General information for g7986.t13
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as NAD kinase.
Parent gene
- The parent gene g7986 is located on chr_2: 27480927..27481830 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7986 | g7986.t13 | TTS | g7986.t13 | 27475837 | 27475837 |
| chr_2 | g7986 | g7986.t13 | isoform | g7986.t13 | 27476061 | 27477662 |
| chr_2 | g7986 | g7986.t13 | exon | g7986.t13.exon1 | 27476061 | 27476256 |
| chr_2 | g7986 | g7986.t13 | cds | g7986.t13.CDS1 | 27476177 | 27476256 |
| chr_2 | g7986 | g7986.t13 | exon | g7986.t13.exon2 | 27476328 | 27476537 |
| chr_2 | g7986 | g7986.t13 | cds | g7986.t13.CDS2 | 27476328 | 27476537 |
| chr_2 | g7986 | g7986.t13 | exon | g7986.t13.exon3 | 27476598 | 27476709 |
| chr_2 | g7986 | g7986.t13 | cds | g7986.t13.CDS3 | 27476598 | 27476709 |
| chr_2 | g7986 | g7986.t13 | exon | g7986.t13.exon4 | 27476831 | 27477212 |
| chr_2 | g7986 | g7986.t13 | cds | g7986.t13.CDS4 | 27476831 | 27477212 |
| chr_2 | g7986 | g7986.t13 | exon | g7986.t13.exon5 | 27477270 | 27477493 |
| chr_2 | g7986 | g7986.t13 | cds | g7986.t13.CDS5 | 27477270 | 27477493 |
| chr_2 | g7986 | g7986.t13 | exon | g7986.t13.exon6 | 27477560 | 27477662 |
| chr_2 | g7986 | g7986.t13 | cds | g7986.t13.CDS6 | 27477560 | 27477592 |
| chr_2 | g7986 | g7986.t13 | TSS | g7986.t13 | 27477907 | 27477907 |
Sequences
>g7986.t13 Gene=g7986 Length=1227
CCATTTAAGGCGAACGCGAAGCTTAAATGCGCCCAGCCCAGTGCAGCAATTTGGTCCATG
CGGACGAATTATGAAAAACTCTGCAATGGTCATGCAAATTCAAGACCCTGCTTCGCAACG
CTTAACATGGTACAAACCACCACTAGCTGTGTTAGTTGTTAAGAAAGTTCGAGATTCAAC
TGTTCTATCGCCATTTGTACAATTAGTTGAATGGTTGATACAAGAAAAACATATGGTTGT
GTGGGTTGAATCAGCTGTTCTCGAAGATCCATTATTAATGCAAGATAAAAAGTTTCTCAA
TATTAAAGATAAATTAGTAACATTCAAGGACGGAAGAGACGATCTTACAGACCGAATTGA
TTTCATAATTTGCCTTGGAGGAGACGGAACATTGCTGTATGCATCGTTATTGTTTCAACA
ATCTGTACCTCCGGTGATGGCTTTTCATTTGGGCTCGTTGGGCTTCTTGACACCATTCCA
ATTTCAAAACTTTCAAGAGCAAGTGACAAATGTCCTCGAGGGACACGCTGCTTTAACATT
ACGTAGCAGACTTCGTTGCATCATCGTGCGTAAAAGTATCAAAGATCATAAGAGTGATTC
AGATCGAAATGACAGCAGCTCAGCTGAGTATGACTGTAGTGGTTCAAATAGTATTAGTAG
TAGCAATTGCAATACATTGCCTTCAACAAATATTCTCGTGCTCAATGAGGTCGTTATCGA
TCGTGGCCCATCTCCATATTTAAGTAATATTGATTTGTTTTTGGATGGACGACACATTAC
TTCAGTTCAGGGCGATGGCCTTATAATATCTTCACCAAGTGGAAGTACGGCTTATGCACT
GGCAGCAGGTGCTTCTATGATTCATCCGTCAGTTCCCGCAATAATGGTTACACCAATTTG
TCCACATTCATTAAGTTTTCGGCCAATAGTCGTTCCAGCTGGTGTTGAACTTAAAATTTC
TGTATCTCCTGATAGTCGCAATACATCTTGGGTTTCATTTGATGGGCGAAATCGTCAAGA
ATTATTACATGTTTACGTGTTACAACTTCGATATATCCTGTTCCTAGTATTTGCTCTCAG
GATCAAATCTCAGATTGGTTCGACTCATTAGCAGAATGTTTGCATTGGAATGTTCGAAAG
CGCCAAAAATGTTTAGATGAGCTGAGCGATTTGACAGGAAGTGGTAGTGAAGATACTCTA
GACGAAATAGACAATGTTCAAATTTGA
>g7986.t13 Gene=g7986 Length=346
MKNSAMVMQIQDPASQRLTWYKPPLAVLVVKKVRDSTVLSPFVQLVEWLIQEKHMVVWVE
SAVLEDPLLMQDKKFLNIKDKLVTFKDGRDDLTDRIDFIICLGGDGTLLYASLLFQQSVP
PVMAFHLGSLGFLTPFQFQNFQEQVTNVLEGHAALTLRSRLRCIIVRKSIKDHKSDSDRN
DSSSAEYDCSGSNSISSSNCNTLPSTNILVLNEVVIDRGPSPYLSNIDLFLDGRHITSVQ
GDGLIISSPSGSTAYALAAGASMIHPSVPAIMVTPICPHSLSFRPIVVPAGVELKISVSP
DSRNTSWVSFDGRNRQELLHVYVLQLRYILFLVFALRIKSQIGSTH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g7986.t13 | Gene3D | G3DSA:3.40.50.12540 | - | 1 | 190 | 2.1E-56 |
| 7 | g7986.t13 | Gene3D | G3DSA:2.60.200.30 | - | 194 | 332 | 5.8E-49 |
| 4 | g7986.t13 | Hamap | MF_00361 | NAD kinase [nadK]. | 25 | 344 | 23.117809 |
| 2 | g7986.t13 | PANTHER | PTHR20275:SF29 | NAD KINASE B | 2 | 325 | 1.4E-141 |
| 3 | g7986.t13 | PANTHER | PTHR20275 | NAD KINASE | 2 | 325 | 1.4E-141 |
| 1 | g7986.t13 | Pfam | PF01513 | ATP-NAD kinase | 32 | 318 | 1.6E-58 |
| 9 | g7986.t13 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 318 | - |
| 10 | g7986.t13 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 319 | 338 | - |
| 8 | g7986.t13 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 339 | 346 | - |
| 5 | g7986.t13 | SUPERFAMILY | SSF111331 | NAD kinase/diacylglycerol kinase-like | 27 | 333 | 1.83E-76 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0003951 | NAD+ kinase activity | MF |
| GO:0006741 | NADP biosynthetic process | BP |
| GO:0019674 | NAD metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K00858 (NAD+ kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
