General information for g7543.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Follistatin.
Parent gene
- The parent gene g7543 is located on chr_2: 24439049..24447710 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7543 | g7543.t1 | TTS | g7543.t1 | 24424869 | 24424869 |
| chr_2 | g7543 | g7543.t1 | isoform | g7543.t1 | 24425435 | 24439049 |
| chr_2 | g7543 | g7543.t1 | exon | g7543.t1.exon1 | 24425435 | 24425557 |
| chr_2 | g7543 | g7543.t1 | cds | g7543.t1.CDS1 | 24425435 | 24425557 |
| chr_2 | g7543 | g7543.t1 | exon | g7543.t1.exon2 | 24428393 | 24428505 |
| chr_2 | g7543 | g7543.t1 | cds | g7543.t1.CDS2 | 24428393 | 24428505 |
| chr_2 | g7543 | g7543.t1 | exon | g7543.t1.exon3 | 24433280 | 24433519 |
| chr_2 | g7543 | g7543.t1 | cds | g7543.t1.CDS3 | 24433280 | 24433519 |
| chr_2 | g7543 | g7543.t1 | exon | g7543.t1.exon4 | 24434643 | 24434775 |
| chr_2 | g7543 | g7543.t1 | cds | g7543.t1.CDS4 | 24434643 | 24434775 |
| chr_2 | g7543 | g7543.t1 | exon | g7543.t1.exon5 | 24434844 | 24435274 |
| chr_2 | g7543 | g7543.t1 | cds | g7543.t1.CDS5 | 24434844 | 24435274 |
| chr_2 | g7543 | g7543.t1 | exon | g7543.t1.exon6 | 24435334 | 24435513 |
| chr_2 | g7543 | g7543.t1 | cds | g7543.t1.CDS6 | 24435334 | 24435513 |
| chr_2 | g7543 | g7543.t1 | exon | g7543.t1.exon7 | 24439001 | 24439049 |
| chr_2 | g7543 | g7543.t1 | cds | g7543.t1.CDS7 | 24439001 | 24439049 |
| chr_2 | g7543 | g7543.t1 | TSS | g7543.t1 | 24439284 | 24439284 |
Sequences
>g7543.t1 Gene=g7543 Length=1269
ATGATTTTTGTGATAATTTTGACGAGCAATTTTTTATTGTGTTGTAATGCTGGATCGTGC
TTTTCAAATATCAATGCAGAAGGAAAATGTAGTGGTCTAACTGCCAATGATATCTCAAAA
GAAGAATGTTGCTCAGTTCTAGGAGTTGCATATGGAGAACCAATTTCAAATGAAGCAATT
TTTTGGGTGCATGCTGGAATTAATAGAGAAACATGTAAACCATGCAAAGAATCTTGTGAC
AACGTAAAGTGCGGTGTTGGTAAGCGATGCATCATGAAAAGAGGCCAACCAAAATGCATT
TGTTCACCACAATGTAAAGCGGCGACCGCAGCGATTAATAAAAATCGTAAAGTAGGCGTC
AACGAAGAATTTACAACAGCTCTTGAAATGCCCGAAATGAGAAGCGTAAATCGTCATTAT
ACTGTCATTCGACCTCCAATTATATCGGAAATGCAAAATGATGAGCCTACACTCGAAAAG
AATTTTGCTAATAAGAATTTGAAAAAAATTAACCAAAGTATCGAGGCTTTTCATCTTTTC
TTCAATAATAGTAATGCCGCCAATAAAACACTTGAGTTGAAATTTCGAAATAAACTTTTA
ACTCACAACAATAGTAACAATAATATGCTTCATTTCGATGAATTTTATCTTGGTAACATT
CCAAAATTTGCACAATATAGTCCCGTTTGCGGTACCGATGGAAAAACTTATAAAAATGAA
TGTCAATTGCAGAAACGTTCATGCAGGCAAGAGAATAAAAAGCTGGAGGTTTCACATAAA
GGTTTTTGTCAAAGTTCATGCAAATTCATCAACTGTCTTAATGGAAAGCAATGCGTTGAG
GATCAGAACTTTATGCCGCAATGTATTAGCTGCTCAAAATGTTCTCGAAAGAATCGGACC
CTTTCGCAGTTGGATCTCAAGAAATTTGTGTGTGGTGTTGATGGTATTACATACAGAAGC
CTCTGTGAGTTAAAACAAAAATCGTGCCGAGTCGGAAAGTCCATTCAACTGTCACATCGT
GGACCATGCACAGAATCGCTGAGTTGCAATCAATGCAAGAGAACAGAACGATGTCTACCG
GATCTTATCACAAGCAAGCCAGTTTGTGTATCTTGTAAACGACCTAATGGCCGTTGCAGC
ATTGAGAATGTGAAGAAAGTTTGCGGCAACAATAACATCACATATCGTTCGAAATGTCAC
TTAATGAGAGACTCTTGTAATACTGGATTTGCTATTAGCGTAAAACACGAAGGAACATGC
AGATCATGA
>g7543.t1 Gene=g7543 Length=422
MIFVIILTSNFLLCCNAGSCFSNINAEGKCSGLTANDISKEECCSVLGVAYGEPISNEAI
FWVHAGINRETCKPCKESCDNVKCGVGKRCIMKRGQPKCICSPQCKAATAAINKNRKVGV
NEEFTTALEMPEMRSVNRHYTVIRPPIISEMQNDEPTLEKNFANKNLKKINQSIEAFHLF
FNNSNAANKTLELKFRNKLLTHNNSNNNMLHFDEFYLGNIPKFAQYSPVCGTDGKTYKNE
CQLQKRSCRQENKKLEVSHKGFCQSSCKFINCLNGKQCVEDQNFMPQCISCSKCSRKNRT
LSQLDLKKFVCGVDGITYRSLCELKQKSCRVGKSIQLSHRGPCTESLSCNQCKRTERCLP
DLITSKPVCVSCKRPNGRCSIENVKKVCGNNNITYRSKCHLMRDSCNTGFAISVKHEGTC
RS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 24 | g7543.t1 | CDD | cd00104 | KAZAL_FS | 225 | 263 | 1.36527E-7 |
| 26 | g7543.t1 | CDD | cd00104 | KAZAL_FS | 310 | 343 | 1.9055E-7 |
| 25 | g7543.t1 | CDD | cd00104 | KAZAL_FS | 382 | 420 | 1.74986E-6 |
| 15 | g7543.t1 | Gene3D | G3DSA:3.90.290.10 | Extracellular Matrix Fibrillin | 18 | 108 | 3.2E-20 |
| 18 | g7543.t1 | Gene3D | G3DSA:3.30.60.30 | - | 221 | 265 | 4.1E-15 |
| 16 | g7543.t1 | Gene3D | G3DSA:3.30.60.30 | - | 266 | 343 | 3.9E-17 |
| 17 | g7543.t1 | Gene3D | G3DSA:3.30.60.30 | - | 348 | 420 | 7.9E-14 |
| 6 | g7543.t1 | PANTHER | PTHR13866 | SPARC OSTEONECTIN | 15 | 110 | 3.3E-66 |
| 9 | g7543.t1 | PANTHER | PTHR13866:SF22 | FOLLISTATIN | 15 | 110 | 3.3E-66 |
| 5 | g7543.t1 | PANTHER | PTHR13866 | SPARC OSTEONECTIN | 227 | 374 | 3.3E-66 |
| 8 | g7543.t1 | PANTHER | PTHR13866:SF22 | FOLLISTATIN | 227 | 374 | 3.3E-66 |
| 4 | g7543.t1 | PANTHER | PTHR13866 | SPARC OSTEONECTIN | 302 | 421 | 3.3E-66 |
| 7 | g7543.t1 | PANTHER | PTHR13866:SF22 | FOLLISTATIN | 302 | 421 | 3.3E-66 |
| 1 | g7543.t1 | Pfam | PF07648 | Kazal-type serine protease inhibitor domain | 224 | 263 | 3.0E-10 |
| 3 | g7543.t1 | Pfam | PF07648 | Kazal-type serine protease inhibitor domain | 307 | 343 | 4.2E-8 |
| 2 | g7543.t1 | Pfam | PF07648 | Kazal-type serine protease inhibitor domain | 378 | 420 | 4.5E-10 |
| 20 | g7543.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 21 | g7543.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 1 | - |
| 22 | g7543.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 2 | 13 | - |
| 23 | g7543.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 17 | - |
| 19 | g7543.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 422 | - |
| 36 | g7543.t1 | ProSiteProfiles | PS51364 | TGF-beta binding (TB) domain profile. | 18 | 75 | 9.853 |
| 34 | g7543.t1 | ProSiteProfiles | PS51465 | Kazal domain profile. | 226 | 265 | 10.177 |
| 35 | g7543.t1 | ProSiteProfiles | PS51465 | Kazal domain profile. | 289 | 345 | 9.569 |
| 33 | g7543.t1 | ProSiteProfiles | PS51465 | Kazal domain profile. | 370 | 422 | 10.953 |
| 32 | g7543.t1 | SMART | SM00274 | FOLN_3 | 78 | 100 | 1.7E-4 |
| 28 | g7543.t1 | SMART | SM00280 | kazal_3 | 221 | 263 | 1.2E-9 |
| 31 | g7543.t1 | SMART | SM00274 | FOLN_3 | 266 | 289 | 1.4 |
| 29 | g7543.t1 | SMART | SM00280 | kazal_3 | 290 | 343 | 2.2E-8 |
| 30 | g7543.t1 | SMART | SM00274 | FOLN_3 | 348 | 370 | 98.0 |
| 27 | g7543.t1 | SMART | SM00280 | kazal_3 | 368 | 420 | 3.8E-7 |
| 10 | g7543.t1 | SUPERFAMILY | SSF100895 | Kazal-type serine protease inhibitors | 224 | 263 | 4.57E-12 |
| 12 | g7543.t1 | SUPERFAMILY | SSF100895 | Kazal-type serine protease inhibitors | 306 | 343 | 3.6E-10 |
| 11 | g7543.t1 | SUPERFAMILY | SSF100895 | Kazal-type serine protease inhibitors | 378 | 420 | 1.52E-9 |
| 14 | g7543.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
| 13 | g7543.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 26 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
- This transcript has been annotated with K04661 (follistatin)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
