General information for g8135.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative 2-acylglycerol O-acyltransferase 1.
Parent gene
- The parent gene g8135 is located on chr_2: 28746187..28747699 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8135 | g8135.t5 | TSS | g8135.t5 | 28744788 | 28744788 |
| chr_2 | g8135 | g8135.t5 | isoform | g8135.t5 | 28744922 | 28746187 |
| chr_2 | g8135 | g8135.t5 | exon | g8135.t5.exon1 | 28744922 | 28745294 |
| chr_2 | g8135 | g8135.t5 | exon | g8135.t5.exon2 | 28745353 | 28746187 |
| chr_2 | g8135 | g8135.t5 | cds | g8135.t5.CDS1 | 28745558 | 28746187 |
| chr_2 | g8135 | g8135.t5 | TTS | g8135.t5 | 28746322 | 28746322 |
Sequences
>g8135.t5 Gene=g8135 Length=1208
ATGAATGAAGATAAAATAAGTTTTCTGAGAAAACATGTTGTTATTATCAATTATGTCCGA
TCATTTTTAAAATCAATTAAATTTGCACCAATTAATGTGCCTTTTAATCAAAGATGTGAA
GTTTTTAGCGCTTTTCTATTTATTTCAATGGTCTTGTTTGCTGAAGCATTATGTGTTCTA
ATCATTTATTTTTTACTCGTGAGTTTTAAATTTCATTTTTTAAAATATTTTAATTGAATC
AGGATTTTATTTTCATTTATTTAGTTTCATGGAGGTTTAGTGGGCCAAATATTTTGTATC
GGGTATTTTGCATTTATGATAAGTGATATAAAAGCAGATGAAACTGGAATAAGAGGTCAA
GGGTAAATTTAAAATCAAAATGGGTGCGCTCTTGGAAATGGTGGAAATATTATGCAAACT
ATTTTCCTGTTAAACTCATCAAAACTGTTGATCTTCCACCAAATAAAAATTATTTATTTG
GATGTTTTCCGCATGCTATCATCGGATGCGGTCTTTTTGCAAATTTTGCAACAAATGCGA
CAGGATTTAATGAAAATTTTCCTGGAATAAGGTCAAAAATGTGTACACTTTCATTTCATT
TTTATGTGCCATTTTTTAGAGAGCTTGCTTTCTCGTGGGGTCTAATGTCAGCAAAGTATG
TTTCTATAAAACAAGCTCTCTCTCAATCAACAAATCCTTTAGCAGTAGCAAATCAAGCTG
ATAAATTTACGAGTAATGCTGTAGTTTTGGTAGTTGGTGGAGCTCAAGAAGCACTAATGT
CTCATCCTGGGAAATATGAAATTTTTATAAAAAGTCGCAAAGGATTTATCAAAATTGCGC
TTGAAACTGGAGCATCTCTTGTACCGGTCTTCTCTTTTGGAGAAACCGAGGTTTATGATC
AAACACCAAATGAACCAGGGAGCAAAGTTAGAAGGTTTCAGGAAGCATTTAAAAAATGGA
CGGGTGTCGCTCCCGCAATTTTTATTGGTCGAGGATTTTTTCAATATTCTTTTGGTCTCA
TTCCACGACGTGCTCCGATTCATACCATTGTTGGTGCACCTATTGCTGTCGAAAAGATTT
TATCACCTTCAAAAGAGGATATTGATAATTTGCATCAAAAATTCATTGAAGAACTTGAGA
AATTATTTGAAGAGCATAAAAGAAAATATATTCCAAATGCTGAAAATGTTAAATTGATTA
TGAGATAA
>g8135.t5 Gene=g8135 Length=209
MCTLSFHFYVPFFRELAFSWGLMSAKYVSIKQALSQSTNPLAVANQADKFTSNAVVLVVG
GAQEALMSHPGKYEIFIKSRKGFIKIALETGASLVPVFSFGETEVYDQTPNEPGSKVRRF
QEAFKKWTGVAPAIFIGRGFFQYSFGLIPRRAPIHTIVGAPIAVEKILSPSKEDIDNLHQ
KFIEELEKLFEEHKRKYIPNAENVKLIMR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g8135.t5 | CDD | cd07987 | LPLAT_MGAT-like | 2 | 195 | 2.25268E-42 |
| 4 | g8135.t5 | Coils | Coil | Coil | 172 | 192 | - |
| 2 | g8135.t5 | PANTHER | PTHR12317 | DIACYLGLYCEROL O-ACYLTRANSFERASE | 2 | 205 | 2.5E-69 |
| 3 | g8135.t5 | PANTHER | PTHR12317:SF0 | DIACYLGLYCEROL O-ACYLTRANSFERASE 1 | 2 | 205 | 2.5E-69 |
| 1 | g8135.t5 | Pfam | PF03982 | Diacylglycerol acyltransferase | 1 | 199 | 4.6E-66 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016747 | acyltransferase activity, transferring groups other than amino-acyl groups | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
