General information for g8105.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable cytochrome P450 12a5, mitochondrial.
Parent gene
- The parent gene g8105 is located on chr_2: 28582518..28583171 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8105 | g8105.t4 | TTS | g8105.t4 | 28581104 | 28581104 |
| chr_2 | g8105 | g8105.t4 | isoform | g8105.t4 | 28581967 | 28583171 |
| chr_2 | g8105 | g8105.t4 | exon | g8105.t4.exon1 | 28581967 | 28582073 |
| chr_2 | g8105 | g8105.t4 | cds | g8105.t4.CDS1 | 28581993 | 28582073 |
| chr_2 | g8105 | g8105.t4 | exon | g8105.t4.exon2 | 28582146 | 28582299 |
| chr_2 | g8105 | g8105.t4 | cds | g8105.t4.CDS2 | 28582146 | 28582299 |
| chr_2 | g8105 | g8105.t4 | exon | g8105.t4.exon3 | 28582709 | 28582825 |
| chr_2 | g8105 | g8105.t4 | cds | g8105.t4.CDS3 | 28582709 | 28582825 |
| chr_2 | g8105 | g8105.t4 | exon | g8105.t4.exon4 | 28582897 | 28583171 |
| chr_2 | g8105 | g8105.t4 | cds | g8105.t4.CDS4 | 28582897 | 28583171 |
| chr_2 | g8105 | g8105.t4 | TSS | g8105.t4 | 28583368 | 28583368 |
Sequences
>g8105.t4 Gene=g8105 Length=653
ATGATTAGACATTTTGCAATACCTGGGGGAAAGTTTTACAATAAACCACTTAACGAATTA
CTTCAATTGTTAAGAAAGGAATATGGCGACATTTTTTACTTTCCGGGAATACTTGGTCAA
AAGCCGCTTATTATGACATTCAAACCAGAAGATGCAGAGAAAGTTTTTAGAGTTGAAGGT
CGCTGGCCGTACAGACGTCCTTTAGAAACCTTTACACATTACAGACGCAATTATAGACAA
GATATTTTTGAAAGTAGTGGAGGCTTAACAGTTGAACATGGTGAAAAATGGCATGATTTT
CGAACAAAAGTAAACCAAATCATGATGCAACCAAAAAACACGAAGATGTACATCCCAAAA
ATTGATGAAGTAACAATGGATTTCATTGATAAAATGCGTGCAATAAGGAACGAGAATAAT
GAAATGCCTGCTGATTTTTTTGAAACATTAAATGAATGGTCGCTTGAAAGTATAGCGCTT
ATAGCTCTAGATACGAGATTAGGGCTATTAAAAGGAAATAATCCTGAAGCACAAAAGCTC
TATGAATTTTTACAGCCTCTTATGAATATGACATACAGCCCAGTATTTGGAGATATTATC
AAACACCAGGATTTAAGAAAGCAATGAAGTCTTTTGACGCTGTAACAGATACG
>g8105.t4 Gene=g8105 Length=208
MIRHFAIPGGKFYNKPLNELLQLLRKEYGDIFYFPGILGQKPLIMTFKPEDAEKVFRVEG
RWPYRRPLETFTHYRRNYRQDIFESSGGLTVEHGEKWHDFRTKVNQIMMQPKNTKMYIPK
IDEVTMDFIDKMRAIRNENNEMPADFFETLNEWSLESIALIALDTRLGLLKGNNPEAQKL
YEFLQPLMNMTYSPVFGDIIKHQDLRKQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g8105.t4 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 5 | 207 | 0 |
| 2 | g8105.t4 | PANTHER | PTHR24291:SF48 | - | 20 | 192 | 0 |
| 3 | g8105.t4 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 20 | 192 | 0 |
| 1 | g8105.t4 | Pfam | PF00067 | Cytochrome P450 | 15 | 189 | 0 |
| 4 | g8105.t4 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 15 | 197 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed