General information for g8136.t22
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Brachyurin.
Parent gene
- The parent gene g8136 is located on chr_2: 28746241..28747343 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8136 | g8136.t22 | TTS | g8136.t22 | 28746198 | 28746198 |
| chr_2 | g8136 | g8136.t22 | isoform | g8136.t22 | 28746241 | 28747343 |
| chr_2 | g8136 | g8136.t22 | exon | g8136.t22.exon1 | 28746241 | 28746298 |
| chr_2 | g8136 | g8136.t22 | exon | g8136.t22.exon2 | 28746358 | 28746457 |
| chr_2 | g8136 | g8136.t22 | exon | g8136.t22.exon3 | 28746530 | 28747242 |
| chr_2 | g8136 | g8136.t22 | cds | g8136.t22.CDS1 | 28746743 | 28747242 |
| chr_2 | g8136 | g8136.t22 | exon | g8136.t22.exon4 | 28747310 | 28747343 |
| chr_2 | g8136 | g8136.t22 | cds | g8136.t22.CDS2 | 28747310 | 28747343 |
| chr_2 | g8136 | g8136.t22 | TSS | g8136.t22 | 28747477 | 28747477 |
Sequences
>g8136.t22 Gene=g8136 Length=905
ATGACTAATTTTCGTAGTGGAAGTAGAATTGCTGGTGGAACAGAAGCATCGCGAAATCAA
TTCCCATTTGCCACAATTTTCTTTATTTATGCTCCAAACGATGATCAATCAATTTGTTCT
GGATCAATTCTTTCAGCTCATTATGTTCTATCAGCAGCTCATTGCTTTACAAATTTAGCT
TCTGCCGATTTACTTGCAGGTGTGCACAATGTTGAAACAGAATTTCCCATATACGAGCTA
ACAATATTCAACTCTGACATTATCATTCATGAATCGTACGACAGAGTTCGTTATACAAAT
GATATTGCAGTTATAAGCACAAGACGATCACCATTTTCATTCACAAGAATTGAAATTCAA
CCAATTAATATCATCCCAAGATCATATGCAAATAGTAATCTTACTGGATATATGGCTAGA
ATTTCAGGATGGGGCCTAACCAGTGATACATCATCCATTAGTCCCGTGCCAAGATATATT
GATGCACCTATCATATCAAATGGTAAACTAAAATTAGTTTTATTAATTTTTTAATTCAAA
AAATTGAATCATGACTCATAAAGGTCTTTTCAAAAAATCCAAATGATAAAATCTGTGCTT
GACACACTATCAAGTTTGTTTCAAATTTCGGCTTTTTTTCTTTTTGTAAAGATGAGTGTG
CGAGAACATTCGGTACATTGATAACGAGTGGAAACATTTGTCTGAGTGGTAGCAATGGCA
GATCGACATGTCCAGGGGACAGCGGTGGTGGAATGACAATAGCTCATGGTAGTACACGAG
TCCTTGCTGGAATTGTTAGTTTCGGAAGCGATAGAGGATGTACATTAGGCTATCCAACTG
CCAAAACACGTATTACTGCATTTTATGATTGGATTACAAGTAAAACTGGAATTGTGATTT
CATAA
>g8136.t22 Gene=g8136 Length=177
MTNFRSGSRIAGGTEASRNQFPFATIFFIYAPNDDQSICSGSILSAHYVLSAAHCFTNLA
SADLLAGVHNVETEFPIYELTIFNSDIIIHESYDRVRYTNDIAVISTRRSPFSFTRIEIQ
PINIIPRSYANSNLTGYMARISGWGLTSDTSSISPVPRYIDAPIISNGKLKLVLLIF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g8136.t22 | Gene3D | G3DSA:2.40.10.10 | - | 3 | 173 | 3.5E-29 |
| 2 | g8136.t22 | PANTHER | PTHR24250:SF56 | SERINE PROTEASE P96 | 5 | 170 | 1.6E-27 |
| 3 | g8136.t22 | PANTHER | PTHR24250 | CHYMOTRYPSIN-RELATED | 5 | 170 | 1.6E-27 |
| 1 | g8136.t22 | Pfam | PF00089 | Trypsin | 10 | 169 | 1.4E-19 |
| 6 | g8136.t22 | ProSitePatterns | PS00134 | Serine proteases, trypsin family, histidine active site. | 50 | 55 | - |
| 8 | g8136.t22 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 10 | 177 | 14.375 |
| 5 | g8136.t22 | SMART | SM00020 | trypsin_2 | 9 | 176 | 8.6E-7 |
| 4 | g8136.t22 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 7 | 171 | 3.93E-34 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed