General information for g7666.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable chitinase 2.
Parent gene
- The parent gene g7666 is located on chr_2: 25179816..25180578 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7666 | g7666.t1 | TTS | g7666.t1 | 25178165 | 25178165 |
| chr_2 | g7666 | g7666.t1 | isoform | g7666.t1 | 25178408 | 25180578 |
| chr_2 | g7666 | g7666.t1 | exon | g7666.t1.exon1 | 25178408 | 25180229 |
| chr_2 | g7666 | g7666.t1 | cds | g7666.t1.CDS1 | 25178408 | 25180229 |
| chr_2 | g7666 | g7666.t1 | exon | g7666.t1.exon2 | 25180292 | 25180317 |
| chr_2 | g7666 | g7666.t1 | cds | g7666.t1.CDS2 | 25180292 | 25180317 |
| chr_2 | g7666 | g7666.t1 | exon | g7666.t1.exon3 | 25180372 | 25180578 |
| chr_2 | g7666 | g7666.t1 | cds | g7666.t1.CDS3 | 25180372 | 25180578 |
| chr_2 | g7666 | g7666.t1 | TSS | g7666.t1 | 25180631 | 25180631 |
Sequences
>g7666.t1 Gene=g7666 Length=2055
ATGATTTTAGGAGTTTTTTTATTAGTTTCTTTTGCAATTTCAAATGCACAAGACTATTGT
GGATTATCAGGGAAATACGTTGGATTAGTTGTAAATGGAACACAAACTGAAAGAGGAACA
TGGCGTTGGTTAGTTGCAATTTATAAAATTGAAGGAAATCAATTGATTTGTGGTGGAACT
TTAGTATCAGCTGATACTGTAGTAACTGCAGCTCATTGTATGCAAGACAAAAGTGTTAAA
ACACCAAGATCACCTAATGAAATTGTCATTAAACTAGGACGTTATGATTTATCAGAAAAT
TATGAACGTGGAGCTATTGATGCTTATGCAAACGATATTTTTATACATCCGAATTGGAAA
TATTATAATACAGACTATGATTCAGATATAGCTGTTATAAAATTAACCACGCCTGTTATC
TTCAATGATGTAATTATGCCAATTTGTATGTGGAGCTCATCTAGATCGAGTTCTTATCAA
ACAGGTGCTGGTATTGTTGCTGGTTTTGGAAGATCAGAAAGGGGTGGAAAACATGAGAAT
ATCGCACGTGAAATTGAAGTCAAAATCAAAACTAACGAAGAATGTTTTTTGAAGAAAGCA
GAACTTGCAAAAATTTCATCACTCAACACTTTTTGTGCAGGCAATGATCACGGTGGTGTT
TGTAGAGGAGACTCAGGATCAGGACTTTATATCAATCATCAAAATCGTTGGTATCTTCAA
GGAATTGTTTCATCATCATTAGTAGATAATAATGAATGTGATACTTCAAATGATGCAGTT
TATACCAATGTTGCAAAATTTATTGACTGGATAAATCAAATGATTGATTTTCGATTATCA
AATCTTCCTATTCCATCTGCTAACAATTTTGATGATTTCAGAAGAAACAGAAAAGAAATA
ATTTGTTATATTCCAAACTGGACCATGTACAGAAGTGAAAAATCCTTTTCATTAAATAAT
TTTAAGCCTGAACTTTGCACAACGGCTGTTTATATGTTTGCTGGTCTTGATGAAGATGGC
GGAATTAAAAGTATAGATCCTTGGGCTGATTTAGAAGATCAAGGTGGACTAAATGGGTTC
AAAAGATTTACATCATTGAAGAAAACTTTTCCACATCTTAGAACACTTTTATCAATTGGT
GGTTGGAATGAAGGAGTTGAAAAATATTCAAAATTTGTTTCGAATCCACTTAAAAGAAAA
GGTTTTGCTTTACAATCAGCTGAATTTCTTAGACAATATAATTTTGATGGATTAGATTTT
TATTGGGATTTTCCTGCTAATCCAGAACGTGGTGGAAGTCCAAATGATAAAGAAAATTTT
GTTTTACTTTTAAAAGATCTTAATGTAGTCTACTCAGCAGCTGGTCTTTTTCTTAGTGTC
ACAATGCGAGCAGCAAAATGGATAACTGATATAGCATATAATTTAACAGCTATTGCAGAT
TATGCTGATATGATGAATATGATGAATTTTGACCATAGTGGAGTTTGGGATAAGAAGATT
GGCTATAGTGCAGCACTTTATAAAAAAGCAAAATATCCATCAATTGAAGATTCAATTCAA
ATTTTTCTAAATGCAAAAGTTCCACCAAGTAAACTGACTGTTGGAATTCCTTTCTATGCA
AGAACATTTCTAACAACCGGTGATGGAAATATTGGTGATGAAAGTGAACGTGAAGCTGAA
CCTGGACCTATTATAAAAGATAATAGTTTGATTGGATTTAATGAAATTTGTTTAATGAAA
AAAGAAAAGCAATGGTTTTTTCAATTTGACCAGAAATCATCACAAATGATTGGGAAATAT
TTGACAAATGGAAAGCATGCAGTTGCTGTCTTTGATTCACCACGGTCTGTAGCGAATAAA
GTGAAATATGCAATGGAAAAGAATTTACGAGGCGTATGGGCTTGGTCAATTGATATGGAC
GACTTTGATGGAGATTGTGAAATTGATGAAACAGTTTTTAATGACTACGGTCCAAGAAAA
CCACAAATTTATCATCAGAGAGATTTTCCACTTTTACGGACAATGAATATAGCAATGAAA
TTTTTAAATAATTGA
>g7666.t1 Gene=g7666 Length=684
MILGVFLLVSFAISNAQDYCGLSGKYVGLVVNGTQTERGTWRWLVAIYKIEGNQLICGGT
LVSADTVVTAAHCMQDKSVKTPRSPNEIVIKLGRYDLSENYERGAIDAYANDIFIHPNWK
YYNTDYDSDIAVIKLTTPVIFNDVIMPICMWSSSRSSSYQTGAGIVAGFGRSERGGKHEN
IAREIEVKIKTNEECFLKKAELAKISSLNTFCAGNDHGGVCRGDSGSGLYINHQNRWYLQ
GIVSSSLVDNNECDTSNDAVYTNVAKFIDWINQMIDFRLSNLPIPSANNFDDFRRNRKEI
ICYIPNWTMYRSEKSFSLNNFKPELCTTAVYMFAGLDEDGGIKSIDPWADLEDQGGLNGF
KRFTSLKKTFPHLRTLLSIGGWNEGVEKYSKFVSNPLKRKGFALQSAEFLRQYNFDGLDF
YWDFPANPERGGSPNDKENFVLLLKDLNVVYSAAGLFLSVTMRAAKWITDIAYNLTAIAD
YADMMNMMNFDHSGVWDKKIGYSAALYKKAKYPSIEDSIQIFLNAKVPPSKLTVGIPFYA
RTFLTTGDGNIGDESEREAEPGPIIKDNSLIGFNEICLMKKEKQWFFQFDQKSSQMIGKY
LTNGKHAVAVFDSPRSVANKVKYAMEKNLRGVWAWSIDMDDFDGDCEIDETVFNDYGPRK
PQIYHQRDFPLLRTMNIAMKFLNN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 19 | g7666.t1 | CDD | cd00190 | Tryp_SPc | 30 | 273 | 2.78511E-58 |
| 12 | g7666.t1 | Gene3D | G3DSA:2.40.10.10 | - | 25 | 276 | 1.9E-58 |
| 11 | g7666.t1 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 300 | 642 | 1.1E-93 |
| 13 | g7666.t1 | Gene3D | G3DSA:3.10.50.10 | - | 538 | 611 | 1.1E-93 |
| 3 | g7666.t1 | PANTHER | PTHR11177:SF344 | CHITINASE 2-RELATED | 296 | 675 | 1.1E-79 |
| 4 | g7666.t1 | PANTHER | PTHR11177 | CHITINASE | 296 | 675 | 1.1E-79 |
| 6 | g7666.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 58 | 73 | 6.0E-10 |
| 7 | g7666.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 125 | 139 | 6.0E-10 |
| 5 | g7666.t1 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 218 | 230 | 6.0E-10 |
| 1 | g7666.t1 | Pfam | PF00089 | Trypsin | 30 | 271 | 1.5E-43 |
| 2 | g7666.t1 | Pfam | PF00704 | Glycosyl hydrolases family 18 | 300 | 640 | 2.5E-66 |
| 15 | g7666.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 16 | g7666.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 1 | - |
| 17 | g7666.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 2 | 12 | - |
| 18 | g7666.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 16 | - |
| 14 | g7666.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 684 | - |
| 22 | g7666.t1 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 68 | 73 | - |
| 23 | g7666.t1 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 30 | 276 | 26.953 |
| 20 | g7666.t1 | SMART | SM00020 | trypsin_2 | 29 | 271 | 5.0E-49 |
| 21 | g7666.t1 | SMART | SM00636 | 2g34 | 298 | 640 | 4.6E-88 |
| 8 | g7666.t1 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 10 | 276 | 1.18E-63 |
| 9 | g7666.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 298 | 648 | 2.42E-76 |
| 10 | g7666.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0008061 | chitin binding | MF |
| GO:0006508 | proteolysis | BP |
| GO:0005975 | carbohydrate metabolic process | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed