General information for g8126.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as cAMP-dependent protein kinase catalytic subunit beta.
Parent gene
- The parent gene g8126 is located on chr_2: 28686011..28687201 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8126 | g8126.t1 | isoform | g8126.t1 | 28686011 | 28687201 |
| chr_2 | g8126 | g8126.t1 | exon | g8126.t1.exon1 | 28686011 | 28686202 |
| chr_2 | g8126 | g8126.t1 | cds | g8126.t1.CDS1 | 28686011 | 28686202 |
| chr_2 | g8126 | g8126.t1 | exon | g8126.t1.exon2 | 28686260 | 28686489 |
| chr_2 | g8126 | g8126.t1 | cds | g8126.t1.CDS2 | 28686260 | 28686489 |
| chr_2 | g8126 | g8126.t1 | exon | g8126.t1.exon3 | 28686544 | 28687201 |
| chr_2 | g8126 | g8126.t1 | cds | g8126.t1.CDS3 | 28686544 | 28687201 |
| chr_2 | g8126 | g8126.t1 | TTS | g8126.t1 | 28687293 | 28687293 |
| chr_2 | g8126 | g8126.t1 | TSS | g8126.t1 | NA | NA |
Sequences
>g8126.t1 Gene=g8126 Length=1080
ATGACAGAAAACAATTCAAAATCTATAACAATTATCAAACCTAAAACATCTGATGATCTG
AAGAAAGTTATGACAAATTTAAAAACAGAATTTGATCTCAAGTATAACAACGATGAGCCA
TCTTTGTCGAGTGATCGTCTTAATGAATTTCAATTTTTGACAATTTTGGGACAAGGAGCT
TTTGGTGTTGTTAAACTTATAAAACATAATACCACTGAAAAGTTCTATGCTGTTAAAATT
ATTAGCAAAGAACGTATTGTTAAAAGCAAGCAAATTTTTCATTGTATCAATGAGAAGAAA
ATTCTTCGATCACTCAATTATCCTTTCGTTGTCTACTTAGAGTTTTCATTGAAAGATAAT
AGTTATGTTTACTTCTGTATGCCTTTCGTCAATGGTGGAGAAATGTTTACGCATTTGAGA
AAAGCGAAAAAATTCGAGGAAGAACTTTCGAAATTTTATGCGGCACAAGTTTTTATGGCG
CTTGATTATATTCATAATTTGGGAATGATTTATAGAGATTTAAAGCCCGAAAATATTATG
ATTGATTATAGAGGATTTATAAAAGTGACAGATTTTGGCTTCTGCAAGCCAATCAAAGAT
CGAACATACACATTATGTGGTACGCCAGAATATTTGGCGCCTGAAGTTATTAAGAACAAA
GGATATGGTCAAAGTGCTGATTGGTGGTCATTTGGAATTTTGATTTTTGAAATGTCATCT
GGCTATTCACCATTTGCTGTTGGTCATCCCGATCAAATGCAATTACTTGAGCGAATATGT
ATTGGAAAATATAGAATGTCAACTTCATTTGGTAAAGAATTGAAATCATTGATTTCAAAT
ATTTTGCAAATTGATCTAACGCGTCGTTTTGGTAATTTAAAAAATGGAAGTGAAGACATA
AAAACACATCCGTGGTTTAATGATACTGACTGGGTGGCATTGTATAATCAAGAGATCAAA
CCGCCTTTCATACCAAAATTAACTGGACCTGGTGACTACTCGCAATTTGACGAATACGAG
GATGTAAAACTCAAAATTGCTCATACATGTGTATTTGAAAAGGAATTTGCCGACTTTTAA
>g8126.t1 Gene=g8126 Length=359
MTENNSKSITIIKPKTSDDLKKVMTNLKTEFDLKYNNDEPSLSSDRLNEFQFLTILGQGA
FGVVKLIKHNTTEKFYAVKIISKERIVKSKQIFHCINEKKILRSLNYPFVVYLEFSLKDN
SYVYFCMPFVNGGEMFTHLRKAKKFEEELSKFYAAQVFMALDYIHNLGMIYRDLKPENIM
IDYRGFIKVTDFGFCKPIKDRTYTLCGTPEYLAPEVIKNKGYGQSADWWSFGILIFEMSS
GYSPFAVGHPDQMQLLERICIGKYRMSTSFGKELKSLISNILQIDLTRRFGNLKNGSEDI
KTHPWFNDTDWVALYNQEIKPPFIPKLTGPGDYSQFDEYEDVKLKIAHTCVFEKEFADF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g8126.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 48 | 338 | 4.0E-112 |
| 10 | g8126.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 134 | 326 | 4.0E-112 |
| 2 | g8126.t1 | PANTHER | PTHR24353:SF117 | UT01108P-RELATED | 34 | 359 | 1.9E-139 |
| 3 | g8126.t1 | PANTHER | PTHR24353 | CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE | 34 | 359 | 1.9E-139 |
| 1 | g8126.t1 | Pfam | PF00069 | Protein kinase domain | 51 | 306 | 4.4E-67 |
| 8 | g8126.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 56 | 88 | - |
| 7 | g8126.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 169 | 181 | - |
| 12 | g8126.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 50 | 306 | 45.827 |
| 11 | g8126.t1 | ProSiteProfiles | PS51285 | AGC-kinase C-terminal domain profile. | 307 | 359 | 12.749 |
| 6 | g8126.t1 | SMART | SM00220 | serkin_6 | 50 | 306 | 2.2E-90 |
| 5 | g8126.t1 | SMART | SM00133 | pkinase_C_6 | 307 | 359 | 1.8E-9 |
| 4 | g8126.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 41 | 341 | 1.62E-86 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
