General information for g8133.t6
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable cytochrome P450 12b2, mitochondrial.
Parent gene
- The parent gene g8133 is located on chr_2: 28723582..28724549 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8133 | g8133.t6 | TTS | g8133.t6 | 28722419 | 28722419 |
| chr_2 | g8133 | g8133.t6 | isoform | g8133.t6 | 28722513 | 28724549 |
| chr_2 | g8133 | g8133.t6 | exon | g8133.t6.exon1 | 28722513 | 28722687 |
| chr_2 | g8133 | g8133.t6 | exon | g8133.t6.exon2 | 28722746 | 28722809 |
| chr_2 | g8133 | g8133.t6 | cds | g8133.t6.CDS1 | 28722801 | 28722809 |
| chr_2 | g8133 | g8133.t6 | exon | g8133.t6.exon3 | 28722886 | 28723143 |
| chr_2 | g8133 | g8133.t6 | cds | g8133.t6.CDS2 | 28722886 | 28723143 |
| chr_2 | g8133 | g8133.t6 | exon | g8133.t6.exon4 | 28723197 | 28723353 |
| chr_2 | g8133 | g8133.t6 | cds | g8133.t6.CDS3 | 28723197 | 28723353 |
| chr_2 | g8133 | g8133.t6 | exon | g8133.t6.exon5 | 28723412 | 28723527 |
| chr_2 | g8133 | g8133.t6 | cds | g8133.t6.CDS4 | 28723412 | 28723527 |
| chr_2 | g8133 | g8133.t6 | exon | g8133.t6.exon6 | 28723581 | 28723857 |
| chr_2 | g8133 | g8133.t6 | cds | g8133.t6.CDS5 | 28723581 | 28723857 |
| chr_2 | g8133 | g8133.t6 | exon | g8133.t6.exon7 | 28723924 | 28724036 |
| chr_2 | g8133 | g8133.t6 | cds | g8133.t6.CDS6 | 28723924 | 28724036 |
| chr_2 | g8133 | g8133.t6 | exon | g8133.t6.exon8 | 28724108 | 28724204 |
| chr_2 | g8133 | g8133.t6 | cds | g8133.t6.CDS7 | 28724108 | 28724204 |
| chr_2 | g8133 | g8133.t6 | exon | g8133.t6.exon9 | 28724268 | 28724304 |
| chr_2 | g8133 | g8133.t6 | cds | g8133.t6.CDS8 | 28724268 | 28724304 |
| chr_2 | g8133 | g8133.t6 | exon | g8133.t6.exon10 | 28724366 | 28724549 |
| chr_2 | g8133 | g8133.t6 | cds | g8133.t6.CDS9 | 28724366 | 28724549 |
| chr_2 | g8133 | g8133.t6 | TSS | g8133.t6 | 28724569 | 28724569 |
Sequences
>g8133.t6 Gene=g8133 Length=1478
ATGATTTGCAACATTTTGAAGTTTAATAAATTGAGTAATTTGCAAAAGTCATTTGCTTAT
TTTTCCACACAAGTGGCCAAAAAAGAATTTGTTAATTTTCAAGATGAGTGGGAAAATGCA
AGATCATTTGATGAAATTCCTCAACCATCGAAAATTTCATTAATTCGTGCATTTTTACCT
GGAGGAAAATATTACAAACTATCGTTAATTGATATGCATAGATCAATGAACAAAGAATAT
GGAACAATGGTTAAATTTCCTGAAGTTCTTGGAAGACGTGGAATGGTTTGCAGTTATAAT
GTCGAAGATGTAGAAAAGCTGTTTAGACATGAAGGTCAAACGCCTTATCGTAGAACACTC
GAAACATTGCATCATTATAGAAAAAAATATCGACCTGAAGTTTACGAAGAATATGGAGGT
TTGTTTACAGAACAAGGCGAGTCATGGTATAAGATGCGTACGATTGCTAATCCTGTACTG
TTACAACCAAAAACAATTAAGCAGTACATTCCACAGGTTGATGATGTTTCGAAAGAATTT
ATAAAAATGATCGTTCAAAAACTTGATGCCAACAATGAATGTCCTGAAAATTTCAGTGAT
TATTTGAATTTGTGGAGTCTAGAGAGCATTGCTAATATTTCTCTCGATAGGCGTCTTGGT
ATTGTTAGTGGAAATTATCAAGACGAAATGGCAGAAAGACTCATAAAATCAATTAGACAA
TTCTTTGTTGAAGTCTATGAATTTGAAGTTAATATGTCGGTTTGGAAATATTATGAAACG
AAGGCATTTAAAAATCTGATGAACCTTTATGATGAACTTACCAACATTATATTACATTAT
GTTGAGAAAGCCATTGAAGATATTCAAAAGAAAACTATTCGAAATGACAAAGAAGAAAGT
ATTTTGGAAAAATTGTTAAAGATAAATAAAAATGCTGCCATAGTCATGACTGCTGATGCT
CTTTTAGCTGGTGTTGATACGACAAGCTCAGCAACAATAGGCATTTTATATTGCTTGGCA
AAAAATCAAGATAAACAAAACAAATTACGGGAAGAATTGAAATCAATATTAAGGAATGAA
AATGATGAATTGACTCCAAAAAATATGCAAAACATGCCTTATTTACGAGCATGCATTAAA
GAAGGATTTCGACTTTATCCACCAACAGTAGGAACTGTGAGAAAAGCTGATCAAGATTTA
GTCTTGTCTGGTTATAAAATTCCGAAAGGAACTGAAGTTAAATTTTAGACACAAATTGTC
CTCATGCAAAAGAAGCTTCACCATTTTCTTATCTTCCATTTGGGTTCGGTTCACGAGTAT
GTATCGGAAAAAGATTGGCTAATCTTGAAATGGAAGTTCTAATAACTAGGCTTATTCGTG
ATTATAAAATAGAATGGAATTATCCTGAATTGAAATTCAAAGTTGCTATTGTGAATATAC
CTGATGGTGCATTAAAGTTTAAAATTACTAAAATATAA
>g8133.t6 Gene=g8133 Length=415
MICNILKFNKLSNLQKSFAYFSTQVAKKEFVNFQDEWENARSFDEIPQPSKISLIRAFLP
GGKYYKLSLIDMHRSMNKEYGTMVKFPEVLGRRGMVCSYNVEDVEKLFRHEGQTPYRRTL
ETLHHYRKKYRPEVYEEYGGLFTEQGESWYKMRTIANPVLLQPKTIKQYIPQVDDVSKEF
IKMIVQKLDANNECPENFSDYLNLWSLESIANISLDRRLGIVSGNYQDEMAERLIKSIRQ
FFVEVYEFEVNMSVWKYYETKAFKNLMNLYDELTNIILHYVEKAIEDIQKKTIRNDKEES
ILEKLLKINKNAAIVMTADALLAGVDTTSSATIGILYCLAKNQDKQNKLREELKSILRNE
NDELTPKNMQNMPYLRACIKEGFRLYPPTVGTVRKADQDLVLSGYKIPKGTEVKF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g8133.t6 | Coils | Coil | Coil | 339 | 359 | - |
| 5 | g8133.t6 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 35 | 415 | 1.0E-87 |
| 2 | g8133.t6 | PANTHER | PTHR24291:SF155 | CYTOCHROME P450 301B1 | 38 | 413 | 3.2E-82 |
| 3 | g8133.t6 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 38 | 413 | 3.2E-82 |
| 1 | g8133.t6 | Pfam | PF00067 | Cytochrome P450 | 71 | 414 | 3.1E-52 |
| 4 | g8133.t6 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 47 | 413 | 1.57E-56 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed