General information for g7778.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Ecdysone-induced protein 74EF isoform B.
Parent gene
- The parent gene g7778 is located on chr_2: 25972767..25975438 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7778 | g7778.t1 | isoform | g7778.t1 | 25934277 | 25972767 |
| chr_2 | g7778 | g7778.t1 | exon | g7778.t1.exon1 | 25934277 | 25935013 |
| chr_2 | g7778 | g7778.t1 | cds | g7778.t1.CDS1 | 25934277 | 25935013 |
| chr_2 | g7778 | g7778.t1 | exon | g7778.t1.exon2 | 25946123 | 25946377 |
| chr_2 | g7778 | g7778.t1 | cds | g7778.t1.CDS2 | 25946123 | 25946377 |
| chr_2 | g7778 | g7778.t1 | exon | g7778.t1.exon3 | 25953357 | 25953599 |
| chr_2 | g7778 | g7778.t1 | cds | g7778.t1.CDS3 | 25953357 | 25953599 |
| chr_2 | g7778 | g7778.t1 | TTS | g7778.t1 | 25971992 | 25971992 |
| chr_2 | g7778 | g7778.t1 | exon | g7778.t1.exon4 | 25972149 | 25972767 |
| chr_2 | g7778 | g7778.t1 | cds | g7778.t1.CDS4 | 25972149 | 25972767 |
| chr_2 | g7778 | g7778.t1 | TSS | g7778.t1 | 25973835 | 25973835 |
Sequences
>g7778.t1 Gene=g7778 Length=1854
ATGTTGGCATATCAACCATCAATTTCGCATCATTTTGCCGGCAGTCCGCAAAAAGAAAAT
CACGAAGAATCTCAAAAAATTCGATCAATTAGTCCTGGTGAGCTTGCATATCGCCGTGAA
GCATTAGATTATGTTAAACACATTAAACGAAGTCTCTCACGTTCATCATCACCAATTAGA
TCATATTCACCAACATATGCAATGAGCCATACAAGAAATGGACAAGAACATTTTCTAATT
AAACGTTCATTGAGCGAGGAAGACTTACGTGCAACACATGACCGTAGAAGGCTGTCATTA
GAGCAATCTGAACACCATCATCATGCCATAACACATAGGCCACAAATTTTGATAAATTAT
CATCATCATCGAGTAATGCAAGCAACTGATGACGATGCTGATTCAGATGGCGAAAACAAC
AATAATAATACGAAAATTAATGATGAAAATTTAAAAGAAAATCATGATGATGATGCACCA
TTGGATTTATCGATGGGTTCAAAGAAACGACGAGGCCGTGCTGATTCGGGCACAGATTCT
GATGATTCAACTGGCATGGGTGACGAAGGACGTGGTAATGAAGGAAGAGCATATAAGAAA
AGTCTCATGAAACGCTACTATACTGAAATTCCATGTCAGCTACCGCAATCACATTCACCA
AGCCCGCCACCGCCTCAACAGATTGCTCATCAACAGGCTCATAGAATTGTGCAAGAATTA
CAGCAGCAGCAGCAACAACAACAACCACATCCGTTACAAAATGGTCCACCGCCCCAACAT
CGACCCCTGCTGCATGGTCTCTTAAGTGGTACGCACATTTCACAAGCGTCATATCATCGA
GGTTATAGCTCATCGAGCACAGGATCACTGCCACCATCACCTGCCGATAGTGGTGTATCG
GATGTAGATAGCTCAAGTAGCGGCAATGGACAACCCATTTGCAGTGATGAGATCAAAGCA
CGTTTAGGTTTAAATGGAACAACATCAACACCAAATCAAACAGGACAACAACAACAGCAA
CAACAATTGCCACCACCACCGCCCGGAACATTCTTACATCCTAACTTTTATTCACAAGCA
CCATTGCGAAATTTATGGAATAATAGGAGTGTTTCATTACCAGACAATTACTACCTGCAC
AATCTCAGTAATAGCAACAGCACCGCCAACGGTTACACGTCAAATTTCACATCACCGTCA
CCACCACGCACGACGCTCAATTTGACGGGTGCCTCAAATACAAATTCGTCCACTGCCAAT
GGTCTAACGCATCACTCCACCGGTCTGCATCCGCAGAGCTTTCATCCACATCATTCAGCC
GCTGCCGTAGCCGCAGCTGCAGCTCATCATCATCATCAACAAAGTGTGATTCAAGCAGCA
ACATCAAGTTGTCAAGATGACCTCTCATACATGCTCGAGTATGGATTTAATCCACAGTCA
GTTCGTAATGGTATGAATGGTCTTGGTCCACACAGTCATCACAGTCTCAATAAACTGAAG
AAGCCTAAAAAACCAAAACTCGAGATGGGAATTAAAAGAAAGAGTCGCGAAGGATCGACG
ACATATTTATGGGAATTTTTACTGAAACTTCTTCAAGATCGCGAATATTGTCCGAGATTC
ATTAAATGGACAAATCGTGATAAGGGAATCTTTAAACTTGTCGATTCTAAAGCTGTGTCG
AAATTATGGGGAATGCATAAGAACAAGCCTGATATGAATTATGAAACAATGGGTCGAGCT
CTTCGATATTATTATCAACGTGGCATTCTTGCAAAAGTTGATGGTCAGCGGCTAGTGTAT
CAGTTTGTTGAAGTACCAAAAGACATAATAGAGATCGATTGCACAGGACAATGA
>g7778.t1 Gene=g7778 Length=617
MLAYQPSISHHFAGSPQKENHEESQKIRSISPGELAYRREALDYVKHIKRSLSRSSSPIR
SYSPTYAMSHTRNGQEHFLIKRSLSEEDLRATHDRRRLSLEQSEHHHHAITHRPQILINY
HHHRVMQATDDDADSDGENNNNNTKINDENLKENHDDDAPLDLSMGSKKRRGRADSGTDS
DDSTGMGDEGRGNEGRAYKKSLMKRYYTEIPCQLPQSHSPSPPPPQQIAHQQAHRIVQEL
QQQQQQQQPHPLQNGPPPQHRPLLHGLLSGTHISQASYHRGYSSSSTGSLPPSPADSGVS
DVDSSSSGNGQPICSDEIKARLGLNGTTSTPNQTGQQQQQQQLPPPPPGTFLHPNFYSQA
PLRNLWNNRSVSLPDNYYLHNLSNSNSTANGYTSNFTSPSPPRTTLNLTGASNTNSSTAN
GLTHHSTGLHPQSFHPHHSAAAVAAAAAHHHHQQSVIQAATSSCQDDLSYMLEYGFNPQS
VRNGMNGLGPHSHHSLNKLKKPKKPKLEMGIKRKSREGSTTYLWEFLLKLLQDREYCPRF
IKWTNRDKGIFKLVDSKAVSKLWGMHKNKPDMNYETMGRALRYYYQRGILAKVDGQRLVY
QFVEVPKDIIEIDCTGQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g7778.t1 | Gene3D | G3DSA:1.10.10.10 | winged helix repressor DNA binding domain | 489 | 615 | 2.6E-42 |
| 18 | g7778.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 28 | - |
| 13 | g7778.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 130 | 195 | - |
| 16 | g7778.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 148 | 195 | - |
| 14 | g7778.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 214 | 255 | - |
| 17 | g7778.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 231 | 250 | - |
| 15 | g7778.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 278 | 312 | - |
| 2 | g7778.t1 | PANTHER | PTHR11849 | ETS | 197 | 613 | 4.6E-87 |
| 3 | g7778.t1 | PANTHER | PTHR11849:SF191 | ECDYSONE-INDUCED PROTEIN 74EF ISOFORM B | 197 | 613 | 4.6E-87 |
| 7 | g7778.t1 | PRINTS | PR00454 | ETS domain signature | 521 | 534 | 9.7E-26 |
| 5 | g7778.t1 | PRINTS | PR00454 | ETS domain signature | 547 | 565 | 9.7E-26 |
| 6 | g7778.t1 | PRINTS | PR00454 | ETS domain signature | 566 | 584 | 9.7E-26 |
| 4 | g7778.t1 | PRINTS | PR00454 | ETS domain signature | 585 | 603 | 9.7E-26 |
| 1 | g7778.t1 | Pfam | PF00178 | Ets-domain | 523 | 603 | 3.9E-34 |
| 10 | g7778.t1 | ProSitePatterns | PS00345 | Ets-domain signature 1. | 523 | 531 | - |
| 11 | g7778.t1 | ProSitePatterns | PS00346 | Ets-domain signature 2. | 569 | 584 | - |
| 19 | g7778.t1 | ProSiteProfiles | PS50061 | Ets-domain profile. | 521 | 603 | 26.778 |
| 12 | g7778.t1 | SMART | SM00413 | etsneu2 | 520 | 607 | 9.5E-53 |
| 8 | g7778.t1 | SUPERFAMILY | SSF46785 | Winged helix DNA-binding domain | 519 | 610 | 5.14E-41 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006355 | regulation of transcription, DNA-templated | BP |
| GO:0043565 | sequence-specific DNA binding | MF |
| GO:0003700 | DNA-binding transcription factor activity | MF |
KEGG
Orthology
- This transcript has been annotated with K09428 (E74-like factor 1/2/4)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
