Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g7588 g7588.t6 TTS g7588.t6 24742066 24742066
chr_2 g7588 g7588.t6 isoform g7588.t6 24742231 24743704
chr_2 g7588 g7588.t6 exon g7588.t6.exon1 24742231 24742364
chr_2 g7588 g7588.t6 cds g7588.t6.CDS1 24742305 24742364
chr_2 g7588 g7588.t6 exon g7588.t6.exon2 24742444 24743127
chr_2 g7588 g7588.t6 cds g7588.t6.CDS2 24742444 24743127
chr_2 g7588 g7588.t6 exon g7588.t6.exon3 24743579 24743704
chr_2 g7588 g7588.t6 cds g7588.t6.CDS3 24743579 24743704
chr_2 g7588 g7588.t6 TSS g7588.t6 24743809 24743809

Sequences

>g7588.t6 Gene=g7588 Length=944
ATGGCATTCCGTATAATAAAATTGACTCCAAATTTTGCACGCATGATGTCAACAAGTGCA
ACAAAAGTTGGGTTCATTGGTTTGGGACAAATGGGAAATCGTATGGCTACAAATCTAATC
AATAAGGGCTACAAATTGAATGTCTTTGATGTTTCACCAGAAGCATGTGATGCGATGAAA
GCAAAAGGTGCTCAAGTCTACAAAACCACACAAGAGTTGGCAAAGGAGTCAGAATTTGTT
GTCACAATGCTTCCTAATAATGATATCGTAAAAGAAACATATGAAGTCATGATTAAGGAT
GGTGTAAATAACAAAACAATGTTTGTCGATTCCTCAACAGTTGATCCATCAGTCTCACAG
TATGTGCAAAAAATTGTATCTAAAGCTGGCGCCAGATTTGTTGACGCTCCAGTATCGGGA
GGTGTCATGGGTGCTCAGAATGCAACCTTGACTTTCATGGTTGGTGGTACAAAAGAGGAA
TACGAACATGTCAAGGACTTTTTAGCCGGAATGGGAAAGAAAATTACACATTGTGGCACA
TATGGTATGGGTGAAGCTGCTAAAATTTGCAATAACATGATGTTGGCTATCAGTATGATT
GGAACAGCTGAAACGATGAATTTGGCATTACGCCTTGGTTTAGATGCAAAAGTATTTAAT
GAAATTATCAACTCATCAACGGGACGTTCATGGGCATCAGAAATTAATAATCCTGTGCCA
GGACTCAATCCAGGCACTCCACCAAGTGATGGCTATAAAGGCGGTTTCTCGACAGCACTC
ATTACCAAGGACTTGGGCTTGGCAAGCAGTCAACTGGCTCGAATTCTCCCATCATGCTTG
GCGCCCTTGCTCATCACATTTATCGTTTAATGTGTACCCACGATCTGGGCAATAAGGACT
TTTCAGTTATATACGACTTCATTAAAAACAAGTCAGGAAAATAA

>g7588.t6 Gene=g7588 Length=289
MAFRIIKLTPNFARMMSTSATKVGFIGLGQMGNRMATNLINKGYKLNVFDVSPEACDAMK
AKGAQVYKTTQELAKESEFVVTMLPNNDIVKETYEVMIKDGVNNKTMFVDSSTVDPSVSQ
YVQKIVSKAGARFVDAPVSGGVMGAQNATLTFMVGGTKEEYEHVKDFLAGMGKKITHCGT
YGMGEAAKICNNMMLAISMIGTAETMNLALRLGLDAKVFNEIINSSTGRSWASEINNPVP
GLNPGTPPSDGYKGGFSTALITKDLGLASSQLARILPSCLAPLLITFIV

Protein features from InterProScan

Transcript Database ID Name Start End E.value
8 g7588.t6 Gene3D G3DSA:3.40.50.720 - 17 180 4.2E-54
9 g7588.t6 Gene3D G3DSA:1.10.1040.10 - 182 278 1.6E-29
3 g7588.t6 PANTHER PTHR22981 3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED 11 271 7.6E-102
4 g7588.t6 PANTHER PTHR22981:SF7 3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL 11 271 7.6E-102
10 g7588.t6 PIRSF PIRSF000103 HIBADH 15 282 3.2E-70
2 g7588.t6 Pfam PF03446 NAD binding domain of 6-phosphogluconate dehydrogenase 22 179 1.8E-49
1 g7588.t6 Pfam PF14833 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase 182 272 3.4E-21
11 g7588.t6 ProSitePatterns PS00895 3-hydroxyisobutyrate dehydrogenase signature. 25 38 -
5 g7588.t6 SUPERFAMILY SSF51735 NAD(P)-binding Rossmann-fold domains 21 180 2.25E-44
6 g7588.t6 SUPERFAMILY SSF48179 6-phosphogluconate dehydrogenase C-terminal domain-like 181 270 5.78E-25
7 g7588.t6 SignalP_GRAM_POSITIVE SignalP-TM SignalP-TM 1 15 -
12 g7588.t6 TIGRFAM TIGR01692 HIBADH: 3-hydroxyisobutyrate dehydrogenase 25 271 1.2E-94

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0050661 NADP binding MF
GO:0008442 3-hydroxyisobutyrate dehydrogenase activity MF
GO:0051287 NAD binding MF
GO:0055114 NA NA
GO:0016491 oxidoreductase activity MF

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values