General information for g8214.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as V-type proton ATPase catalytic subunit A isoform 2.
Parent gene
- The parent gene g8214 is located on chr_2: 29091782..29092447 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8214 | g8214.t1 | TTS | g8214.t1 | 29090999 | 29090999 |
| chr_2 | g8214 | g8214.t1 | isoform | g8214.t1 | 29091782 | 29092447 |
| chr_2 | g8214 | g8214.t1 | exon | g8214.t1.exon1 | 29091782 | 29092447 |
| chr_2 | g8214 | g8214.t1 | cds | g8214.t1.CDS1 | 29091782 | 29092447 |
| chr_2 | g8214 | g8214.t1 | TSS | g8214.t1 | 29093433 | 29093433 |
Sequences
>g8214.t1 Gene=g8214 Length=666
ATGTTGCAAGTGTGGCCTGTTCGTCAGCCACGTCCAGTCACTGAAAAATTGCCAGCAAAT
CATCCCTTGCTGACTGGTCAGCGTGTCTTGGATTCATTGTTTCCATGTGTTCAAGGAGGA
ACAACTGCTATACCTGGTGCTTTCGGTTGTGGAAAAACTGTTATTTCGCAAGCCCTTTCA
AAATATTCCAATTCAGATGTCATTATTTATGTCGGTTGCGGAGAACGTGGTAATGAAATG
TCTGAAGTACTTCGTGATTTCCCAGAATTGTCTGTTGAAATTGATGGCGTAACTGAGTCT
ATCATGAAGCGTACAGCTCTTGTCGCTAACACATCAAATATGCCTGTCGCTGCTCGTGAA
GCTTCCATTTATACTGGTATTACACTTTCTGAATACTTCCGTGATATGGGTTATAATGTC
TCTATGATGGCTGATTCGACTTCTCGTTGGGCTGAGGCTCTTCGTGAAATTTCAGGTCGT
CTCGCTGAAATGCCTGCTGATTCAGGTTATCCAGCTTATTTGGGAGCTCGTCTTGCCTCA
TTTTACGAACGTGCTGGTCGTGTTAAATGTTTGGTAATCCAGAACGTGAAGGTTCAGTAT
CAATTGTCGGCGCTGTATCGCCACCTGGCGGTGACTTCTCAGATCCTGTTACATCTGCTA
CTTTAG
>g8214.t1 Gene=g8214 Length=221
MLQVWPVRQPRPVTEKLPANHPLLTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALS
KYSNSDVIIYVGCGERGNEMSEVLRDFPELSVEIDGVTESIMKRTALVANTSNMPVAARE
ASIYTGITLSEYFRDMGYNVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLAS
FYERAGRVKCLVIQNVKVQYQLSALYRHLAVTSQILLHLLL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g8214.t1 | CDD | cd01134 | V_A-ATPase_A | 1 | 191 | 0 |
| 5 | g8214.t1 | Gene3D | G3DSA:3.40.50.300 | - | 1 | 208 | 0 |
| 2 | g8214.t1 | PANTHER | PTHR43607:SF1 | V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A | 1 | 192 | 0 |
| 3 | g8214.t1 | PANTHER | PTHR43607 | V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A | 1 | 192 | 0 |
| 1 | g8214.t1 | Pfam | PF00006 | ATP synthase alpha/beta family, nucleotide-binding domain | 26 | 190 | 0 |
| 4 | g8214.t1 | SUPERFAMILY | SSF52540 | P-loop containing nucleoside triphosphate hydrolases | 2 | 190 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046034 | ATP metabolic process | BP |
| GO:0005524 | ATP binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
