General information for g7951.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 26S proteasome regulatory subunit 8.
Parent gene
- The parent gene g7951 is located on chr_2: 27180804..27181540 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7951 | g7951.t1 | TSS | g7951.t1 | 27179950 | 27179950 |
| chr_2 | g7951 | g7951.t1 | isoform | g7951.t1 | 27180053 | 27181540 |
| chr_2 | g7951 | g7951.t1 | exon | g7951.t1.exon1 | 27180053 | 27180070 |
| chr_2 | g7951 | g7951.t1 | cds | g7951.t1.CDS1 | 27180053 | 27180070 |
| chr_2 | g7951 | g7951.t1 | exon | g7951.t1.exon2 | 27180138 | 27180276 |
| chr_2 | g7951 | g7951.t1 | cds | g7951.t1.CDS2 | 27180138 | 27180276 |
| chr_2 | g7951 | g7951.t1 | exon | g7951.t1.exon3 | 27180336 | 27180738 |
| chr_2 | g7951 | g7951.t1 | cds | g7951.t1.CDS3 | 27180336 | 27180738 |
| chr_2 | g7951 | g7951.t1 | exon | g7951.t1.exon4 | 27180800 | 27181176 |
| chr_2 | g7951 | g7951.t1 | cds | g7951.t1.CDS4 | 27180800 | 27181176 |
| chr_2 | g7951 | g7951.t1 | exon | g7951.t1.exon5 | 27181266 | 27181540 |
| chr_2 | g7951 | g7951.t1 | cds | g7951.t1.CDS5 | 27181266 | 27181540 |
| chr_2 | g7951 | g7951.t1 | TTS | g7951.t1 | 27181627 | 27181627 |
Sequences
>g7951.t1 Gene=g7951 Length=1212
ATGACGGCCTCGTCTAAGATGGAAATTGATCAACAGCGAGGCGAAGGTTTTCGCAATTAT
TACATTCAAAAAATTGAAGAATTACAGTTGATAGTTAATGAAAAGAGTCAGAACCTTCGT
CGTCTTCAAGCACAACGTAATGAGTTGAATGCAAAAGTTCGAATGTTGCGTGAAGAACTG
CAATTGCTGCAAGAACAAGGAAGTTATGTTGGAGAAGTAGTGAAACCAATGGATAAGAAG
AAAGTATTAGTAAAAGTTCATCCAGAGGGAAAGTTTGTTGTCGATTTAGACAAGAACATT
GACATCAATGATGTTACTCCAAATTCTCGGGTTGCTCTAAGAAATGAGAGTTACACTTTG
CACAAGATTCTACCAAATAAGGTTGATCCATTAGTTTCACTCATGATGGTCGAGAAAGTT
CCGGACTCAACTTATGAAATGGTTGGTGGTTTAGATAAACAAATTAAGGAAATTAAAGAA
GTCATTGAATTGCCAGTAAAACATCCAGAATTATTTGATGCTCTTGGTATTGCTCAACCA
AAAGGTGTCTTATTATATGGTCCGCCAGGTACTGGTAAAACTCTTCTCGCTCGTGCGGTT
GCTCATCACACAGAATGTACTTTCATTCGTGTTTCTGGATCTGAACTTGTTCAGAAGTTT
ATAGGAGAGGGCTCACGTATGGTTCGAGAATTATTCGTAATGGCAAGAGAACATGCACCT
TCCATCATTTTTATGGATGAAATTGATTCTATCGGTTCATCTCGTATTGAATCTGGCAGC
GGTGGTGATTCTGAAGTACAACGAACAATGTTGGAATTACTTAATCAGCTTGATGGTTTC
GAAGCAACTAAAAATATTAAGGTCATCATGGCAACGAATCGTATTGACATTTTAGATCAG
GCGCTTTTGAGACCCGGAAGAATTGATAGAAAGATTGAATTTCCTCCACCTAATGAGGAA
GCTCGAGGAGATATTTTAAAGATTCATTCCCGCAAGATGAATTTGACCCGCGGCATTAAT
TTAAAAAAGATAGCCGAGTTGATGCCAGGTGCTTCGGGCGCAGAAGTTAAAGGTGTTTGT
ACTGAAGCTGGCATGTACGCTTTGCGTGAACGTCGTGTTCATGTTACACAAGAAGACTTT
GAGATGGCTGTTGCCAAAATTATGCAAAAAGATTCCGAGAAGAATATGTCGATTAAAAAG
TTGTGGAAGTAA
>g7951.t1 Gene=g7951 Length=403
MTASSKMEIDQQRGEGFRNYYIQKIEELQLIVNEKSQNLRRLQAQRNELNAKVRMLREEL
QLLQEQGSYVGEVVKPMDKKKVLVKVHPEGKFVVDLDKNIDINDVTPNSRVALRNESYTL
HKILPNKVDPLVSLMMVEKVPDSTYEMVGGLDKQIKEIKEVIELPVKHPELFDALGIAQP
KGVLLYGPPGTGKTLLARAVAHHTECTFIRVSGSELVQKFIGEGSRMVRELFVMAREHAP
SIIFMDEIDSIGSSRIESGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDQ
ALLRPGRIDRKIEFPPPNEEARGDILKIHSRKMNLTRGINLKKIAELMPGASGAEVKGVC
TEAGMYALRERRVHVTQEDFEMAVAKIMQKDSEKNMSIKKLWK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g7951.t1 | CDD | cd00009 | AAA | 149 | 316 | 1.92659E-28 |
| 10 | g7951.t1 | Coils | Coil | Coil | 25 | 66 | - |
| 9 | g7951.t1 | Gene3D | G3DSA:2.40.50.140 | - | 32 | 135 | 2.4E-37 |
| 7 | g7951.t1 | Gene3D | G3DSA:3.40.50.300 | - | 136 | 316 | 6.0E-69 |
| 8 | g7951.t1 | Gene3D | G3DSA:1.10.8.60 | - | 317 | 389 | 9.1E-25 |
| 4 | g7951.t1 | PANTHER | PTHR23073 | 26S PROTEASOME REGULATORY SUBUNIT | 12 | 400 | 2.0E-263 |
| 5 | g7951.t1 | PANTHER | PTHR23073:SF102 | BNAA02G04630D PROTEIN | 12 | 400 | 2.0E-263 |
| 1 | g7951.t1 | Pfam | PF16450 | Proteasomal ATPase OB C-terminal domain | 70 | 125 | 2.4E-8 |
| 2 | g7951.t1 | Pfam | PF00004 | ATPase family associated with various cellular activities (AAA) | 183 | 315 | 1.2E-42 |
| 3 | g7951.t1 | Pfam | PF17862 | AAA+ lid domain | 339 | 381 | 8.1E-13 |
| 12 | g7951.t1 | ProSitePatterns | PS00674 | AAA-protein family signature. | 286 | 304 | - |
| 13 | g7951.t1 | SMART | SM00382 | AAA_5 | 179 | 318 | 3.4E-23 |
| 6 | g7951.t1 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 136 | 386 | 4.08E-71 |
| 14 | g7951.t1 | TIGRFAM | TIGR01242 | 26Sp45: 26S proteasome subunit P45 family | 31 | 388 | 7.6E-138 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0036402 | proteasome-activating activity | MF |
| GO:0030163 | protein catabolic process | BP |
| GO:0005737 | cytoplasm | CC |
| GO:0016887 | ATP hydrolysis activity | MF |
KEGG
Orthology
- This transcript has been annotated with K03066 (26S proteasome regulatory subunit T6)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
