTA
GTTGCATTATTCAATATACTTAGCATTAATAAGTTTTGGTCTCAATATTTGGGTATTACG
ACTGACACTTGCTGCATTTGTATCGCTTGCATTCAATCGATTACGAAGGACATTAAACCA
AAAGTTTGGAAATGATTTTTCAATTTGGTTCTTAGCTATTCATCTTTCACAATTCCATTT
CATATTCTACATGAGTAGACCGCTGCCAAATGTTATGACTTTACCATTTGTGCTACTTGC
CGTCGATTCATGGCTTAAAAGAGAACAAAGGGGATTTTTAGTTTTTTCTGGAATAGCAAT
TCTAATTTTTAGATCGGAGCTCGTAATCTTTTTAGGTCTTTTATTAATCTACGATCTCTT
TTATAAACGAATAACAATACCAGAATTATTAAAACTTGGTGTGCCAATAGGTGCTATTCT
TGTTTTATTAACTGTCATTGTTGATTCAATATTTTGGCAAAAACCATTGTGGCCAGAAGC
TCAAGTTTTCTGGTATAATACTATTCTGAATAAATCATCTGATTGGGGCACATCTCCCTT
TTTATGGTATTTTTATTCTGCGCTACCTAGAGCTCTCGGTTTATCGCTTTTACTTGTTCC
TTATGGACTATATGCTGAAGCACGCATACGTGCAATAACTATACCAGCTTTATTATTTGT
ACTCCTTTATTCATTTCTTCCACATAAGGAGCTCAGATTTATCATCTACGTTTTTCCAAT
GCTTAATATAGCAAGTGCATGTGCATGTCAACGATTCTGGCAAAATCGAAATAAATCAAT
TTTTCGTGGAATGCTAGCAGTTTTCGCTATTGCACATCTTATTGGAAATTTCATATTGAC
AGTTTTTCTGTTAACCGTCTCGAAAAATAATTATCCTGGTGGTGTAGCAATCTCAAGACT
TCACCGAATTGCATCAAATGAAATGAATGTAACGATACATATTGATAATTTAACTGCTCA
AACTGGTGTAACAAGATTTACTGAAATCTACTCAAATTGGACTTATCGAAAGGATGAAAA
TTTGAAGGCTGGTGATGCAGAATTGCATAATTTTAATTACTTACTTGTGGAAGTAAAAAA
CAGATTTTCTCCAGAAATGAAATTACTTGCGCAAACACACGAAAAAATTGAACTGATTGA
GTGTTTTAGTAATATAGGTGTGCATTATGCATCATTTTTTCCAATAAGAATAAGGACAAA
ACCTTGTATCATGATTATGAAAAAGAAAAAAAATGGAATTTTATTGAAAAGATATTTTGA
GGTTGAAGAAGAAAGTGATGGCAATAATGAATTTCTAAATGAAGAATTTCCTGACGAAAA
TGAAAATTTAACAATAAACTTTGAGGAAGAAAAGGAGCATGAAGAAAAGGAAGAAAATGA
AGAAGAAGAAGAAGTTATTACTGAGGCACCTCCGAAAATTCTCAAAAGAAAACGTTTGCG
ATCATCGATTTTAAAGATGAAAATTAAAGAAATAATTGAAGCTGGAAAATTAGAAAACAT
GGATATTCAAAATGATGATGACACTATAGAGCAAATCTCGCAAGAAAATCAAGACATACA
GATAAATGATGATTTGCCAGAAGAAGAAAATGATTTTTCAATCGAGTCGGAAATAAATCA
AGATGATGAAGAGAGTGAAACTGTAGATAAGAATGATATAGGTAGTGGAAAAACTAAAGA
TAATATAAAGAAAATTATTTCTAAAGAAAAGACAAAACAACTGCTTGATGAACTTAAGAA
AATTGATTTAAGTGCCTTGTGCGATTTGGAGCAAATGGACACTAAAGAGTGTTTGAAAAA
AATTCTTGATGAAAATGTAGAATGA
>g752.t27 Gene=g752 Length=544
MSRPLPNVMTLPFVLLAVDSWLKREQRGFLVFSGIAILIFRSELVIFLGLLLIYDLFYKR
ITIPELLKLGVPIGAILVLLTVIVDSIFWQKPLWPEAQVFWYNTILNKSSDWGTSPFLWY
FYSALPRALGLSLLLVPYGLYAEARIRAITIPALLFVLLYSFLPHKELRFIIYVFPMLNI
ASACACQRFWQNRNKSIFRGMLAVFAIAHLIGNFILTVFLLTVSKNNYPGGVAISRLHRI
ASNEMNVTIHIDNLTAQTGVTRFTEIYSNWTYRKDENLKAGDAELHNFNYLLVEVKNRFS
PEMKLLAQTHEKIELIECFSNIGVHYASFFPIRIRTKPCIMIMKKKKNGILLKRYFEVEE
ESDGNNEFLNEEFPDENENLTINFEEEKEHEEKEENEEEEEVITEAPPKILKRKRLRSSI
LKMKIKEIIEAGKLENMDIQNDDDTIEQISQENQDIQINDDLPEEENDFSIESEINQDDE
ESETVDKNDIGSGKTKDNIKKIISKEKTKQLLDELKKIDLSALCDLEQMDTKECLKKILD
ENVE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g752.t27 | Coils | Coil | Coil | 377 | 402 | - |
| 10 | g752.t27 | MobiDBLite | mobidb-lite | consensus disorder prediction | 474 | 493 | - |
| 2 | g752.t27 | PANTHER | PTHR22760:SF1 | DOL-P-MAN:MAN(7)GLCNAC(2)-PP-DOL ALPHA-1,6-MANNOSYLTRANSFERASE | 1 | 346 | 3.0E-120 |
| 3 | g752.t27 | PANTHER | PTHR22760 | GLYCOSYLTRANSFERASE | 1 | 346 | 3.0E-120 |
| 1 | g752.t27 | Pfam | PF03901 | Alg9-like mannosyltransferase family | 1 | 252 | 5.4E-32 |
| 19 | g752.t27 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 20 | g752.t27 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 7 | - |
| 21 | g752.t27 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 8 | 16 | - |
| 28 | g752.t27 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 20 | - |
| 16 | g752.t27 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 29 | - |
| 23 | g752.t27 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 30 | 54 | - |
| 12 | g752.t27 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 55 | 65 | - |
| 27 | g752.t27 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 66 | 89 | - |
| 15 | g752.t27 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 90 | 116 | - |
| 22 | g752.t27 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 117 | 136 | - |
| 14 | g752.t27 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 137 | 147 | - |
| 25 | g752.t27 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 148 | 164 | - |
| 17 | g752.t27 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 165 | 169 | - |
| 26 | g752.t27 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 170 | 190 | - |
| 13 | g752.t27 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 191 | 201 | - |
| 24 | g752.t27 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 202 | 223 | - |
| 18 | g752.t27 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 224 | 544 | - |
| 5 | g752.t27 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 31 | 53 | - |
| 4 | g752.t27 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 66 | 88 | - |
| 6 | g752.t27 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 117 | 139 | - |
| 8 | g752.t27 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 146 | 163 | - |
| 7 | g752.t27 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 168 | 190 | - |
| 9 | g752.t27 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 202 | 224 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005788 | endoplasmic reticulum lumen | CC |
| GO:0016757 | glycosyltransferase activity | MF |
| GO:0052824 | NA | NA |
| GO:0006488 | dolichol-linked oligosaccharide biosynthetic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
