General information for g7647.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Carbonic anhydrase 15.
Parent gene
- The parent gene g7647 is located on chr_2: 25055183..25057607 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7647 | g7647.t1 | isoform | g7647.t1 | 25055183 | 25057607 |
| chr_2 | g7647 | g7647.t1 | exon | g7647.t1.exon1 | 25055183 | 25055364 |
| chr_2 | g7647 | g7647.t1 | cds | g7647.t1.CDS1 | 25055183 | 25055364 |
| chr_2 | g7647 | g7647.t1 | exon | g7647.t1.exon2 | 25055436 | 25055511 |
| chr_2 | g7647 | g7647.t1 | cds | g7647.t1.CDS2 | 25055436 | 25055511 |
| chr_2 | g7647 | g7647.t1 | exon | g7647.t1.exon3 | 25055792 | 25055842 |
| chr_2 | g7647 | g7647.t1 | cds | g7647.t1.CDS3 | 25055792 | 25055842 |
| chr_2 | g7647 | g7647.t1 | exon | g7647.t1.exon4 | 25055905 | 25056233 |
| chr_2 | g7647 | g7647.t1 | cds | g7647.t1.CDS4 | 25055905 | 25056233 |
| chr_2 | g7647 | g7647.t1 | exon | g7647.t1.exon5 | 25056299 | 25056310 |
| chr_2 | g7647 | g7647.t1 | cds | g7647.t1.CDS5 | 25056299 | 25056310 |
| chr_2 | g7647 | g7647.t1 | exon | g7647.t1.exon6 | 25056379 | 25056468 |
| chr_2 | g7647 | g7647.t1 | cds | g7647.t1.CDS6 | 25056379 | 25056468 |
| chr_2 | g7647 | g7647.t1 | exon | g7647.t1.exon7 | 25056935 | 25057090 |
| chr_2 | g7647 | g7647.t1 | cds | g7647.t1.CDS7 | 25056935 | 25057090 |
| chr_2 | g7647 | g7647.t1 | exon | g7647.t1.exon8 | 25057160 | 25057195 |
| chr_2 | g7647 | g7647.t1 | cds | g7647.t1.CDS8 | 25057160 | 25057195 |
| chr_2 | g7647 | g7647.t1 | exon | g7647.t1.exon9 | 25057559 | 25057607 |
| chr_2 | g7647 | g7647.t1 | cds | g7647.t1.CDS9 | 25057559 | 25057607 |
| chr_2 | g7647 | g7647.t1 | TSS | g7647.t1 | NA | NA |
| chr_2 | g7647 | g7647.t1 | TTS | g7647.t1 | NA | NA |
Sequences
>g7647.t1 Gene=g7647 Length=981
ATGATTGCAATTTATATACTTGCGATAAATCTCATTACAATATCAGTTGTAAATGCAAAT
GAATTCTCGTATGATGGGAAAACAGGACCTGAAAACTGGGACAAAACATGCAAGGGAAAA
CATCAAAGTCCTATTGATATCGATGTCCTGCATGTGAAAAAAGTGAAATTACCACCATTA
AAATTTGAAAACTTTGACATTCAACCTGAAAGTACCACTGTCACAAACAATGGACATACA
GTTATGTTCACAGTGAAAACAGCAAAAGGCAGCGAGCCAACTATAGATGGTGGACCATTC
AAGACTGGCAAATATGTTTTCAGTCAGCTACATTTTCATTGGGGCGATAATGACACATAT
GGTTCGGAAGATAGCTTCAATGGAAAACACTTTCCTATGGAATTACATGTTGTGTTCTAC
AAACAAAAATATGGTACACAAGAAAATGCATTAAAACATGAGGATGGACTTGCAGTGCTT
GCTTTTTTCTTTGTGATTGCACAAAAACCTAATCCATCCTATGTTGAAGTTTCTAAACTT
TTAAAAAGAATTATAAGTCCCAACACTAATGCAACTTTTGAAGATCCATTAGCTCTAGAG
GATTACATGCATTCTAACATGCACGAATACTATGTTTACAACGGTTCTCTGACAACTCCA
CCTTGTTTGGAAGTTGTCACATGGCTTGATTTTTATAATCCTATACAAATTTCTCACAGT
CAGCTCGAGAACTTCAGATCATTACAAGATAATGAAGGAAATTTATTATCGCACAACTTT
CGACCAGTGCAGCCTATTGGAGACAGAATTGTATGGTTGACCTCAAAACAGTTTGATCAT
CAAGATGAAGATTTACACGATGAAATTCACGGTCAAAAGAAGAACAAAAAGAAAAGAAGA
AAGAATAGTTCGAGCAAGGTCAATTGTTCACTCACAGCACTTTTTCTTTCAATGATGTTA
GTTTTAGAAAAAATATTTTGA
>g7647.t1 Gene=g7647 Length=326
MIAIYILAINLITISVVNANEFSYDGKTGPENWDKTCKGKHQSPIDIDVLHVKKVKLPPL
KFENFDIQPESTTVTNNGHTVMFTVKTAKGSEPTIDGGPFKTGKYVFSQLHFHWGDNDTY
GSEDSFNGKHFPMELHVVFYKQKYGTQENALKHEDGLAVLAFFFVIAQKPNPSYVEVSKL
LKRIISPNTNATFEDPLALEDYMHSNMHEYYVYNGSLTTPPCLEVVTWLDFYNPIQISHS
QLENFRSLQDNEGNLLSHNFRPVQPIGDRIVWLTSKQFDHQDEDLHDEIHGQKKNKKKRR
KNSSSKVNCSLTALFLSMMLVLEKIF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g7647.t1 | CDD | cd00326 | alpha_CA | 39 | 271 | 2.08574E-87 |
| 6 | g7647.t1 | Gene3D | G3DSA:3.10.200.10 | Carbonic Anhydrase II | 15 | 272 | 3.4E-83 |
| 12 | g7647.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 283 | 304 | - |
| 2 | g7647.t1 | PANTHER | PTHR18952 | CARBONIC ANHYDRASE | 8 | 283 | 5.4E-93 |
| 3 | g7647.t1 | PANTHER | PTHR18952:SF216 | CARBONIC ANHYDRASE 2, ISOFORM A | 8 | 283 | 5.4E-93 |
| 1 | g7647.t1 | Pfam | PF00194 | Eukaryotic-type carbonic anhydrase | 22 | 272 | 9.7E-84 |
| 7 | g7647.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 302 | - |
| 9 | g7647.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 303 | 322 | - |
| 8 | g7647.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 323 | 326 | - |
| 13 | g7647.t1 | ProSiteProfiles | PS51144 | Alpha-carbonic anhydrases profile. | 20 | 275 | 67.44 |
| 11 | g7647.t1 | SMART | SM01057 | Carb_anhydrase_2a | 22 | 275 | 6.9E-104 |
| 4 | g7647.t1 | SUPERFAMILY | SSF51069 | Carbonic anhydrase | 16 | 273 | 1.96E-76 |
| 5 | g7647.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008270 | zinc ion binding | MF |
| GO:0004089 | carbonate dehydratase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01672 (carbonic anhydrase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
