General information for g8147.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein will die slowly.
Parent gene
- The parent gene g8147 is located on chr_2: 28791269..28792215 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8147 | g8147.t1 | TTS | g8147.t1 | 28790529 | 28790529 |
| chr_2 | g8147 | g8147.t1 | isoform | g8147.t1 | 28790901 | 28792215 |
| chr_2 | g8147 | g8147.t1 | exon | g8147.t1.exon1 | 28790901 | 28791089 |
| chr_2 | g8147 | g8147.t1 | cds | g8147.t1.CDS1 | 28790901 | 28791089 |
| chr_2 | g8147 | g8147.t1 | exon | g8147.t1.exon2 | 28791147 | 28791481 |
| chr_2 | g8147 | g8147.t1 | cds | g8147.t1.CDS2 | 28791147 | 28791481 |
| chr_2 | g8147 | g8147.t1 | exon | g8147.t1.exon3 | 28791540 | 28791830 |
| chr_2 | g8147 | g8147.t1 | cds | g8147.t1.CDS3 | 28791540 | 28791830 |
| chr_2 | g8147 | g8147.t1 | exon | g8147.t1.exon4 | 28791915 | 28792011 |
| chr_2 | g8147 | g8147.t1 | cds | g8147.t1.CDS4 | 28791915 | 28792011 |
| chr_2 | g8147 | g8147.t1 | exon | g8147.t1.exon5 | 28792075 | 28792215 |
| chr_2 | g8147 | g8147.t1 | cds | g8147.t1.CDS5 | 28792075 | 28792215 |
| chr_2 | g8147 | g8147.t1 | TSS | g8147.t1 | 28792448 | 28792448 |
Sequences
>g8147.t1 Gene=g8147 Length=1053
ATGATGATGCCACATCCTTCGCAACTACAAGGCCATCCAATGCATCCTGGAGCAATGCAA
CAAATGCAAGTAGCTCCACCACCGTCACAAGCTCAACAACAGCCTCAGCAACAATCTTCA
AATCGTCAATCGGCTTCACTTAAACCAAATTATACGCTAAAATTTACTTTAGCTGGTCAC
ACAAAAGCAGTTTCTGCTGTAAAGTTCAGTCCAAACGGTGAATGGCTTGCGAGTTCTTCT
GCTGATAAACTTATTAAAATTTGGGGAGCTTATGATGGTAAATTTGAAAAAACAATAGCA
GGTCACAAATTAGGAATTAGCGATGTAGCTTGGAGTAGTGACAGCCGATTGCTGGTTACA
GCTAGTGATGACAAAACATTGAAAATTTGGGAATTAAGCAGTGGAAAATGTCTGAAAACG
CTCAAAGGACACAGCAACTATGTATTTTGTTGTAATTTCAATCCGCAGAGTAATCTAATC
GTCTCTGGAAGTTTCGATGAGTCTGTGAGAATTTGGGATGTAAGAACAGGAAAATGTTTG
AAAACTTTACCAGCACATTCTGATCCTGTTTCAGCAGTTCATTTTAATCGTGATGGTTCA
TTAATTGTATCCTCGAGCTATGATGGACTTTGTCGTATTTGGGATACAGCTAGTGGTCAA
TGTTTAAAAACATTAATTGACGATGATAATCCTCCAGTGTCGTTTGTTAAATTTTCTCCA
AATGGAAAATACATTCTCGCAGCTACGTTGGATAACACCCTCAAATTGTGGGATTATTCG
AAAGGAAAATGTCTCAAAACATACACAGGACACAAAAACGAGAGATATTGTATTTTCGCA
AACTTTTCAGTAACTGGAGGAAAGTGGATTGTATCTGGAAGTGAAGATAATATGGTTTAT
ATCTGGAATCTTCAAAGCAAAGAGATTGTACAACGTTTACAGGGACATACTGATGTGGTC
CTGTGCACAGCCTGTCACCCAACTGAAAATATCATAGCATCTGCTGCACTCGAAAATGAC
AAAACCATAAAATTATGGAAGAGTGATACATAA
>g8147.t1 Gene=g8147 Length=350
MMMPHPSQLQGHPMHPGAMQQMQVAPPPSQAQQQPQQQSSNRQSASLKPNYTLKFTLAGH
TKAVSAVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTIAGHKLGISDVAWSSDSRLLVT
ASDDKTLKIWELSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVRTGKCL
KTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSP
NGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNERYCIFANFSVTGGKWIVSGSEDNMVY
IWNLQSKEIVQRLQGHTDVVLCTACHPTENIIASAALENDKTIKLWKSDT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 16 | g8147.t1 | CDD | cd00200 | WD40 | 53 | 347 | 1.66316E-106 |
| 14 | g8147.t1 | Gene3D | G3DSA:2.130.10.10 | - | 36 | 350 | 7.0E-154 |
| 29 | g8147.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 20 | 45 | - |
| 28 | g8147.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 23 | 45 | - |
| 8 | g8147.t1 | PANTHER | PTHR22847 | WD40 REPEAT PROTEIN | 29 | 349 | 3.7E-195 |
| 9 | g8147.t1 | PANTHER | PTHR22847:SF560 | WD REPEAT-CONTAINING PROTEIN 5 | 29 | 349 | 3.7E-195 |
| 15 | g8147.t1 | PIRSF | PIRSF002394 | GNBP_B | 13 | 349 | 4.4E-93 |
| 10 | g8147.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 118 | 132 | 1.3E-8 |
| 12 | g8147.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 160 | 174 | 1.3E-8 |
| 11 | g8147.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 290 | 304 | 1.3E-8 |
| 3 | g8147.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 55 | 88 | 1.1E-8 |
| 2 | g8147.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 96 | 130 | 2.8E-7 |
| 5 | g8147.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 135 | 173 | 5.4E-10 |
| 7 | g8147.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 177 | 215 | 4.9E-9 |
| 1 | g8147.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 219 | 258 | 2.2E-5 |
| 6 | g8147.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 262 | 303 | 1.8E-5 |
| 4 | g8147.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 310 | 347 | 0.0045 |
| 24 | g8147.t1 | ProSitePatterns | PS00678 | Trp-Asp (WD) repeats signature. | 118 | 132 | - |
| 26 | g8147.t1 | ProSitePatterns | PS00678 | Trp-Asp (WD) repeats signature. | 160 | 174 | - |
| 27 | g8147.t1 | ProSitePatterns | PS00678 | Trp-Asp (WD) repeats signature. | 202 | 216 | - |
| 25 | g8147.t1 | ProSitePatterns | PS00678 | Trp-Asp (WD) repeats signature. | 290 | 304 | - |
| 30 | g8147.t1 | ProSiteProfiles | PS50294 | Trp-Asp (WD) repeats circular profile. | 57 | 350 | 74.137 |
| 34 | g8147.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 57 | 98 | 15.655 |
| 31 | g8147.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 99 | 140 | 17.159 |
| 33 | g8147.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 141 | 182 | 18.195 |
| 36 | g8147.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 183 | 224 | 15.956 |
| 35 | g8147.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 226 | 267 | 13.917 |
| 32 | g8147.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 268 | 312 | 10.341 |
| 22 | g8147.t1 | SMART | SM00320 | WD40_4 | 50 | 89 | 5.5E-10 |
| 23 | g8147.t1 | SMART | SM00320 | WD40_4 | 92 | 131 | 5.1E-10 |
| 19 | g8147.t1 | SMART | SM00320 | WD40_4 | 134 | 173 | 1.6E-12 |
| 18 | g8147.t1 | SMART | SM00320 | WD40_4 | 176 | 215 | 3.2E-12 |
| 17 | g8147.t1 | SMART | SM00320 | WD40_4 | 218 | 258 | 1.3E-6 |
| 20 | g8147.t1 | SMART | SM00320 | WD40_4 | 261 | 303 | 3.4E-5 |
| 21 | g8147.t1 | SMART | SM00320 | WD40_4 | 306 | 347 | 1.2E-4 |
| 13 | g8147.t1 | SUPERFAMILY | SSF50978 | WD40 repeat-like | 49 | 347 | 1.83E-102 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
- This transcript has been annotated with K14963 (COMPASS component SWD3)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
