General information for g8177.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Motor neuron and pancreas homeobox protein 1.
Parent gene
- The parent gene g8177 is located on chr_2: 28927137..28928716 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8177 | g8177.t1 | TTS | g8177.t1 | 28927069 | 28927069 |
| chr_2 | g8177 | g8177.t1 | isoform | g8177.t1 | 28927137 | 28928716 |
| chr_2 | g8177 | g8177.t1 | exon | g8177.t1.exon1 | 28927137 | 28927355 |
| chr_2 | g8177 | g8177.t1 | cds | g8177.t1.CDS1 | 28927137 | 28927355 |
| chr_2 | g8177 | g8177.t1 | exon | g8177.t1.exon2 | 28927995 | 28928099 |
| chr_2 | g8177 | g8177.t1 | cds | g8177.t1.CDS2 | 28927995 | 28928099 |
| chr_2 | g8177 | g8177.t1 | exon | g8177.t1.exon3 | 28928167 | 28928216 |
| chr_2 | g8177 | g8177.t1 | cds | g8177.t1.CDS3 | 28928167 | 28928216 |
| chr_2 | g8177 | g8177.t1 | exon | g8177.t1.exon4 | 28928276 | 28928490 |
| chr_2 | g8177 | g8177.t1 | cds | g8177.t1.CDS4 | 28928276 | 28928490 |
| chr_2 | g8177 | g8177.t1 | exon | g8177.t1.exon5 | 28928547 | 28928716 |
| chr_2 | g8177 | g8177.t1 | cds | g8177.t1.CDS5 | 28928547 | 28928716 |
| chr_2 | g8177 | g8177.t1 | TSS | g8177.t1 | 28928843 | 28928843 |
Sequences
>g8177.t1 Gene=g8177 Length=759
ATGAAGGGTGAAGATTTAAGTGATATACATATACCAGAAAATATAATTTATCGTAATAAT
AGTAATCAAAAACCAATTAAAAGTTCATTTTTAATTGAGTCATTATTATCAAATAATAAT
GCTAAGCAAAAAGATGATTTAATGAAAAAAGATCAAGATAATTGTGGAAGTGATTCAAAT
TCGTGCAACTCGTCAATATGTTCAAGAAGTCCATCAATTAGTCCTGGATGTGAAAATTCA
GAGAGTAGTGGCATAAGACATTTTGCAGATATGACACAACTTCCAATTTCTTCACACCCA
CTTATGAGTTTTCATTCGCATTATCCAAATTTAGAAATGTTTTATCATCCACGACAATTT
TATTACGACAACTTTGACTTCAATAGGCATCCAGGATTATTTGGAAAAACTCGTAGACCA
CGTACTGCATTTACGTCACAACAACTTTTGGAACTCGAAAAGCAATTTAAACAGAATAAA
TATCTATCACGTCCTAAACGATATGAAGTTGCAAGCAATCTACTACTGACAGAAACTCAG
GTGAAGATTTGGTTTCAAAATCGAAGAATGAAGTTTAAACGATCAAGGAGAACACAAAAA
GAGCATGCCACTGCCGATAAAGAACGAAAGAATTCAATAAACAGTCAAAAAACACAGGAT
GATCTCAAGTCATCAAAAAATGATCTCAACAATAAATCACTACCTTTCCAACATTCACAT
CCTCACACTTATGATTCATATGTTCCTAGTTTAGCATAG
>g8177.t1 Gene=g8177 Length=252
MKGEDLSDIHIPENIIYRNNSNQKPIKSSFLIESLLSNNNAKQKDDLMKKDQDNCGSDSN
SCNSSICSRSPSISPGCENSESSGIRHFADMTQLPISSHPLMSFHSHYPNLEMFYHPRQF
YYDNFDFNRHPGLFGKTRRPRTAFTSQQLLELEKQFKQNKYLSRPKRYEVASNLLLTETQ
VKIWFQNRRMKFKRSRRTQKEHATADKERKNSINSQKTQDDLKSSKNDLNNKSLPFQHSH
PHTYDSYVPSLA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g8177.t1 | CDD | cd00086 | homeodomain | 138 | 196 | 1.01845E-20 |
| 7 | g8177.t1 | Gene3D | G3DSA:1.10.10.60 | - | 119 | 198 | 4.8E-25 |
| 11 | g8177.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 194 | 252 | - |
| 13 | g8177.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 199 | 227 | - |
| 12 | g8177.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 228 | 244 | - |
| 2 | g8177.t1 | PANTHER | PTHR24335 | MOTOR NEURON AND PANCREAS HOMEOBOX PROTEIN | 75 | 241 | 1.6E-43 |
| 5 | g8177.t1 | PRINTS | PR00024 | Homeobox signature | 159 | 170 | 5.3E-8 |
| 4 | g8177.t1 | PRINTS | PR00024 | Homeobox signature | 174 | 184 | 5.3E-8 |
| 3 | g8177.t1 | PRINTS | PR00024 | Homeobox signature | 184 | 193 | 5.3E-8 |
| 1 | g8177.t1 | Pfam | PF00046 | Homeodomain | 138 | 194 | 2.8E-21 |
| 10 | g8177.t1 | ProSitePatterns | PS00027 | ‘Homeobox’ domain signature. | 170 | 193 | - |
| 14 | g8177.t1 | ProSiteProfiles | PS50071 | ‘Homeobox’ domain profile. | 135 | 195 | 21.994 |
| 9 | g8177.t1 | SMART | SM00389 | HOX_1 | 137 | 199 | 3.5E-24 |
| 6 | g8177.t1 | SUPERFAMILY | SSF46689 | Homeodomain-like | 123 | 195 | 1.5E-23 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0021520 | spinal cord motor neuron cell fate specification | BP |
| GO:0003677 | DNA binding | MF |
| GO:0000981 | DNA-binding transcription factor activity, RNA polymerase II-specific | MF |
| GO:0006355 | regulation of transcription, DNA-templated | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed