General information for g8133.t12
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable cytochrome P450 12a5, mitochondrial.
Parent gene
- The parent gene g8133 is located on chr_2: 28723582..28724549 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8133 | g8133.t12 | isoform | g8133.t12 | 28723582 | 28724549 |
| chr_2 | g8133 | g8133.t12 | exon | g8133.t12.exon1 | 28723582 | 28723857 |
| chr_2 | g8133 | g8133.t12 | cds | g8133.t12.CDS1 | 28723584 | 28723857 |
| chr_2 | g8133 | g8133.t12 | exon | g8133.t12.exon2 | 28723924 | 28724036 |
| chr_2 | g8133 | g8133.t12 | cds | g8133.t12.CDS2 | 28723924 | 28724036 |
| chr_2 | g8133 | g8133.t12 | exon | g8133.t12.exon3 | 28724108 | 28724204 |
| chr_2 | g8133 | g8133.t12 | cds | g8133.t12.CDS3 | 28724108 | 28724200 |
| chr_2 | g8133 | g8133.t12 | exon | g8133.t12.exon4 | 28724351 | 28724549 |
| chr_2 | g8133 | g8133.t12 | TSS | g8133.t12 | 28724569 | 28724569 |
| chr_2 | g8133 | g8133.t12 | TTS | g8133.t12 | NA | NA |
Sequences
>g8133.t12 Gene=g8133 Length=685
ATGATTTGCAACATTTTGAAGTTTAATAAATTGAGTAATTTGCAAAAGTCATTTGCTTAT
TTTTCCACACAAGTGGCCAAAAAAGAATTTGTTAATTTTCAAGATGAGTGGGAAAATGCA
AGATCATTTGATGAAATTCCTCAACCATCGAAAATTTCATTAATTCGTGCATTTTTACCT
GGAGGTAATTAATAATCAAATCAATGAACAAAGAATATGGAACAATGGTTAAATTTCCTG
AAGTTCTTGGAAGACGTGGAATGGTTTGCAGTTATAATGTCGAAGATGTAGAAAAGCTGT
TTAGACATGAAGGTCAAACGCCTTATCGTAGAACACTCGAAACATTGCATCATTATAGAA
AAAAATATCGACCTGAAGTTTACGAAGAATATGGAGGTTTGTTTACAGAACAAGGCGAGT
CATGGTATAAGATGCGTACGATTGCTAATCCTGTACTGTTACAACCAAAAACAATTAAGC
AGTACATTCCACAGGTTGATGATGTTTCGAAAGAATTTATAAAAATGATCGTTCAAAAAC
TTGATGCCAACAATGAATGTCCTGAAAATTTCAGTGATTATTTGAATTTGTGGAGTCTAG
AGAGCATTGCTAATATTTCTCTCGATAGGCGTCTTGGTATTGTTAGTGGAAATTATCAAG
ACGAAATGGCAGAAAGACTCATAAA
>g8133.t12 Gene=g8133 Length=160
MNKEYGTMVKFPEVLGRRGMVCSYNVEDVEKLFRHEGQTPYRRTLETLHHYRKKYRPEVY
EEYGGLFTEQGESWYKMRTIANPVLLQPKTIKQYIPQVDDVSKEFIKMIVQKLDANNECP
ENFSDYLNLWSLESIANISLDRRLGIVSGNYQDEMAERLI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g8133.t12 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 1 | 160 | 0 |
| 2 | g8133.t12 | PANTHER | PTHR24291:SF48 | - | 2 | 155 | 0 |
| 3 | g8133.t12 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 2 | 155 | 0 |
| 1 | g8133.t12 | Pfam | PF00067 | Cytochrome P450 | 2 | 150 | 0 |
| 4 | g8133.t12 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 3 | 148 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed