General information for g8077.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Ceramide glucosyltransferase.
Parent gene
- The parent gene g8077 is located on chr_2: 28488535..28488889 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8077 | g8077.t4 | TSS | g8077.t4 | 28483392 | 28483392 |
| chr_2 | g8077 | g8077.t4 | isoform | g8077.t4 | 28484172 | 28488311 |
| chr_2 | g8077 | g8077.t4 | exon | g8077.t4.exon1 | 28484172 | 28484691 |
| chr_2 | g8077 | g8077.t4 | exon | g8077.t4.exon2 | 28485930 | 28486156 |
| chr_2 | g8077 | g8077.t4 | cds | g8077.t4.CDS1 | 28486146 | 28486156 |
| chr_2 | g8077 | g8077.t4 | exon | g8077.t4.exon3 | 28487534 | 28488311 |
| chr_2 | g8077 | g8077.t4 | cds | g8077.t4.CDS2 | 28487534 | 28488311 |
| chr_2 | g8077 | g8077.t4 | TTS | g8077.t4 | 28488950 | 28488950 |
Sequences
>g8077.t4 Gene=g8077 Length=1525
TACTATATTCTGTATAGGGTACAAATAAAGAGAGGAGAATTTCATGATTAAAAAGGAAAT
ATCGCGATTCGAATCGTTTGAAAGGATTGCGTGTTTAATATTTCGGTTTGCCTTGCTACT
GTAATAACCGTCATCTAAACCAATCCTTCTTTTTTCTCGCTCTTCATATTACGGGGCATA
AAAATGAGAAAGTAACCAAACAGTAGGCGAAAAATAAGTTACTAAAAAATTCGACGAGAA
CATTTGTTGGCGCTTATTTGACGGAATTTTCTCTTCTCTTCCACTTGCTACGAGAGTGTC
TCAATAACTTCTATTTTTTTTTACAATTAATATCAAAATTTACATCATTCTATATCATAA
ATAATTATATCAAATGAAGTGATTCTTTATAAAATAATTTAGTATAAAACATATTTTGCG
CTTGATGAGTCATTGTTACGAAGAATTTCTATTACGATTATTTCGTCATTAGTGATCAGT
CATTCAACAAAATTATATCAACTCACAAGTATACGAAAATAAAGATTATGATCAATGCTT
TTCAGAAATAGTAATTAATAGAACTGAAATGAAAGGTTATTGAACATTAAAGTTTCTTTC
AAGTTAAAAGCATTTAATATTCTTAGGAAGTTGCTTGACAGCCATGAAAAAGGAAATTAC
CTCCTTACTAATTATGGCGTGTTTTAAATTATTTAGGTTTTCTTTTTTATGCCAAAGCGA
CATAACATAACTGAAAATGAAAAAAAGAAAAATTAAATTGCACAAGAAACCATGTCTACT
GCCACGGGAATCGCCACTTCCATCAGTCTCAATTCTAAAGCCTTTAATGGGCATCGATCC
GAATTTGTCTGCAAATTTAGAAACATTTTTTACTATGCAATATCCATCCTATGAGCTGTT
ATTTTGTATAGAAGATAAGGAAGATCCTGCTGTGGCAGTAGTTAATAATTTAATAGATAA
ATATCCAAAAGTAGATGCTTATCTGTTTCTTGGTGGATCAAAAGTCGGTGTTAATCCGAA
AATCAACAATTTGAATGCAGGTTACGAAAAGGCAAAATATGAACTTATTATGATTTCCGA
TAGTGGTATTCGTATGAAAGAAGACACCTTGCTTGACATGGTGCAACATATGAAAGAAGG
AATAGCTCTCGTTCATCAAATGCCTTTCACATGTGATCGTGAAGGATTTGCTGCAACTTT
TGAGAAAATATACTTTGGAACATACCAATCAAGAATGTATCTCACGGCTGATCTCCTTGG
TGCCATTTGTCATACTGGAATGTCATCACTCATTCGTCGCGATGTTTTAGATGAACTTGG
TGGTTTAAGAGCTTTTGGCTGTTATCTAGCAGAAGATTTCTTCCTTGCAAAAGCTATAGC
TGAAGCAGGATACAAGTCAGCAATCAGTAGTCAACCTGCGTGGCAAAACTCAGGCATTTG
CGATATTACCAACTTTCAATTACGGCTCACGCGATGGGCAAAATTACGGGTTGCAATGGT
GCCGCATTTAATAATTCTTGAACCA
>g8077.t4 Gene=g8077 Length=263
MKKRKIKLHKKPCLLPRESPLPSVSILKPLMGIDPNLSANLETFFTMQYPSYELLFCIED
KEDPAVAVVNNLIDKYPKVDAYLFLGGSKVGVNPKINNLNAGYEKAKYELIMISDSGIRM
KEDTLLDMVQHMKEGIALVHQMPFTCDREGFAATFEKIYFGTYQSRMYLTADLLGAICHT
GMSSLIRRDVLDELGGLRAFGCYLAEDFFLAKAIAEAGYKSAISSQPAWQNSGICDITNF
QLRLTRWAKLRVAMVPHLIILEP
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g8077.t4 | CDD | cd02520 | Glucosylceramide_synthase | 22 | 251 | 2.51889E-93 |
| 6 | g8077.t4 | Gene3D | G3DSA:3.90.550.10 | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A | 14 | 230 | 2.0E-16 |
| 2 | g8077.t4 | PANTHER | PTHR12726 | CERAMIDE GLUCOSYLTRANSFERASE | 8 | 263 | 1.2E-88 |
| 3 | g8077.t4 | PANTHER | PTHR12726:SF1 | CERAMIDE GLUCOSYLTRANSFERASE 1-RELATED | 8 | 263 | 1.2E-88 |
| 1 | g8077.t4 | Pfam | PF13506 | Glycosyl transferase family 21 | 77 | 249 | 2.5E-52 |
| 4 | g8077.t4 | SUPERFAMILY | SSF53448 | Nucleotide-diphospho-sugar transferases | 11 | 229 | 7.97E-24 |
| 5 | g8077.t4 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 10 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016757 | glycosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00720 (ceramide glucosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
