General information for g8077.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Ceramide glucosyltransferase.
Parent gene
- The parent gene g8077 is located on chr_2: 28488535..28488889 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8077 | g8077.t1 | TSS | g8077.t1 | 28483392 | 28483392 |
| chr_2 | g8077 | g8077.t1 | isoform | g8077.t1 | 28483796 | 28488889 |
| chr_2 | g8077 | g8077.t1 | exon | g8077.t1.exon1 | 28483796 | 28483890 |
| chr_2 | g8077 | g8077.t1 | cds | g8077.t1.CDS1 | 28483796 | 28483890 |
| chr_2 | g8077 | g8077.t1 | exon | g8077.t1.exon2 | 28487534 | 28488316 |
| chr_2 | g8077 | g8077.t1 | cds | g8077.t1.CDS2 | 28487534 | 28488316 |
| chr_2 | g8077 | g8077.t1 | exon | g8077.t1.exon3 | 28488521 | 28488653 |
| chr_2 | g8077 | g8077.t1 | cds | g8077.t1.CDS3 | 28488521 | 28488653 |
| chr_2 | g8077 | g8077.t1 | exon | g8077.t1.exon4 | 28488710 | 28488889 |
| chr_2 | g8077 | g8077.t1 | cds | g8077.t1.CDS4 | 28488710 | 28488889 |
| chr_2 | g8077 | g8077.t1 | TTS | g8077.t1 | 28489613 | 28489613 |
Sequences
>g8077.t1 Gene=g8077 Length=1191
ATGTCATTTTCCATTTATATTTATGGTTTCGCAATAGCTTTATTCATCTTTTATTGTGGA
AATCTTCTTCTACATTTATTTGGTATCACTTATGGAAAAATTAAATTGCACAAGAAACCA
TGTCTACTGCCACGGGAATCGCCACTTCCATCAGTCTCAATTCTAAAGCCTTTAATGGGC
ATCGATCCGAATTTGTCTGCAAATTTAGAAACATTTTTTACTATGCAATATCCATCCTAT
GAGCTGTTATTTTGTATAGAAGATAAGGAAGATCCTGCTGTGGCAGTAGTTAATAATTTA
ATAGATAAATATCCAAAAGTAGATGCTTATCTGTTTCTTGGTGGATCAAAAGTCGGTGTT
AATCCGAAAATCAACAATTTGAATGCAGGTTACGAAAAGGCAAAATATGAACTTATTATG
ATTTCCGATAGTGGTATTCGTATGAAAGAAGACACCTTGCTTGACATGGTGCAACATATG
AAAGAAGGAATAGCTCTCGTTCATCAAATGCCTTTCACATGTGATCGTGAAGGATTTGCT
GCAACTTTTGAGAAAATATACTTTGGAACATACCAATCAAGAATGTATCTCACGGCTGAT
CTCCTTGGTGCCATTTGTCATACTGGAATGTCATCACTCATTCGTCGCGATGTTTTAGAT
GAACTTGGTGGTTTAAGAGCTTTTGGCTGTTATCTAGCAGAAGATTTCTTCCTTGCAAAA
GCTATAGCTGAAGCAGGATACAAGTCAGCAATCAGTAGTCAACCTGCGTGGCAAAACTCA
GGCATTTGCGATATTACCAACTTTCAATTACGGCTCACGCGATGGGCAAAATTACGGGTT
GCAATGGTGCCGCATTTAATAATTCTTGAACCACTTAGTGAATGTATGGTACTAGGATGT
TTAGCTTCATGGGCAGTAAATGTGTTGTTTCAGTGGGACGCCCTTCCATTTTATTTAGTA
CATATCTTAGTATGGTTCCTCTCCGATTGGATCATGCTATCCGTAGTACAGAATGGACCA
TTGCCGTTTAACAAATTTAATTTTGTTGTCGGATGGCTGTTTCGAGAGTTTACTGGACCA
TATCTATTTCTTTGTGCATTATTTAATCCAGCAATAAGATGGCGTGAAAGGACATTCAAA
CTTCATTGGGGAGGGATCGCTTATGAGACGAAATCGAGAATAAAATCATAG
>g8077.t1 Gene=g8077 Length=396
MSFSIYIYGFAIALFIFYCGNLLLHLFGITYGKIKLHKKPCLLPRESPLPSVSILKPLMG
IDPNLSANLETFFTMQYPSYELLFCIEDKEDPAVAVVNNLIDKYPKVDAYLFLGGSKVGV
NPKINNLNAGYEKAKYELIMISDSGIRMKEDTLLDMVQHMKEGIALVHQMPFTCDREGFA
ATFEKIYFGTYQSRMYLTADLLGAICHTGMSSLIRRDVLDELGGLRAFGCYLAEDFFLAK
AIAEAGYKSAISSQPAWQNSGICDITNFQLRLTRWAKLRVAMVPHLIILEPLSECMVLGC
LASWAVNVLFQWDALPFYLVHILVWFLSDWIMLSVVQNGPLPFNKFNFVVGWLFREFTGP
YLFLCALFNPAIRWRERTFKLHWGGIAYETKSRIKS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g8077.t1 | CDD | cd02520 | Glucosylceramide_synthase | 50 | 279 | 7.60931E-91 |
| 8 | g8077.t1 | Gene3D | G3DSA:3.90.550.10 | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A | 42 | 258 | 6.7E-16 |
| 2 | g8077.t1 | PANTHER | PTHR12726 | CERAMIDE GLUCOSYLTRANSFERASE | 11 | 387 | 2.8E-124 |
| 3 | g8077.t1 | PANTHER | PTHR12726:SF1 | CERAMIDE GLUCOSYLTRANSFERASE 1-RELATED | 11 | 387 | 2.8E-124 |
| 1 | g8077.t1 | Pfam | PF13506 | Glycosyl transferase family 21 | 105 | 277 | 7.3E-52 |
| 12 | g8077.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 15 | g8077.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 29 | - |
| 9 | g8077.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 30 | 279 | - |
| 13 | g8077.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 280 | 306 | - |
| 11 | g8077.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 307 | 317 | - |
| 14 | g8077.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 318 | 336 | - |
| 10 | g8077.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 337 | 396 | - |
| 7 | g8077.t1 | SUPERFAMILY | SSF53448 | Nucleotide-diphospho-sugar transferases | 39 | 275 | 1.78E-23 |
| 6 | g8077.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 27 | - |
| 5 | g8077.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 314 | 333 | - |
| 4 | g8077.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 348 | 370 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016757 | glycosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00720 (ceramide glucosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
