General information for g7713.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Prostaglandin E synthase 2.
Parent gene
- The parent gene g7713 is located on chr_2: 25417746..25420481 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7713 | g7713.t1 | TTS | g7713.t1 | 25416427 | 25416427 |
| chr_2 | g7713 | g7713.t1 | isoform | g7713.t1 | 25416502 | 25417746 |
| chr_2 | g7713 | g7713.t1 | exon | g7713.t1.exon1 | 25416502 | 25417533 |
| chr_2 | g7713 | g7713.t1 | cds | g7713.t1.CDS1 | 25416502 | 25417533 |
| chr_2 | g7713 | g7713.t1 | exon | g7713.t1.exon2 | 25417597 | 25417746 |
| chr_2 | g7713 | g7713.t1 | cds | g7713.t1.CDS2 | 25417597 | 25417746 |
| chr_2 | g7713 | g7713.t1 | TSS | g7713.t1 | 25417814 | 25417814 |
Sequences
>g7713.t1 Gene=g7713 Length=1182
ATGGCATTTAGATATTTATTTACAAGTGTTACAGGACGGCATGTAACTCGTTTAATTATA
CAACCAAAGAATGTAAATCTAGTGCGAAATTATGCAACAAAGATTGAGCCGAAGAAAAAA
ACTTCATCTTTAAAGCTTCTTTTAATTGGAGGAGCTATTGGAGGAACAATTGGAGCTCTC
TATTCCTTCTACGATAATATTGGCAAGAAGAAACCCACATCTAAGGATTTATCAGCTACA
ATTTTGGATAAAAAGCCGGATGTTTCAATAATGAGAAAAATTATAAATCCTAACGATAAA
AGTGATCTTGATATTGTGTTATTTCAGTTTCAAACATGTCCATTCTGTTGCAAAGTGAGA
TCATTTTTAGATTATTCTGGACTTTCTTATTCAGTTGTTGAAGTTGATGCTGTTCTTCGT
GATAGCATAAAATGGTCTGAATCAAAGAAAGTACCTATTGTACTTGTCAAATCAAAAAAT
GGCAAATATGTGCAGTTAAATGATAGTTCAATGATTATATCAGCAATTTCAAGCATATTA
GTTGATCCAGCTAAAAATGATATAGCCGAGGTTGTTAAATTTTATCCAGTTACTAATTTC
ACAGACGTCTATGGAACAATTTATGATATTGCAAATAAATATTTTCTAATGTTTCAAGAT
AAGAAACCAAAAGAAAGTAAAGAAACTTTCGATGAAGAGAGGCAATGGAGAAGCTGGGCT
GATAATCATTTGGTTCATTTAATTTCACCAAATGTCTATCGTACATATGCAGAGGCTCGA
GAAACATTTGAATGGTTTAGTGAAGTTGGACAATGGAGTGCATATTTTCCTGAGTGGGAA
CGAAATTTGATGGTACAAGTTGGTACTTTTGCTATGTGGCTTATTAGCAAACGTCTAATA
AAGAAACATGGCCTCAGTGATGATGTTCGAACTCATTTATATGATGCATGTGATAAATGG
ATGGGTGCACTCGAGAAAAAGCAAACTAAATTTATGGGTGGCAATAAAGAGCCCAATCTC
GCAGATTTATCAGTTTATGGTGTTCTTTGTAGTATGGAAGGATGTGAAGCTTTTAAAGAT
TGTCTAGAAAACACTAAAATTGGATTGTGGTTTTATGAAGTTAAAGCTAAAGTAGAAAGT
AATCGTGGTTTTAGAAAACCCACAAGTCTTTATGCTGTATGA
>g7713.t1 Gene=g7713 Length=393
MAFRYLFTSVTGRHVTRLIIQPKNVNLVRNYATKIEPKKKTSSLKLLLIGGAIGGTIGAL
YSFYDNIGKKKPTSKDLSATILDKKPDVSIMRKIINPNDKSDLDIVLFQFQTCPFCCKVR
SFLDYSGLSYSVVEVDAVLRDSIKWSESKKVPIVLVKSKNGKYVQLNDSSMIISAISSIL
VDPAKNDIAEVVKFYPVTNFTDVYGTIYDIANKYFLMFQDKKPKESKETFDEERQWRSWA
DNHLVHLISPNVYRTYAEARETFEWFSEVGQWSAYFPEWERNLMVQVGTFAMWLISKRLI
KKHGLSDDVRTHLYDACDKWMGALEKKQTKFMGGNKEPNLADLSVYGVLCSMEGCEAFKD
CLENTKIGLWFYEVKAKVESNRGFRKPTSLYAV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g7713.t1 | CDD | cd03197 | GST_C_mPGES2 | 226 | 380 | 1.58267E-73 |
| 7 | g7713.t1 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 92 | 183 | 2.5E-32 |
| 6 | g7713.t1 | Gene3D | G3DSA:1.20.1050.10 | - | 225 | 383 | 8.7E-60 |
| 2 | g7713.t1 | PANTHER | PTHR12782 | MICROSOMAL PROSTAGLANDIN E SYNTHASE-2 | 90 | 385 | 2.1E-90 |
| 3 | g7713.t1 | PANTHER | PTHR12782:SF5 | PROSTAGLANDIN E SYNTHASE 2 | 90 | 385 | 2.1E-90 |
| 1 | g7713.t1 | Pfam | PF00462 | Glutaredoxin | 105 | 158 | 8.3E-12 |
| 8 | g7713.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 43 | - |
| 10 | g7713.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 44 | 64 | - |
| 9 | g7713.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 65 | 393 | - |
| 13 | g7713.t1 | ProSitePatterns | PS00195 | Glutaredoxin active site. | 107 | 123 | - |
| 14 | g7713.t1 | ProSiteProfiles | PS51354 | Glutaredoxin domain profile. | 93 | 191 | 11.286 |
| 15 | g7713.t1 | SFLD | SFLDG01203 | Prostaglandin E synthase like.1 | 93 | 383 | 0.0 |
| 16 | g7713.t1 | SFLD | SFLDS00019 | Glutathione Transferase (cytosolic) | 93 | 383 | 0.0 |
| 4 | g7713.t1 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 104 | 182 | 3.35E-15 |
| 5 | g7713.t1 | SUPERFAMILY | SSF47616 | GST C-terminal domain-like | 224 | 378 | 3.18E-47 |
| 12 | g7713.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 42 | 64 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0015035 | protein-disulfide reductase activity | MF |
| GO:0009055 | electron transfer activity | MF |
| GO:0050220 | prostaglandin-E synthase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K05309 (microsomal prostaglandin-E synthase 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
