General information for g8184.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial.
Parent gene
- The parent gene g8184 is located on chr_2: 28961709..28964626 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8184 | g8184.t2 | TSS | g8184.t2 | 28959387 | 28959387 |
| chr_2 | g8184 | g8184.t2 | isoform | g8184.t2 | 28959488 | 28960794 |
| chr_2 | g8184 | g8184.t2 | exon | g8184.t2.exon1 | 28959488 | 28959559 |
| chr_2 | g8184 | g8184.t2 | cds | g8184.t2.CDS1 | 28959488 | 28959559 |
| chr_2 | g8184 | g8184.t2 | exon | g8184.t2.exon2 | 28959644 | 28959773 |
| chr_2 | g8184 | g8184.t2 | cds | g8184.t2.CDS2 | 28959644 | 28959773 |
| chr_2 | g8184 | g8184.t2 | exon | g8184.t2.exon3 | 28959840 | 28959923 |
| chr_2 | g8184 | g8184.t2 | cds | g8184.t2.CDS3 | 28959840 | 28959923 |
| chr_2 | g8184 | g8184.t2 | exon | g8184.t2.exon4 | 28959981 | 28960117 |
| chr_2 | g8184 | g8184.t2 | cds | g8184.t2.CDS4 | 28959981 | 28960117 |
| chr_2 | g8184 | g8184.t2 | exon | g8184.t2.exon5 | 28960172 | 28960794 |
| chr_2 | g8184 | g8184.t2 | cds | g8184.t2.CDS5 | 28960172 | 28960792 |
| chr_2 | g8184 | g8184.t2 | TTS | g8184.t2 | NA | NA |
Sequences
>g8184.t2 Gene=g8184 Length=1046
ATGATTTCAAAGTGTTTAAGTTCTTTATTAAAACAAAAAGCAATAGGAATAAACTCAAAT
TACCATCAAAAGATATTAGGCTGCTATTTTTCAATCAGCGCCAGACATAACTCGAAAGCC
TACAAAGGAAAAATTTATTTGAATGCTAAAGATGCAGTCGCTGACATACCCGATGGTGCA
AAAATCTTGGTGGGAGGCTTCGGTTTATGTGGAATTCCTGAGAACCTTATTCAAGGTTTA
TTAGATATTGGCATCAAAGATTTAACAGTTGTCTCGAATAATGCAGGCGTTGATGATTTT
GGACTTGGTCTTCTTCTCAAAGACCGTCGAATTAAACGCATGATAGCATCATATGTCGGC
GAAAATGCAGAATTCGAAAGACAATATTTATCTGGTGACTTAGAACTTGAATTGACTCCA
CAAGGGACATTAGCGGAAAGAATCAGAGCTGGTGGAGCAGGTATTCCTGCATTTTTCACA
CCTACTGGTACTTTTACATTGATTCATGAAGGGGGAAGTCCTATTAAATATGTTAAGGGT
AAAAATAAGGAAGTTGAGATTGCAAGTGCAAAAAGAGAAATGAGAGAATTTAATGGAAAA
CCTTATATCATGGAGGAAGCAATTACTGGCGACTTTGCATTAATTCGTGCTTATAAGGCA
GATGAACTTGGAAATTTGATATTTAGAAAATCAGCTAGAAATTTTAATCCACCTATGGCA
AAAGCAGCTAAAATTACAATTGCAGAAGTTGAAGAAATTGTTCCAATTGGTGCACTTCAA
CCTGATGAAATTCATCTACCTGGTATTTTTGTGCATCGCATTATTCGCGGAAAATTTGAA
AAGAGAATTGAAAGAATAAAAATTACAAAACCAGAAACTAATGAGAAAAAACTGTCAAAA
GAAGCACTACTTCGAGAAAGAATCGTTCGTCGTGTTGCTTGCGAATATAAAGACGGAATG
TATATCAATTTAGGAATTGGAATTCCTGTTTTATCAAGCAATTATATACCTAAAGATATT
ACAGTTCATCTTCAATCAGAAAATGG
>g8184.t2 Gene=g8184 Length=348
MISKCLSSLLKQKAIGINSNYHQKILGCYFSISARHNSKAYKGKIYLNAKDAVADIPDGA
KILVGGFGLCGIPENLIQGLLDIGIKDLTVVSNNAGVDDFGLGLLLKDRRIKRMIASYVG
ENAEFERQYLSGDLELELTPQGTLAERIRAGGAGIPAFFTPTGTFTLIHEGGSPIKYVKG
KNKEVEIASAKREMREFNGKPYIMEEAITGDFALIRAYKADELGNLIFRKSARNFNPPMA
KAAKITIAEVEEIVPIGALQPDEIHLPGIFVHRIIRGKFEKRIERIKITKPETNEKKLSK
EALLRERIVRRVACEYKDGMYINLGIGIPVLSSNYIPKDITVHLQSEN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g8184.t2 | Gene3D | G3DSA:3.40.1080.10 | - | 39 | 277 | 1.5E-106 |
| 7 | g8184.t2 | Gene3D | G3DSA:3.40.1080.10 | - | 300 | 348 | 7.1E-14 |
| 2 | g8184.t2 | PANTHER | PTHR13707 | KETOACID-COENZYME A TRANSFERASE | 21 | 348 | 5.8E-144 |
| 3 | g8184.t2 | PANTHER | PTHR13707:SF30 | SUCCINYL-COA:3-KETOACID COENZYME A TRANSFERASE 1, MITOCHONDRIAL | 21 | 348 | 5.8E-144 |
| 1 | g8184.t2 | Pfam | PF01144 | Coenzyme A transferase | 48 | 275 | 7.3E-77 |
| 6 | g8184.t2 | ProSitePatterns | PS01273 | Coenzyme A transferases signature 1. | 58 | 73 | - |
| 5 | g8184.t2 | SMART | SM00882 | CoA_trans_3 | 46 | 277 | 6.4E-91 |
| 4 | g8184.t2 | SUPERFAMILY | SSF100950 | NagB/RpiA/CoA transferase-like | 44 | 348 | 2.96E-93 |
| 9 | g8184.t2 | TIGRFAM | TIGR02429 | pcaI_scoA_fam: 3-oxoacid CoA-transferase, A subunit | 44 | 186 | 5.7E-44 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008410 | CoA-transferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01028 (3-oxoacid CoA-transferase subunit A)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed