General information for g7731.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as E3 ubiquitin-protein ligase MARCHF6.
Parent gene
- The parent gene g7731 is located on chr_2: 25594152..25594988 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7731 | g7731.t1 | TSS | g7731.t1 | 25591251 | 25591251 |
| chr_2 | g7731 | g7731.t1 | isoform | g7731.t1 | 25591504 | 25594988 |
| chr_2 | g7731 | g7731.t1 | exon | g7731.t1.exon1 | 25591504 | 25591552 |
| chr_2 | g7731 | g7731.t1 | cds | g7731.t1.CDS1 | 25591504 | 25591552 |
| chr_2 | g7731 | g7731.t1 | exon | g7731.t1.exon2 | 25591699 | 25591793 |
| chr_2 | g7731 | g7731.t1 | cds | g7731.t1.CDS2 | 25591699 | 25591793 |
| chr_2 | g7731 | g7731.t1 | exon | g7731.t1.exon3 | 25591893 | 25592043 |
| chr_2 | g7731 | g7731.t1 | cds | g7731.t1.CDS3 | 25591893 | 25592043 |
| chr_2 | g7731 | g7731.t1 | exon | g7731.t1.exon4 | 25592118 | 25592314 |
| chr_2 | g7731 | g7731.t1 | cds | g7731.t1.CDS4 | 25592118 | 25592314 |
| chr_2 | g7731 | g7731.t1 | exon | g7731.t1.exon5 | 25592460 | 25593473 |
| chr_2 | g7731 | g7731.t1 | cds | g7731.t1.CDS5 | 25592460 | 25593473 |
| chr_2 | g7731 | g7731.t1 | exon | g7731.t1.exon6 | 25593543 | 25594988 |
| chr_2 | g7731 | g7731.t1 | cds | g7731.t1.CDS6 | 25593543 | 25594988 |
| chr_2 | g7731 | g7731.t1 | TTS | g7731.t1 | 25595592 | 25595592 |
Sequences
>g7731.t1 Gene=g7731 Length=2952
ATGGAAAGCACACCCGAATGCAGAATATGTCGAACGGAAGCAACAGCAGACCGTCCACTA
TTTTTTCCATGCATATGTACAGGCAGTATCAAATACATTCATCAAGAATGTTTATTGCAA
TGGATGAGATACAGCAGAAAAGAAATATGTGAATTATGTGGGCATCGTTTCAGTTTTACA
CCGATTTATTCTCCAGATATGCCGAAAGTCTTGCCTCTTCGTGTGGTAGCTGGTGGTCTG
TTAAGTTCGATAGCTACAGCTGTGAAATATTGGCTTCATTACACATTAGTTGCTGTTGCA
TGGCTTGGTATTGTCCCTCTCACTGCTTATCGCACATATCGATTTTTCTTTTCCGGATCG
CTTGATATGCTAGTTTCACTTCCATTCGATATGTTTTCAACAGAGAATTTAGCTGCTGAT
GTCTTTCGTGGTTGTTTTGTTGTCACATGTACATTATTTGCTTTCATTGGTTTAGTGTGG
CTAAGAGAGCAGATTCTTCATGGAGGTGGACCAGATTGGTTAGAAAGAGATGAAGGAAAC
AATGTTAATGAAGATATTCCAAGAGCTGCACCACCACCACAAGCAGATAATTTACAGGAA
CCACTTGATGATGAAAATGGCAATAACAATGATGAAATGTTTAATAATAACAACAATAAT
AATGTTTTGGATGCAAACATTCATGGTTTTCAACAGCCACAGCCACAGCAGCCAAATGAT
ATAAATGAGCCGCAACAACCTGCTGCTGCACCAGCAGCTCCAGTACCACAAAACTTTGAA
ATTCCTCAACATAATCCTATAAATAATGCTGAACCACCGCTACCTGATAATGATGAAGAT
CGTAATGAACCTGCAGCTGACAATGCAAATAATGATGATGCTAATGAAGGTATTTGGAAT
CCCATGGAATTTAATAATAATGCAGATTTAGAATTAACGTGGGAAAGATTACTTGGTCTG
GATGGATCAATGGTTTTCCTCGAACATGTCTTCTGGGTAGTGTCTTTAGACACGCTATTC
ATCTTTATTTTCGCATTTTTACCATACACAATTGGAAACTTTGCAATATCATTTCTGGGC
ATAAAACAATTGCACTTTCACGGTCTAGTTACTACACTTTGTGGATATTGCTTAATCGGC
ACTGCATTAGTTAATCTTCATGGAATTGCAAAGTTATTGAAATTGAGAAGACCAAGAAGA
ATTCTTGGATTATGCTACATCGTAGTCAAAGTATCACTCTTATCAGTTGTCGAAATCGGT
GTATTGCCATTGATTTGTGGATGGTGGTTGGATATCTGCAGTTTGCCAATGTTTGATGCT
ACATTAAAAGATCGTAAAGCAAGTTTCAAAACAGCACCAGGAACATCATTGTTTTTACAT
TGGCTTTTCGGGATGATTTATGTATACTACTTTGCATCATTTATTGTGCTTCTTAGAGAA
GTCTTGCGACCTGGCGTTTTGTGGTTCTTAAGAAATCTAAACGATCCAGACTTTTCTCCT
ATTCAGGAAATGATTCATCTATCGATTCTTCGTCATATTCGTCGACTGATTGCATCAGCA
ATAATTTTTGGTTCTGCAGTTTTACTAATGTTATGGCTTCCGATAAAAATATTAAAGGTC
GCATTTCCAACATTCCTACCATACACACTATCGGGAGATTCCGAAGTTAATGAATTAAGT
CTGCAATTACTGCTTCTACAAATCATTCTTCCTGGTTTCTTTGAGCAATCGCAAACACGT
ATATGGTTAAAAGGATTAATACGGATTTGGTGTAATATCGTTTCATGGATATTAGGTATT
AAGAGTTATTTATTGGGAAATGAACCGAGAAACTTTGAACAAGAAGATCGAGCACCACCT
GCTGATCAAGGAGGAGGTTTGGGTGCAGCACATCAAGCACTTTTGCAAAGAGACGTACCA
GTTGGCTTTCAGCCTTATGATAGACCTTCATTCTTTGCAATACGATTGATTGGATTGCTT
ATATTTATGTGTATCAGTTTAGTCATTGGATCTCTGCTTACATTAACTGTTCCAGTTTGG
ATCGGTAGACATGCAATGACAATTTGGTCAATGAGCACGGGAATTGATGCAGGAAAAGTT
GTAAATGTAGAAGCTGGTAGCGGAATTGGAAATAATGCAAATATTGGAGATATTAGTGGA
GATAATGAGACTGTAACATCGACTGTAGTAGCTGTATCAACTTCAAAACCTCATGAACTT
TATACAGCAGCAATTGGCATATACTTGTGTTGGCTCATATCAAAAGGAATAGCATTGGCT
GTAAATTTGTTTCCACAAGGACGCACAGCAATAATTGAGAAATTTAAACATTGGTGTTCA
GTCGCAATTAGTTATGGACTTGCTGCAATAATATTTGTTTTGATGCTTGGTTTAGTTCCA
CTAATGTTCGGTCTTCTGCTTGAACTAGTTGTTGTTGTACCACTGCGAGTACCATTGAAT
CAGACGCCAGTACTCTTTTTGTGGCAAGATTGGGCTTTAGGTTGTCTCTATACAAAAATA
ACAGTCGCACTTTTATTCATGGGTCCAGATTTTCGATTAAAACGCGCCATCGAGCAAGCT
TATCGTGATGGTTTGAGAGAAATGAATTTAAAATTCATCATAAGAGAACTAGCAATACCT
GTGATTTCAGCGTTCGGTTTAGCGTTAGCGGTTCCTTATGTCATCGCACACTCCATCATG
CCAATATTTTTCACAAATCAAATGACTCGTGTCTTAATCTCTCGTCGCATCTATCCATTC
TTCCTTATTGTCATCATTGTCTGTGCAATAATCGTTTTTCAAATTAAACAATTTAAGAAG
CTGTATATTGCCATCAAAAATGATAAATATCTTGTTGGAAAGCGTTTAGTAAATTATGAT
CAGCATCAACGTCAGAAGCTGCTTAATCAACAGCAACAACAACAACAAGCTGAAGTTAGA
GCTGAGCTGTGA
>g7731.t1 Gene=g7731 Length=983
MESTPECRICRTEATADRPLFFPCICTGSIKYIHQECLLQWMRYSRKEICELCGHRFSFT
PIYSPDMPKVLPLRVVAGGLLSSIATAVKYWLHYTLVAVAWLGIVPLTAYRTYRFFFSGS
LDMLVSLPFDMFSTENLAADVFRGCFVVTCTLFAFIGLVWLREQILHGGGPDWLERDEGN
NVNEDIPRAAPPPQADNLQEPLDDENGNNNDEMFNNNNNNNVLDANIHGFQQPQPQQPND
INEPQQPAAAPAAPVPQNFEIPQHNPINNAEPPLPDNDEDRNEPAADNANNDDANEGIWN
PMEFNNNADLELTWERLLGLDGSMVFLEHVFWVVSLDTLFIFIFAFLPYTIGNFAISFLG
IKQLHFHGLVTTLCGYCLIGTALVNLHGIAKLLKLRRPRRILGLCYIVVKVSLLSVVEIG
VLPLICGWWLDICSLPMFDATLKDRKASFKTAPGTSLFLHWLFGMIYVYYFASFIVLLRE
VLRPGVLWFLRNLNDPDFSPIQEMIHLSILRHIRRLIASAIIFGSAVLLMLWLPIKILKV
AFPTFLPYTLSGDSEVNELSLQLLLLQIILPGFFEQSQTRIWLKGLIRIWCNIVSWILGI
KSYLLGNEPRNFEQEDRAPPADQGGGLGAAHQALLQRDVPVGFQPYDRPSFFAIRLIGLL
IFMCISLVIGSLLTLTVPVWIGRHAMTIWSMSTGIDAGKVVNVEAGSGIGNNANIGDISG
DNETVTSTVVAVSTSKPHELYTAAIGIYLCWLISKGIALAVNLFPQGRTAIIEKFKHWCS
VAISYGLAAIIFVLMLGLVPLMFGLLLELVVVVPLRVPLNQTPVLFLWQDWALGCLYTKI
TVALLFMGPDFRLKRAIEQAYRDGLREMNLKFIIRELAIPVISAFGLALAVPYVIAHSIM
PIFFTNQMTRVLISRRIYPFFLIVIIVCAIIVFQIKQFKKLYIAIKNDKYLVGKRLVNYD
QHQRQKLLNQQQQQQQAEVRAEL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 35 | g7731.t1 | CDD | cd16702 | RING_CH-C4HC3_MARCH6 | 6 | 55 | 1.77443E-32 |
| 5 | g7731.t1 | Gene3D | G3DSA:3.30.40.10 | Zinc/RING finger domain | 1 | 65 | 4.0E-20 |
| 2 | g7731.t1 | PANTHER | PTHR13145:SF0 | E3 UBIQUITIN-PROTEIN LIGASE MARCH6 | 4 | 964 | 0.0 |
| 3 | g7731.t1 | PANTHER | PTHR13145 | SSM4 PROTEIN | 4 | 964 | 0.0 |
| 1 | g7731.t1 | Pfam | PF12906 | RING-variant domain | 7 | 53 | 1.9E-14 |
| 9 | g7731.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 90 | - |
| 29 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 91 | 113 | - |
| 20 | g7731.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 114 | 140 | - |
| 24 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 141 | 161 | - |
| 10 | g7731.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 162 | 329 | - |
| 23 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 330 | 352 | - |
| 19 | g7731.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 353 | 363 | - |
| 27 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 364 | 389 | - |
| 13 | g7731.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 390 | 400 | - |
| 22 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 401 | 430 | - |
| 18 | g7731.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 431 | 457 | - |
| 33 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 458 | 478 | - |
| 12 | g7731.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 479 | 515 | - |
| 30 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 516 | 535 | - |
| 16 | g7731.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 536 | 554 | - |
| 21 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 555 | 574 | - |
| 11 | g7731.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 575 | 655 | - |
| 34 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 656 | 681 | - |
| 17 | g7731.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 682 | 739 | - |
| 26 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 740 | 764 | - |
| 8 | g7731.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 765 | 784 | - |
| 25 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 785 | 811 | - |
| 15 | g7731.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 812 | 830 | - |
| 32 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 831 | 851 | - |
| 7 | g7731.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 852 | 871 | - |
| 31 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 872 | 896 | - |
| 14 | g7731.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 897 | 915 | - |
| 28 | g7731.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 916 | 935 | - |
| 6 | g7731.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 936 | 983 | - |
| 51 | g7731.t1 | ProSiteProfiles | PS51292 | Zinc finger RING-CH-type profile. | 1 | 60 | 22.333 |
| 50 | g7731.t1 | SMART | SM00744 | ringv_2 | 6 | 54 | 2.0E-20 |
| 4 | g7731.t1 | SUPERFAMILY | SSF57850 | RING/U-box | 4 | 58 | 5.55E-15 |
| 39 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 91 | 113 | - |
| 49 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 139 | 161 | - |
| 36 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 330 | 352 | - |
| 37 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 367 | 389 | - |
| 48 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 402 | 424 | - |
| 38 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 456 | 478 | - |
| 45 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 516 | 538 | - |
| 41 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 586 | 605 | - |
| 47 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 659 | 681 | - |
| 43 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 742 | 764 | - |
| 40 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 785 | 807 | - |
| 42 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 827 | 849 | - |
| 46 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 877 | 896 | - |
| 44 | g7731.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 911 | 933 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K10661 (E3 ubiquitin-protein ligase MARCH6)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
