Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative NEDD8-conjugating enzyme UBE2F.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g8110 g8110.t6 TTS g8110.t6 28624976 28624976
chr_2 g8110 g8110.t6 isoform g8110.t6 28625010 28625539
chr_2 g8110 g8110.t6 exon g8110.t6.exon1 28625010 28625236
chr_2 g8110 g8110.t6 cds g8110.t6.CDS1 28625010 28625236
chr_2 g8110 g8110.t6 exon g8110.t6.exon2 28625302 28625539
chr_2 g8110 g8110.t6 cds g8110.t6.CDS2 28625302 28625506
chr_2 g8110 g8110.t6 TSS g8110.t6 28625761 28625761

Sequences

>g8110.t6 Gene=g8110 Length=465
AGAGATAAATTATTAGTAAAAGAGATTCAAGAAATGGAGTTATGCAAACCATCATCAGTG
ACATGTGAGTTTCCTGATTCAAATAATTTGAGTGAATTTTACATCATAGTCACACCAGGA
GATGAATCTTTATGGAGAAATGGAAGTTTCAAGTTTCTTGTAACTGTAAATGAAGATTAC
AATATGATTCCACCAACAGTCAAGTGTTTAACAAAAGTTTTACATCCTAATATTTCTTCT
AATGGAGATGTATGCTTAAGTTTGTTGAGACTTAATTCTATAGATGGAACTAGCTGGTTA
CCAACTCGCCGCATGAAGGATGTCATGTTTGCACTGAATAGTCTTTTTAGTGATTTGGTA
GATTTTTCAGATCCATTAAACGTTGAATTAGCTGAAGAATACTCAAAAGATAGTGAAAAG
GCGAAAATGAAAATAAGAGAATATGTATTGAAACATGCTAGATAA

>g8110.t6 Gene=g8110 Length=143
MELCKPSSVTCEFPDSNNLSEFYIIVTPGDESLWRNGSFKFLVTVNEDYNMIPPTVKCLT
KVLHPNISSNGDVCLSLLRLNSIDGTSWLPTRRMKDVMFALNSLFSDLVDFSDPLNVELA
EEYSKDSEKAKMKIREYVLKHAR

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g8110.t6 CDD cd00195 UBCc 1 138 4.42749E-33
5 g8110.t6 Gene3D G3DSA:3.10.110.10 Ubiquitin Conjugating Enzyme 1 143 2.5E-42
2 g8110.t6 PANTHER PTHR24068:SF129 NEDD8-CONJUGATING ENZYME UBE2F-RELATED 6 138 3.6E-45
3 g8110.t6 PANTHER PTHR24068 UBIQUITIN-CONJUGATING ENZYME E2 6 138 3.6E-45
1 g8110.t6 Pfam PF00179 Ubiquitin-conjugating enzyme 5 137 8.4E-28
7 g8110.t6 ProSitePatterns PS00183 Ubiquitin-conjugating enzymes active site. 63 78 -
9 g8110.t6 ProSiteProfiles PS50127 Ubiquitin-conjugating enzymes family profile. 1 132 22.154
8 g8110.t6 SMART SM00212 ubc_7 1 143 1.2E-29
4 g8110.t6 SUPERFAMILY SSF54495 UBC-like 5 142 5.37E-35

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

There are no GO annotations for this transcript.

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values