Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Probable cytochrome P450 4d14.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g8132 g8132.t9 TSS g8132.t9 28721717 28721717
chr_2 g8132 g8132.t9 isoform g8132.t9 28722047 28722497
chr_2 g8132 g8132.t9 exon g8132.t9.exon1 28722047 28722497
chr_2 g8132 g8132.t9 cds g8132.t9.CDS1 28722067 28722381
chr_2 g8132 g8132.t9 TTS g8132.t9 28722495 28722495

Sequences

>g8132.t9 Gene=g8132 Length=451
CAAAAGATACAACTCTTCTAATGCTGCCTTACTTTATGGCAAGAGATCCAGAAATTTGGC
AAAATCCTGAAAAATTTATTCCTGAAAGACACGCTGTTGAAAAGGATAACGAAGATTCGC
AAGTTTTTACATACATTCCTTTCTCGGGTGGTTACCGAAATTGTATTGGACAAAAATTCG
CTATGTTAGAACTTAAATCGACCGTCTCAAAAGTAGTATTGAATTACAAATTAAGTGTAA
AAGAAAATTTCACTCCTGAAGACGCTCTTGAACTTGTCATAAAAAGTACTAATGGAATAA
TGCTCAAAATTGAAAGTAGAAAATTAAGCAATTAAATAATTACTGTTGTTAAATTGCTTA
AGGTTAAGGGCCATAAACATTTTTTATTTTGTACATGATATTTATTTGTTATTATGCATT
TTAATTTTAAAATAAATTTAATTTACCATTT

>g8132.t9 Gene=g8132 Length=104
MLPYFMARDPEIWQNPEKFIPERHAVEKDNEDSQVFTYIPFSGGYRNCIGQKFAMLELKS
TVSKVVLNYKLSVKENFTPEDALELVIKSTNGIMLKIESRKLSN

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g8132.t9 Gene3D G3DSA:1.10.630.10 Cytochrome p450 1 104 2.1E-27
2 g8132.t9 PANTHER PTHR24291:SF143 CYTOCHROME P450 4D1-RELATED 1 100 2.1E-24
3 g8132.t9 PANTHER PTHR24291 CYTOCHROME P450 FAMILY 4 1 100 2.1E-24
4 g8132.t9 PRINTS PR00463 E-class P450 group I signature 2 26 2.9E-9
6 g8132.t9 PRINTS PR00463 E-class P450 group I signature 38 48 2.9E-9
5 g8132.t9 PRINTS PR00463 E-class P450 group I signature 48 71 2.9E-9
1 g8132.t9 Pfam PF00067 Cytochrome P450 2 96 1.7E-24
8 g8132.t9 ProSitePatterns PS00086 Cytochrome P450 cysteine heme-iron ligand signature. 41 50 -
7 g8132.t9 SUPERFAMILY SSF48264 Cytochrome P450 2 100 2.23E-25

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0020037 heme binding MF
GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen MF
GO:0005506 iron ion binding MF
GO:0055114 NA NA

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values