General information for g7854.t8
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Frizzled.
Parent gene
- The parent gene g7854 is located on chr_2: 26423754..26424291 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7854 | g7854.t8 | isoform | g7854.t8 | 26422968 | 26424291 |
| chr_2 | g7854 | g7854.t8 | exon | g7854.t8.exon1 | 26422968 | 26423157 |
| chr_2 | g7854 | g7854.t8 | cds | g7854.t8.CDS1 | 26422969 | 26423157 |
| chr_2 | g7854 | g7854.t8 | exon | g7854.t8.exon2 | 26423215 | 26423375 |
| chr_2 | g7854 | g7854.t8 | cds | g7854.t8.CDS2 | 26423215 | 26423375 |
| chr_2 | g7854 | g7854.t8 | exon | g7854.t8.exon3 | 26423441 | 26423684 |
| chr_2 | g7854 | g7854.t8 | cds | g7854.t8.CDS3 | 26423441 | 26423684 |
| chr_2 | g7854 | g7854.t8 | exon | g7854.t8.exon4 | 26423750 | 26424291 |
| chr_2 | g7854 | g7854.t8 | cds | g7854.t8.CDS4 | 26423750 | 26423764 |
| chr_2 | g7854 | g7854.t8 | TSS | g7854.t8 | 26424417 | 26424417 |
| chr_2 | g7854 | g7854.t8 | TTS | g7854.t8 | NA | NA |
Sequences
>g7854.t8 Gene=g7854 Length=1137
ATGTCGAATAATGTAAAGTTTAGTTTGTTAGTTTTATTTCTTAAAGTATTTTTTGTAATA
TCTCAACAATCGAGCGTAGTAATATCAACAGAGTCAAAAACTCGACAGAAGGATTACGAT
CGAATATCAAGTTATAATCAAAAATGCGAGCCAATACAAATTCCGCTTTGCCAAGATATA
CAATTTTATAATCAAACAATTTTTCCAAATCTCCTGAATCATGCACGACAGGTAAATAAA
ATATTTTTGTACTTATTATGTGATGGAATTCCATAAATGGTGACATGATGAACTCAATTT
CCATTTTTTTTATTTATTTTCTCTTAATTGCACTCAATTAATATTGCAATCGATAGGACG
AAGCAGCGCTTGAAGTTCATCAATTTATTCCACTTATTAAAATAAATTGTTCTCCCGACC
TGAAATTATTTTTATGCTCACTTTATGCTCCGATATGTACAATCTTGGATTATCCTATTC
CGCCATGCAGAAAGTTATGTGAAAGTGCCAGAAATTGTGAAAAAATTATGAGAACATTTG
ACTTTCAATGGCCCGAGAATCTCGAATGCTCGAAATTCCCTGAAGACGGTACAAAAGATA
TTTGCATATCACCCAACAACTCAGCACCGTCATCTTCTTCATCACCATCACAAGATTCAC
ATCAATTCAGTACACAAATTACATCGCTTGGAAAAGTAGAGCGAAAAAATCAGCATGACT
CGCGAAACGCAACATCTTTTTCACAGCATAAATTTAGAGGATTTATTTGTCCAGTGCAAT
TGAAAGCACCTGCCGCACTGGGATATGAGCTAAATATCGGTGGAAAAGTTGTAAAAGATT
GTGGAGCTCCATGCAGCTCTCTATTTTTTGATGACAAAGAAGTCACAATGCTTAGATATT
GGATAAGTTTTTGGGCAATTATATGTGTGGCTTGTTGCTTATTTACTATTCTAACATTTA
TTATCGATTCATCGCGATTTCGATATCCAGAACGTCCTATCGTATTTATTGCGATATGCT
ATTTAATAGTTGCGACTGCTTATATTTCGGGATTGAGTGCTGGAAAAAATATTTCATGCC
GTGATCCATTTGTGCATACAGTGAAGTTGGGACGCTTGCAAATGATTTCAACAATTA
>g7854.t8 Gene=g7854 Length=203
MRTFDFQWPENLECSKFPEDGTKDICISPNNSAPSSSSSPSQDSHQFSTQITSLGKVERK
NQHDSRNATSFSQHKFRGFICPVQLKAPAALGYELNIGGKVVKDCGAPCSSLFFDDKEVT
MLRYWISFWAIICVACCLFTILTFIIDSSRFRYPERPIVFIAICYLIVATAYISGLSAGK
NISCRDPFVHTVKLGRLQMISTI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g7854.t8 | Gene3D | G3DSA:1.20.1070.10 | - | 88 | 200 | 1.0E-31 |
| 2 | g7854.t8 | PANTHER | PTHR11309 | FRIZZLED | 1 | 188 | 1.1E-38 |
| 3 | g7854.t8 | PANTHER | PTHR11309:SF31 | FRIZZLED-7 | 1 | 188 | 1.1E-38 |
| 4 | g7854.t8 | PRINTS | PR00489 | Frizzled protein signature | 125 | 147 | 2.3E-18 |
| 5 | g7854.t8 | PRINTS | PR00489 | Frizzled protein signature | 153 | 175 | 2.3E-18 |
| 1 | g7854.t8 | Pfam | PF01534 | Frizzled/Smoothened family membrane region | 113 | 190 | 5.2E-26 |
| 11 | g7854.t8 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 123 | - |
| 14 | g7854.t8 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 124 | 146 | - |
| 10 | g7854.t8 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 147 | 157 | - |
| 13 | g7854.t8 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 158 | 178 | - |
| 12 | g7854.t8 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 179 | 203 | - |
| 8 | g7854.t8 | SMART | SM01330 | Frizzled_2 | 112 | 202 | 4.3E-4 |
| 7 | g7854.t8 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 124 | 146 | - |
| 6 | g7854.t8 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 156 | 178 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0042813 | Wnt-activated receptor activity | MF |
| GO:0016055 | Wnt signaling pathway | BP |
| GO:0007166 | cell surface receptor signaling pathway | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
