General information for g8135.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 2-acylglycerol O-acyltransferase 1.
Parent gene
- The parent gene g8135 is located on chr_2: 28746187..28747699 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8135 | g8135.t1 | TSS | g8135.t1 | 28744788 | 28744788 |
| chr_2 | g8135 | g8135.t1 | isoform | g8135.t1 | 28744922 | 28746187 |
| chr_2 | g8135 | g8135.t1 | exon | g8135.t1.exon1 | 28744922 | 28745119 |
| chr_2 | g8135 | g8135.t1 | cds | g8135.t1.CDS1 | 28744922 | 28745119 |
| chr_2 | g8135 | g8135.t1 | exon | g8135.t1.exon2 | 28745186 | 28745283 |
| chr_2 | g8135 | g8135.t1 | cds | g8135.t1.CDS2 | 28745186 | 28745283 |
| chr_2 | g8135 | g8135.t1 | exon | g8135.t1.exon3 | 28745353 | 28746187 |
| chr_2 | g8135 | g8135.t1 | cds | g8135.t1.CDS3 | 28745353 | 28746187 |
| chr_2 | g8135 | g8135.t1 | TTS | g8135.t1 | 28746322 | 28746322 |
Sequences
>g8135.t1 Gene=g8135 Length=1131
ATGAATGAAGATAAAATAAGTTTTCTGAGAAAACATGTTGTTATTATCAATTATGTCCGA
TCATTTTTAAAATCAATTAAATTTGCACCAATTAATGTGCCTTTTAATCAAAGATGTGAA
GTTTTTAGCGCTTTTCTATTTATTTCAATGGTCTTGTTTGCTGAAGCATTATGTGTTCTA
ATCATTTATTTTTTACTCTTTCATGGAGGTTTAGTGGGCCAAATATTTTGTATCGGGTAT
TTTGCATTTATGATAAGTGATATAAAAGCAGATGAAACTGGAATAAGAGGTCAAGGATCA
AAATGGGTGCGCTCTTGGAAATGGTGGAAATATTATGCAAACTATTTTCCTGTTAAACTC
ATCAAAACTGTTGATCTTCCACCAAATAAAAATTATTTATTTGGATGTTTTCCGCATGCT
ATCATCGGATGCGGTCTTTTTGCAAATTTTGCAACAAATGCGACAGGATTTAATGAAAAT
TTTCCTGGAATAAGGTCAAAAATGTGTACACTTTCATTTCATTTTTATGTGCCATTTTTT
AGAGAGCTTGCTTTCTCGTGGGGTCTAATGTCAGCAAAGTATGTTTCTATAAAACAAGCT
CTCTCTCAATCAACAAATCCTTTAGCAGTAGCAAATCAAGCTGATAAATTTACGAGTAAT
GCTGTAGTTTTGGTAGTTGGTGGAGCTCAAGAAGCACTAATGTCTCATCCTGGGAAATAT
GAAATTTTTATAAAAAGTCGCAAAGGATTTATCAAAATTGCGCTTGAAACTGGAGCATCT
CTTGTACCGGTCTTCTCTTTTGGAGAAACCGAGGTTTATGATCAAACACCAAATGAACCA
GGGAGCAAAGTTAGAAGGTTTCAGGAAGCATTTAAAAAATGGACGGGTGTCGCTCCCGCA
ATTTTTATTGGTCGAGGATTTTTTCAATATTCTTTTGGTCTCATTCCACGACGTGCTCCG
ATTCATACCATTGTTGGTGCACCTATTGCTGTCGAAAAGATTTTATCACCTTCAAAAGAG
GATATTGATAATTTGCATCAAAAATTCATTGAAGAACTTGAGAAATTATTTGAAGAGCAT
AAAAGAAAATATATTCCAAATGCTGAAAATGTTAAATTGATTATGAGATAA
>g8135.t1 Gene=g8135 Length=376
MNEDKISFLRKHVVIINYVRSFLKSIKFAPINVPFNQRCEVFSAFLFISMVLFAEALCVL
IIYFLLFHGGLVGQIFCIGYFAFMISDIKADETGIRGQGSKWVRSWKWWKYYANYFPVKL
IKTVDLPPNKNYLFGCFPHAIIGCGLFANFATNATGFNENFPGIRSKMCTLSFHFYVPFF
RELAFSWGLMSAKYVSIKQALSQSTNPLAVANQADKFTSNAVVLVVGGAQEALMSHPGKY
EIFIKSRKGFIKIALETGASLVPVFSFGETEVYDQTPNEPGSKVRRFQEAFKKWTGVAPA
IFIGRGFFQYSFGLIPRRAPIHTIVGAPIAVEKILSPSKEDIDNLHQKFIEELEKLFEEH
KRKYIPNAENVKLIMR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g8135.t1 | CDD | cd07987 | LPLAT_MGAT-like | 112 | 362 | 3.8311E-52 |
| 5 | g8135.t1 | Coils | Coil | Coil | 339 | 359 | - |
| 2 | g8135.t1 | PANTHER | PTHR12317 | DIACYLGLYCEROL O-ACYLTRANSFERASE | 25 | 372 | 2.7E-110 |
| 3 | g8135.t1 | PANTHER | PTHR12317:SF0 | DIACYLGLYCEROL O-ACYLTRANSFERASE 1 | 25 | 372 | 2.7E-110 |
| 1 | g8135.t1 | Pfam | PF03982 | Diacylglycerol acyltransferase | 79 | 366 | 1.6E-100 |
| 7 | g8135.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 40 | - |
| 10 | g8135.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 41 | 65 | - |
| 8 | g8135.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 66 | 70 | - |
| 9 | g8135.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 71 | 88 | - |
| 6 | g8135.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 89 | 376 | - |
| 4 | g8135.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 45 | 67 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016747 | acyltransferase activity, transferring groups other than amino-acyl groups | MF |
KEGG
Orthology
- This transcript has been annotated with K14457 (2-acylglycerol O-acyltransferase 2)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
