General information for g8096.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1.
Parent gene
- The parent gene g8096 is located on chr_2: 28563370..28572173 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8096 | g8096.t5 | isoform | g8096.t5 | 28559532 | 28560859 |
| chr_2 | g8096 | g8096.t5 | exon | g8096.t5.exon1 | 28559532 | 28560110 |
| chr_2 | g8096 | g8096.t5 | cds | g8096.t5.CDS1 | 28559542 | 28560110 |
| chr_2 | g8096 | g8096.t5 | exon | g8096.t5.exon2 | 28560178 | 28560859 |
| chr_2 | g8096 | g8096.t5 | cds | g8096.t5.CDS2 | 28560178 | 28560859 |
| chr_2 | g8096 | g8096.t5 | TTS | g8096.t5 | 28561796 | 28561796 |
| chr_2 | g8096 | g8096.t5 | TSS | g8096.t5 | NA | NA |
Sequences
>g8096.t5 Gene=g8096 Length=1261
CGAAGAAAAAATGGATATGATTAAAAATAAAAAGAGAATTCTTTCTCAAGGAACTGATTT
GTTCAATCAACGACCAGAAAAGGGTATACAATTTTTGCAAGAAAATGGTTTAATCAATGC
AACACTTGATCCACTTGAAATTGCACATTTTCTCAGAGAGTCACCTGGACTTGATAAGAA
AATGATTGGAGAATATATCAGCAAAAAAAAGAATGTAGAAAATCGCATTTTGGAAGTTTT
TGTTAAATCTTTTGATTTTACTTCAATGCGTATAGACAAAGCACTACGATTGTATCTTGA
AACATTTCGATTACCAGGTGAAGCTCCATTAATATTTTTAGTAATGGAGCATTTTGCAGA
TCATTGGCATAAATGTAATAATGAACCGTTTGCAAACACCGATGCAGCTTTCAGACTTGC
TTACGCCATTATTATGCTTAATATGGATCAGCATAATCACAATGCTAAAAAGCTCAACAT
TCCAATGACAGTTGAAGATTTTACAAAAAATTTAAGAGGACTTAATGGAAATTCAGATTT
TGATCAAGAAATGCTGATGGAAATTTTCAATTCTATTAAAAATGAAGAAATCATAATGCC
TGCTGAACAAACTGGTATTGTAAAAGAAAATTATTTATGGAAAGTACTATTGCGAAGAGG
TGAAGGTAAAGATGCTGAATTTACTCATGTATTGGATTCTAGTTATGACAAGCAGCTATT
TCAAGTAACATGGGGATCGACTCTTACTGCATTTGGTTCACTATTCGATAAAGTCTCTGA
AATTGCAGTTATTAAAAAAATAATTGAAGGATTTATGCATTCTGCTGCGATTTGTTCACA
TTATAGTCTTCATCAAGAGTTTGATGCCATCATATTAACTCTATGCAAATTCACTACACT
TTACAATACAAACGAAACAAATGAACTTACTATAAGTGTTCAATTTGGTAGTAATTCTAA
AGCAAGGACAGCATTGAAATCAATTTTTAAATTTCTTCACGATTATGGTGATTCAATGCG
AGAAAGTTGGAAACATGTTATAGACCTTTTAGTTCAGATGTACAAATTAAAATTATTACC
AAAATCATTTGTAGAAATTGAAGATTTCTGCGAACCCAGTGGTAAACTTTTGTTACAATA
CGAACCTATTCCTATTCCAAAATCTGAAGCTAGTTTGTTAAGCAGTCTTTATCTATATCT
AAGCTCAGAAAGTCAACGGCAACCTACACATGAAGAACATGAAATATTAAAAATTGCTAA
A
>g8096.t5 Gene=g8096 Length=417
MDMIKNKKRILSQGTDLFNQRPEKGIQFLQENGLINATLDPLEIAHFLRESPGLDKKMIG
EYISKKKNVENRILEVFVKSFDFTSMRIDKALRLYLETFRLPGEAPLIFLVMEHFADHWH
KCNNEPFANTDAAFRLAYAIIMLNMDQHNHNAKKLNIPMTVEDFTKNLRGLNGNSDFDQE
MLMEIFNSIKNEEIIMPAEQTGIVKENYLWKVLLRRGEGKDAEFTHVLDSSYDKQLFQVT
WGSTLTAFGSLFDKVSEIAVIKKIIEGFMHSAAICSHYSLHQEFDAIILTLCKFTTLYNT
NETNELTISVQFGSNSKARTALKSIFKFLHDYGDSMRESWKHVIDLLVQMYKLKLLPKSF
VEIEDFCEPSGKLLLQYEPIPIPKSEASLLSSLYLYLSSESQRQPTHEEHEILKIAK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g8096.t5 | CDD | cd00171 | Sec7 | 7 | 194 | 0.000 |
| 7 | g8096.t5 | Gene3D | G3DSA:1.10.220.20 | - | 1 | 83 | 0.000 |
| 6 | g8096.t5 | Gene3D | G3DSA:1.10.1000.11 | - | 84 | 209 | 0.000 |
| 2 | g8096.t5 | PANTHER | PTHR10663:SF316 | GOLGI-SPECIFIC BREFELDIN A-RESISTANCE GUANINE NUCLEOTIDE EXCHANGE FACTOR 1 | 4 | 412 | 0.000 |
| 3 | g8096.t5 | PANTHER | PTHR10663 | GUANYL-NUCLEOTIDE EXCHANGE FACTOR | 4 | 412 | 0.000 |
| 1 | g8096.t5 | Pfam | PF01369 | Sec7 domain | 7 | 194 | 0.000 |
| 8 | g8096.t5 | ProSiteProfiles | PS50190 | SEC7 domain profile. | 5 | 192 | 38.057 |
| 5 | g8096.t5 | SMART | SM00222 | sec7_5 | 4 | 194 | 0.000 |
| 4 | g8096.t5 | SUPERFAMILY | SSF48425 | Sec7 domain | 4 | 202 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0032012 | regulation of ARF protein signal transduction | BP |
| GO:0005085 | guanyl-nucleotide exchange factor activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed