General information for g8132.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4d2.
Parent gene
- The parent gene g8132 is located on chr_2: 28722381..28724549 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8132 | g8132.t3 | TSS | g8132.t3 | 28720199 | 28720199 |
| chr_2 | g8132 | g8132.t3 | isoform | g8132.t3 | 28720256 | 28721978 |
| chr_2 | g8132 | g8132.t3 | exon | g8132.t3.exon1 | 28720256 | 28720790 |
| chr_2 | g8132 | g8132.t3 | cds | g8132.t3.CDS1 | 28720256 | 28720790 |
| chr_2 | g8132 | g8132.t3 | exon | g8132.t3.exon2 | 28720849 | 28720918 |
| chr_2 | g8132 | g8132.t3 | cds | g8132.t3.CDS2 | 28720849 | 28720918 |
| chr_2 | g8132 | g8132.t3 | exon | g8132.t3.exon3 | 28720997 | 28721300 |
| chr_2 | g8132 | g8132.t3 | cds | g8132.t3.CDS3 | 28720997 | 28721300 |
| chr_2 | g8132 | g8132.t3 | exon | g8132.t3.exon4 | 28721694 | 28721805 |
| chr_2 | g8132 | g8132.t3 | cds | g8132.t3.CDS4 | 28721694 | 28721780 |
| chr_2 | g8132 | g8132.t3 | exon | g8132.t3.exon5 | 28721872 | 28721978 |
| chr_2 | g8132 | g8132.t3 | TTS | g8132.t3 | 28722495 | 28722495 |
Sequences
>g8132.t3 Gene=g8132 Length=1128
ATGTTGATTGTAATTTTAACACTTACAATTGTACTTTTTTGGCTTTTAAGGGATGAATTT
AGAAAATTTTATTTAGCATTTTTTATTCCTGGTCCTCCAGGATTACCATTGGCAGGAAAT
GGATTAGATTTAATTAATAAATCACCACAAGAGTTGATTCAAATTATTTCGCGCTATTTA
AAAAATCATGGAAATTTTATTAGAATATGGCTTGGAAATCAACTCTTAATTGCAACTACA
GATCCAAAAGCCGTGCAAGCTATAATGACTGATAGCAATTTATTAACCAAATCGAGAGAA
TACGAATATATGGAAGCATGGCTTGGTACCGGTTTACTCACGTCAACAAATCAAAAGTGG
TTAAGTCGTCGTAAAATTCTTACACCTGCATTTCATTTTAAAATTCTAGATGAATTCATT
GAAGTTTTTGACAAGCAAGGCACAATTTTGATTGAAAAGCTAAAAAAATTTGATGGAAAA
GAATCATTTAATATATTTCCACTTGTTGCATTATGTGCTTTGGATGTCATTTGTGAAACT
GCTATGGGAATTTCACTCAATTCACAATCAGATTCAGAGTCGCAATATGTTAAAAATGTT
AATGAACTTGCCGATATTATACATCGTCGCACATATGATTTTATTGAACGATATGAATTT
TTGTTCAAATTCACCAAAACTTATAAACGACAGCAAAAGTTAGTTGAAGCTCTTCATCAG
TTCACGGAGTCAGTCATCAAATCACGTCGTAAAGAAATGAAGAGTAAACAAAATACTGCA
CAGGAAGACGAAGATTTTGGCATCAAGAAAAAGAAAGCTCTGCTTGATCTGTTACTAGAA
ACAACAGTTGAAGGAAAGCTATTGAGTAATGAAGACATTCGTGAGGAAATCGACACTTTT
ATGTTTGAGCAAGTGCACTCGCTTATACACTTTATAACATTGCGAGATATGAACACATTC
AGAAAAAATGTTTTGAAGAAATTGACAGTGTGCTAGGAAATGATGGGAAAATTACTATGA
AGTCACTTAATGAGTTGAAATATTTAGAACTTGTCTGCAAAGAATCATTGAGATTATATC
CATCTGTTCCAATTTTTGGTCGAAGAACAGTTGAAGAAATGATGATAA
>g8132.t3 Gene=g8132 Length=331
MLIVILTLTIVLFWLLRDEFRKFYLAFFIPGPPGLPLAGNGLDLINKSPQELIQIISRYL
KNHGNFIRIWLGNQLLIATTDPKAVQAIMTDSNLLTKSREYEYMEAWLGTGLLTSTNQKW
LSRRKILTPAFHFKILDEFIEVFDKQGTILIEKLKKFDGKESFNIFPLVALCALDVICET
AMGISLNSQSDSESQYVKNVNELADIIHRRTYDFIERYEFLFKFTKTYKRQQKLVEALHQ
FTESVIKSRRKEMKSKQNTAQEDEDFGIKKKKALLDLLLETTVEGKLLSNEDIREEIDTF
MFEQVHSLIHFITLRDMNTFRKNVLKKLTVC
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g8132.t3 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 17 | 313 | 2.2E-48 |
| 2 | g8132.t3 | PANTHER | PTHR24291:SF143 | CYTOCHROME P450 4D1-RELATED | 5 | 303 | 4.5E-88 |
| 3 | g8132.t3 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 5 | 303 | 4.5E-88 |
| 1 | g8132.t3 | Pfam | PF00067 | Cytochrome P450 | 30 | 303 | 2.8E-43 |
| 7 | g8132.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 8 | g8132.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 1 | - |
| 9 | g8132.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 2 | 13 | - |
| 10 | g8132.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 17 | - |
| 6 | g8132.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 331 | - |
| 4 | g8132.t3 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 29 | 302 | 8.38E-46 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
