Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative NEDD8-conjugating enzyme UBE2F.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g8110 g8110.t1 TTS g8110.t1 28624976 28624976
chr_2 g8110 g8110.t1 isoform g8110.t1 28625010 28625672
chr_2 g8110 g8110.t1 exon g8110.t1.exon1 28625010 28625236
chr_2 g8110 g8110.t1 cds g8110.t1.CDS1 28625010 28625236
chr_2 g8110 g8110.t1 exon g8110.t1.exon2 28625302 28625537
chr_2 g8110 g8110.t1 cds g8110.t1.CDS2 28625302 28625537
chr_2 g8110 g8110.t1 exon g8110.t1.exon3 28625599 28625672
chr_2 g8110 g8110.t1 cds g8110.t1.CDS3 28625599 28625672
chr_2 g8110 g8110.t1 TSS g8110.t1 28625761 28625761

Sequences

>g8110.t1 Gene=g8110 Length=537
ATGATAACATTAACACGAAATATAAAAAAGTCGAATGATGTGAATGGTCAAAACAATAAA
CGCATCTCTATAAGAGATAAATTATTAGTAAAAGAGATTCAAGAAATGGAGTTATGCAAA
CCATCATCAGTGACATGTGAGTTTCCTGATTCAAATAATTTGAGTGAATTTTACATCATA
GTCACACCAGGAGATGAATCTTTATGGAGAAATGGAAGTTTCAAGTTTCTTGTAACTGTA
AATGAAGATTACAATATGATTCCACCAACAGTCAAGTGTTTAACAAAAGTTTTACATCCT
AATATTTCTTCTAATGGAGATGTATGCTTAAGTTTGTTGAGACTTAATTCTATAGATGGA
ACTAGCTGGTTACCAACTCGCCGCATGAAGGATGTCATGTTTGCACTGAATAGTCTTTTT
AGTGATTTGGTAGATTTTTCAGATCCATTAAACGTTGAATTAGCTGAAGAATACTCAAAA
GATAGTGAAAAGGCGAAAATGAAAATAAGAGAATATGTATTGAAACATGCTAGATAA

>g8110.t1 Gene=g8110 Length=178
MITLTRNIKKSNDVNGQNNKRISIRDKLLVKEIQEMELCKPSSVTCEFPDSNNLSEFYII
VTPGDESLWRNGSFKFLVTVNEDYNMIPPTVKCLTKVLHPNISSNGDVCLSLLRLNSIDG
TSWLPTRRMKDVMFALNSLFSDLVDFSDPLNVELAEEYSKDSEKAKMKIREYVLKHAR

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g8110.t1 CDD cd00195 UBCc 27 173 6.33826E-37
5 g8110.t1 Gene3D G3DSA:3.10.110.10 Ubiquitin Conjugating Enzyme 12 178 1.4E-47
2 g8110.t1 PANTHER PTHR24068:SF129 NEDD8-CONJUGATING ENZYME UBE2F-RELATED 15 173 8.3E-54
3 g8110.t1 PANTHER PTHR24068 UBIQUITIN-CONJUGATING ENZYME E2 15 173 8.3E-54
1 g8110.t1 Pfam PF00179 Ubiquitin-conjugating enzyme 29 172 3.1E-29
7 g8110.t1 ProSitePatterns PS00183 Ubiquitin-conjugating enzymes active site. 98 113 -
9 g8110.t1 ProSiteProfiles PS50127 Ubiquitin-conjugating enzymes family profile. 27 167 24.153
8 g8110.t1 SMART SM00212 ubc_7 27 178 3.0E-37
4 g8110.t1 SUPERFAMILY SSF54495 UBC-like 7 177 2.12E-39

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values