General information for g8185.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as hypothetical.
Parent gene
- The parent gene g8185 is located on chr_2: 28963057..28964797 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8185 | g8185.t10 | isoform | g8185.t10 | 28961709 | 28963602 |
| chr_2 | g8185 | g8185.t10 | exon | g8185.t10.exon1 | 28961709 | 28962247 |
| chr_2 | g8185 | g8185.t10 | TTS | g8185.t10 | 28961757 | 28961757 |
| chr_2 | g8185 | g8185.t10 | cds | g8185.t10.CDS1 | 28962204 | 28962247 |
| chr_2 | g8185 | g8185.t10 | exon | g8185.t10.exon2 | 28962309 | 28962702 |
| chr_2 | g8185 | g8185.t10 | cds | g8185.t10.CDS2 | 28962309 | 28962702 |
| chr_2 | g8185 | g8185.t10 | exon | g8185.t10.exon3 | 28962761 | 28962952 |
| chr_2 | g8185 | g8185.t10 | cds | g8185.t10.CDS3 | 28962761 | 28962952 |
| chr_2 | g8185 | g8185.t10 | exon | g8185.t10.exon4 | 28963542 | 28963602 |
| chr_2 | g8185 | g8185.t10 | cds | g8185.t10.CDS4 | 28963542 | 28963568 |
| chr_2 | g8185 | g8185.t10 | TSS | g8185.t10 | 28963786 | 28963786 |
Sequences
>g8185.t10 Gene=g8185 Length=1186
ATGGGTTTAACTTGTGGAGCATCTGTGGTCAAATATGGCATTTTTATTTTCAACCTTCTT
TGTTGCTGGTATAGCATTGATCGTTGTTGGTGCAATTCCACTATTCAAACTTGATGACAT
TCGAGATGCCTTTCCTGAAAACAATCCAGCCACTGTTCCCATCATAATCGTCGTTCTTGG
CTCGATAATCTTTATCATCAGTTTCTTTGGATGTTGTGGAGCTATTAGAGAAAATCAGTG
TATGGTTTCAACGTACGCATTTTTCCTACTCATATTGGTCGTTCTTCAAATTGTTATCGC
CGTTTTCGCATTTATGTATACAGGTGAACTTGCGAAAGCTGCAGAAAATGGTTTCAATAA
ATTATGGGAAAACAGAGACACAAATAATGAAACAAGAATTGCTATCGAAGGCATACAACG
CGGACTTCGCTGTTGTGGTTCAGCTGGACCCGCTGATTGGGGATTATTAAATATTCCATC
TTCATGCTGTGATGATGGAGTTATAGCATGCACAGTGTCGAACGCTCATTCAGATGGATG
TGCTACACTTCTTTCCAACTTTGTCAATGATAGTGGACTCTTGATTGCATGGTTTGCAAT
TGTCTTTGCTGGTATTGAACTTGTTGGTGTAATCTTCGCCTGTTGTCTGGCCAATTCAAT
TCGAAATGCAAACAGAAGACAATATGCATAAAAGTCTACAATGTACTAATTTAATAGAAA
TAGGAATTTGAAACCAAATTTTTTTTGTTTAAAAATCTATTCTTATATATCTCTAATTAA
TTTTATAGTACAGCTATAGATTGATGTCAAGAAAATAAGTTTTTTTTTCACTTATTTATC
GCTATTAATTGATAAGTAAGTTGAATACATATGTGTGTGTGTCACCATTTCTTTGGTCTA
TCATCTATATCAATTCTAATTTATCAATCTTAAGTTTCTGTGTGAGCTAGGCACGGCAGA
TAAGGGTCACTTCTATATATGCAAGAGAAAAGAAAACACGCCAACAAGAAATATTTTATT
TTTATTTTAGTCTTTTAAAATATGTTATCGTAAAAATTGTATATTTTTTATTCTTTAAAT
GTTGTACTTTGAACACAAGTTGTTAATAAACAAAGTAAAAGTGATTAGTTCTTGAATAAA
TGCTTTAAATATCAACAATTCAAAATTTAATGGTAAATAATAATTT
>g8185.t10 Gene=g8185 Length=218
MAFLFSTFFVAGIALIVVGAIPLFKLDDIRDAFPENNPATVPIIIVVLGSIIFIISFFGC
CGAIRENQCMVSTYAFFLLILVVLQIVIAVFAFMYTGELAKAAENGFNKLWENRDTNNET
RIAIEGIQRGLRCCGSAGPADWGLLNIPSSCCDDGVIACTVSNAHSDGCATLLSNFVNDS
GLLIAWFAIVFAGIELVGVIFACCLANSIRNANRRQYA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 21 | g8185.t10 | CDD | cd03127 | tetraspanin_LEL | 93 | 179 | 1.21548E-18 |
| 8 | g8185.t10 | Gene3D | G3DSA:1.10.1450.10 | Tetraspanin | 96 | 178 | 8.5E-13 |
| 2 | g8185.t10 | PANTHER | PTHR19282 | TETRASPANIN | 3 | 212 | 3.5E-39 |
| 3 | g8185.t10 | PANTHER | PTHR19282:SF471 | CD63 ANTIGEN | 3 | 212 | 3.5E-39 |
| 20 | g8185.t10 | PIRSF | PIRSF002419 | Tetraspanin | 1 | 218 | 8.7E-43 |
| 6 | g8185.t10 | PRINTS | PR00259 | Transmembrane four family signature | 38 | 64 | 2.6E-28 |
| 4 | g8185.t10 | PRINTS | PR00259 | Transmembrane four family signature | 65 | 93 | 2.6E-28 |
| 5 | g8185.t10 | PRINTS | PR00259 | Transmembrane four family signature | 183 | 209 | 2.6E-28 |
| 1 | g8185.t10 | Pfam | PF00335 | Tetraspanin family | 3 | 207 | 1.5E-41 |
| 13 | g8185.t10 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 14 | g8185.t10 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 15 | g8185.t10 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 15 | - |
| 19 | g8185.t10 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 19 | - |
| 11 | g8185.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 42 | - |
| 17 | g8185.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 43 | 64 | - |
| 9 | g8185.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 65 | 75 | - |
| 18 | g8185.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 76 | 95 | - |
| 12 | g8185.t10 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 96 | 182 | - |
| 16 | g8185.t10 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 183 | 206 | - |
| 10 | g8185.t10 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 207 | 218 | - |
| 26 | g8185.t10 | ProSitePatterns | PS00421 | Transmembrane 4 family signature. | 49 | 71 | - |
| 7 | g8185.t10 | SUPERFAMILY | SSF48652 | Tetraspanin | 93 | 179 | 1.83E-15 |
| 25 | g8185.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 2 | 24 | - |
| 23 | g8185.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 39 | 61 | - |
| 24 | g8185.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 74 | 96 | - |
| 22 | g8185.t10 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 183 | 205 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016021 | integral component of membrane | CC |
KEGG
Orthology
- This transcript has been annotated with K06497 (CD63 antigen)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
