General information for g7755.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Mucolipin-3.
Parent gene
- The parent gene g7755 is located on chr_2: 25798764..25801421 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7755 | g7755.t2 | TTS | g7755.t2 | 25797571 | 25797571 |
| chr_2 | g7755 | g7755.t2 | isoform | g7755.t2 | 25798418 | 25800515 |
| chr_2 | g7755 | g7755.t2 | exon | g7755.t2.exon1 | 25798418 | 25799881 |
| chr_2 | g7755 | g7755.t2 | cds | g7755.t2.CDS1 | 25798418 | 25799881 |
| chr_2 | g7755 | g7755.t2 | exon | g7755.t2.exon2 | 25799998 | 25800157 |
| chr_2 | g7755 | g7755.t2 | cds | g7755.t2.CDS2 | 25799998 | 25800157 |
| chr_2 | g7755 | g7755.t2 | exon | g7755.t2.exon3 | 25800215 | 25800515 |
| chr_2 | g7755 | g7755.t2 | cds | g7755.t2.CDS3 | 25800215 | 25800429 |
| chr_2 | g7755 | g7755.t2 | TSS | g7755.t2 | 25800628 | 25800628 |
Sequences
>g7755.t2 Gene=g7755 Length=1925
CCCTAATAGAAGAGAATTCGTGTTGTGAAAGTGATAGTGAGCAAGAGAATCGTGAAAAAT
ATAAAAGTCGCAAAAGAAAAAAAGAAATGAGAAATCACAATAATATACAGCACCAACAAC
AGCAAAGAGAAATTAGTTCAGACGATGAAACTGAATCACAACAATTGAACTATGGAGAAA
CCATTATAAACTCATCAACTCCTATATCATCTTCAATGTCTTTATATAATCAAGAAAAAA
TGAGAAGACGATTACAATTTCATTTTATGAATCCTATAGAAAAATGGCAAGCTAAACGGA
GATTTCCTTACAAATTCGTAGTTCAGGTAATAAAAATTGTTTTAGTAACAATTCAACTAT
GTTTATTCGCACATTCACGCTACATTCATGTAAATTATACTTGGGATAATAGAGTGACAT
TTAGTCACTTATTTTTGAAGAACTGGACACCCGAAATGGAGGTGAGCACTTACCCACCCG
AAGTGGGACCACTCTCTGTTTATACGGTCGATGAATTTTGGAATACAATTGATTATGCAA
TTTATGGTTTTGGTAATCTAAGTCAAGCCATCGGACCATACTCGTACGCAACTGATGATA
ACACTATGGGACCAGTTGAATTATGCTTTTATCGCTATAAAGAAGGCACTGTATTTGGCT
TCAATGAAAGTTATATATTTAACCCTGAGATAGATACAATGTGTTTAAGAGTACATGGAA
ATGATACGCGCGGTGGAATTCAAGAGTATTTACAAAAACAAAACATTACCATTGAATTCT
CAGCACTCGTGAAAGCCACAATCCATTTCAATATCAAAACTGTGAATTTAAAAGCAAGTG
GCCCAATTTCTGCACCTGATTGCTATAAATTCAAAGTTTTTATTACATTTGATAACACTG
ACCACGATGGTCAAATGATTCTACAATTAGACGCCATTCCTGTGAGATTAGTTTGCAGAG
GAGATACGCAATTTATTACGAATAGTAAAATTGATGCAGCGCTTCGATCAGTTTTGAACG
TGTTTGTCATCATAATTTGTGCAATTTCGCTTGCACTATGCAGTCGTGCAATCTTTCGTG
CACAACTGTTGAGAATTAAAACTGATGAATTTTTCATTCAAGTATTTGGACGTGAGTTGA
GTTCAAAAGGTCGATGGGAATTTGTAAATATGTGGTATATTATGATTTGCGTTAATGACG
TATTATTAATCCTCGGTTCAGCTATAAAGGAGCAAATTGAACGAAAACATTTTGAGGCAG
ATGAGTGGAACAATTGTTCATTATTGCTTGGTATAGGCAATCTACTTGTATGGTTTGGCG
TGCTGCGTTATCTTGGATTTTTCAGAACATACAACGTCGTTATTTTGACACTACAAAAGG
CGCTGCCACATATCGGACGGTTCCTATTATGTGCTCTTCTTATCTATGCGGGTTTCACAT
TCTGCGGGTGGCTGATTCTCGGTCCCTATCACATAAAATTCCGATCACTTTCAACAACTT
CTGAATGTCTCTTTGCATTGATCAATGGTGATGATATGTTTGCGACGTTCAGCATAATGT
CAGATAAATCAATGACGCTCTGGTGGTTCTGTCGTATCTACTTGTATGTATTTATTAGTT
TGTACATTTACGTCGTACTCTCGCTCTTCATATCAGTTATTATGGATTCATACGATACAA
TCAAAAAATATTATACCGACGGCTTCCCACAAAGTGATTTACGCCAATTTATGGGTGAAG
CACACATTAGTGATCTGTCATCTGGTGTATATCGAGATGAAGTTGATGATGATGAAGCTA
TTGGTGAGAGTTTAAGGAAAATATTTTGTTTTTGGAAGAGACGTGGAAGTAATGATACTT
CAGGTTCAACTACTGCATATACAACTTTAACGCCAAAGCCATCAGTTTCATCTGCAACCT
ATTGA
>g7755.t2 Gene=g7755 Length=612
MRNHNNIQHQQQQREISSDDETESQQLNYGETIINSSTPISSSMSLYNQEKMRRRLQFHF
MNPIEKWQAKRRFPYKFVVQVIKIVLVTIQLCLFAHSRYIHVNYTWDNRVTFSHLFLKNW
TPEMEVSTYPPEVGPLSVYTVDEFWNTIDYAIYGFGNLSQAIGPYSYATDDNTMGPVELC
FYRYKEGTVFGFNESYIFNPEIDTMCLRVHGNDTRGGIQEYLQKQNITIEFSALVKATIH
FNIKTVNLKASGPISAPDCYKFKVFITFDNTDHDGQMILQLDAIPVRLVCRGDTQFITNS
KIDAALRSVLNVFVIIICAISLALCSRAIFRAQLLRIKTDEFFIQVFGRELSSKGRWEFV
NMWYIMICVNDVLLILGSAIKEQIERKHFEADEWNNCSLLLGIGNLLVWFGVLRYLGFFR
TYNVVILTLQKALPHIGRFLLCALLIYAGFTFCGWLILGPYHIKFRSLSTTSECLFALIN
GDDMFATFSIMSDKSMTLWWFCRIYLYVFISLYIYVVLSLFISVIMDSYDTIKKYYTDGF
PQSDLRQFMGEAHISDLSSGVYRDEVDDDEAIGESLRKIFCFWKRRGSNDTSGSTTAYTT
LTPKPSVSSATY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g7755.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 22 | - |
| 2 | g7755.t2 | PANTHER | PTHR12127 | MUCOLIPIN | 39 | 565 | 5.9E-163 |
| 1 | g7755.t2 | Pfam | PF08016 | Polycystin cation channel | 401 | 532 | 3.4E-9 |
| 11 | g7755.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 76 | - |
| 18 | g7755.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 77 | 96 | - |
| 14 | g7755.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 97 | 308 | - |
| 17 | g7755.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 309 | 330 | - |
| 9 | g7755.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 331 | 358 | - |
| 16 | g7755.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 359 | 379 | - |
| 15 | g7755.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 380 | 398 | - |
| 20 | g7755.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 399 | 418 | - |
| 10 | g7755.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 419 | 438 | - |
| 21 | g7755.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 439 | 458 | - |
| 13 | g7755.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 459 | 503 | - |
| 19 | g7755.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 504 | 526 | - |
| 12 | g7755.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 527 | 612 | - |
| 5 | g7755.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 308 | 330 | - |
| 3 | g7755.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 359 | 381 | - |
| 7 | g7755.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 394 | 416 | - |
| 6 | g7755.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 436 | 458 | - |
| 4 | g7755.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 504 | 526 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005261 | cation channel activity | MF |
KEGG
Orthology
- This transcript has been annotated with K04994 (mucolipin 3)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
