General information for g8185.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as hypothetical.
Parent gene
- The parent gene g8185 is located on chr_2: 28963057..28964797 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8185 | g8185.t4 | isoform | g8185.t4 | 28961709 | 28962581 |
| chr_2 | g8185 | g8185.t4 | exon | g8185.t4.exon1 | 28961709 | 28962254 |
| chr_2 | g8185 | g8185.t4 | TTS | g8185.t4 | 28961757 | 28961757 |
| chr_2 | g8185 | g8185.t4 | cds | g8185.t4.CDS1 | 28962204 | 28962254 |
| chr_2 | g8185 | g8185.t4 | exon | g8185.t4.exon2 | 28962328 | 28962581 |
| chr_2 | g8185 | g8185.t4 | cds | g8185.t4.CDS2 | 28962328 | 28962579 |
| chr_2 | g8185 | g8185.t4 | TSS | g8185.t4 | NA | NA |
Sequences
>g8185.t4 Gene=g8185 Length=800
GAGACACAAATAATGAAACAAGAATTGCTATCGAAGGCATACAACGCGGACTTCGCTGTT
GTGGTTCAGCTGGACCCGCTGATTGGGGATTATTAAATATTCCATCTTCATGCTGTGATG
ATGGAGTTATAGCATGCACAGTGTCGAACGCTCATTCAGATGGATGTGCTACACTTCTTT
CCAACTTTGTCAATGATAGTGGACTCTTGATTGCATGGTTTGCAATTGTCTTTGCTGGTA
TTGAACTTGTTGGTATTTTAGTGGCCAATTCAATTCGAAATGCAAACAGAAGACAATATG
CATAAAAGTCTACAATGTACTAATTTAATAGAAATAGGAATTTGAAACCAAATTTTTTTT
GTTTAAAAATCTATTCTTATATATCTCTAATTAATTTTATAGTACAGCTATAGATTGATG
TCAAGAAAATAAGTTTTTTTTTCACTTATTTATCGCTATTAATTGATAAGTAAGTTGAAT
ACATATGTGTGTGTGTCACCATTTCTTTGGTCTATCATCTATATCAATTCTAATTTATCA
ATCTTAAGTTTCTGTGTGAGCTAGGCACGGCAGATAAGGGTCACTTCTATATATGCAAGA
GAAAAGAAAACACGCCAACAAGAAATATTTTATTTTTATTTTAGTCTTTTAAAATATGTT
ATCGTAAAAATTGTATATTTTTTATTCTTTAAATGTTGTACTTTGAACACAAGTTGTTAA
TAAACAAAGTAAAAGTGATTAGTTCTTGAATAAATGCTTTAAATATCAACAATTCAAAAT
TTAATGGTAAATAATAATTT
>g8185.t4 Gene=g8185 Length=100
DTNNETRIAIEGIQRGLRCCGSAGPADWGLLNIPSSCCDDGVIACTVSNAHSDGCATLLS
NFVNDSGLLIAWFAIVFAGIELVGILVANSIRNANRRQYA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g8185.t4 | CDD | cd03127 | tetraspanin_LEL | 2 | 65 | 5.47732E-14 |
| 4 | g8185.t4 | Gene3D | G3DSA:1.10.1450.10 | Tetraspanin | 1 | 64 | 4.4E-11 |
| 1 | g8185.t4 | Pfam | PF00335 | Tetraspanin family | 8 | 90 | 3.1E-12 |
| 6 | g8185.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 68 | - |
| 7 | g8185.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 69 | 88 | - |
| 5 | g8185.t4 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 89 | 100 | - |
| 3 | g8185.t4 | SUPERFAMILY | SSF48652 | Tetraspanin | 3 | 65 | 3.01E-12 |
| 2 | g8185.t4 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 69 | 91 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016021 | integral component of membrane | CC |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed