General information for g8225.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein argonaute-2.
Parent gene
- The parent gene g8225 is located on chr_2: 29124055..29129946 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8225 | g8225.t1 | TTS | g8225.t1 | 29123383 | 29123383 |
| chr_2 | g8225 | g8225.t1 | isoform | g8225.t1 | 29124055 | 29129946 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon1 | 29124055 | 29124193 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS1 | 29124055 | 29124193 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon2 | 29124255 | 29124424 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS2 | 29124255 | 29124424 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon3 | 29124485 | 29124628 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS3 | 29124485 | 29124628 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon4 | 29124696 | 29124814 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS4 | 29124696 | 29124814 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon5 | 29124882 | 29125109 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS5 | 29124882 | 29125109 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon6 | 29125172 | 29125373 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS6 | 29125172 | 29125373 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon7 | 29125434 | 29125748 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS7 | 29125434 | 29125748 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon8 | 29126069 | 29126234 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS8 | 29126069 | 29126234 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon9 | 29126299 | 29127460 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS9 | 29126299 | 29127460 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon10 | 29128392 | 29128466 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS10 | 29128392 | 29128466 |
| chr_2 | g8225 | g8225.t1 | exon | g8225.t1.exon11 | 29129928 | 29129946 |
| chr_2 | g8225 | g8225.t1 | cds | g8225.t1.CDS11 | 29129928 | 29129946 |
| chr_2 | g8225 | g8225.t1 | TSS | g8225.t1 | 29130145 | 29130145 |
Sequences
>g8225.t1 Gene=g8225 Length=2739
ATGTTTCCAGTTGGACCTCAATCATCATGGACTTCTTCGCCTCCGCGGCCACCAAGTCCT
TCTCAGTCGCAGACCAGCTTCGATACACTTTCACCTCCTCCAGCTGGCGCTACGAATCCA
ACTACAACAACAGCTGGTCAAAGTGTTACTCCGAGTGTTGGAGTTGTTGCGACACCTCCA
GCACCGCCAGATTTGCCCGTTTTCACATGTCCGCGACGACCAAATCTTGGCCGAGAGGGC
CGTCCAATTTTATTGCGTGCAAATCACTTCCAAATCTCAATGCCACGCGGATTCGTTCAT
CACTATGACATCAACATACAACCAGATAAATGTCCACGCAAAGTGAATCGCGAAATTATT
GAAACAATGGTTCATGCTTATAGCAAAATTTTTGGTGTATTAAAACCAGTATTTGATGGA
CGTAACAATTTATATACACGTGATCCATTGCCAATTGGAAATGATAGAGTCGAATTGGAA
GTTACATTGCCTGGTGAAGGCAAGGATCGTGTTTTTCGTGTCACTATTAAATGGGTAGCA
CAAGTTTCATTGTTTAATCTCGAAGAGGCTCTTGAGGGTCGTACAAGACAAATTCCTTTT
GATGCAATTCAAGCACTCGACGTTGTTATGCGTCATTTACCAAGTATGACATACACACCT
GTTGGTCGCAGTTTCTTCAGTTCTCCCGATGGTTATTATCATCCGCTTGGTGGTGGACGT
GAAGTATGGTTCGGTTTTCATCAAAGCGTTCGTCCTTCTCAATGGAAAATGATGCTTAAT
ATTGATGTCTCAGCTACAGCATTCTACAAAGCTCAACCAGTCATTGAATTCATGTGTGAA
GTACTCGACATTCGTGACATTAATGAACAGAGAAAACCTCTTACTGACTCGCAACGTGTA
AAATTCACGAAGGAAATTAAAGGCTTGAAAATTGAAATAACACATTGTGGCACAATGAGA
CGTAAATATCGTGTCTGTAATGTTACAAGACGCCCTGCTCAAATGCAAAGCTTCCCACTT
CAACTCGATAATGGTCAAACTGTCGAGTGCACTGTTGCTAAATATTTCTTGGATAAATAC
AAGATGAAATTACGCTATCCACATTTACCATGCTTACAAGTCGGACAAGAGCATAAACAT
ACTTACTTGCCATTGGAAGTTTGTAATATTGTAGCTGGACAACGCTGTATCAAAAAACTT
ACAGATATGCAGACTTCAACAATGATCAAAGCTACTGCTAGATCTGCTCCTGATCGTGAA
CGTGAAATTAACAATTTGGTACGACGTGCTGACTTTAATAATGATGCATATGTTCAAGAG
TTTGGATTGACAATTAGCAACAACATGATGGAGGTTCGTGGTCGAGTCTTGCCACCACCA
AAACTTCAATATGGCGGTCGCGTTTCAAGCATTAGTGGACAAAATAAAGTGAGCTTGGCA
TCGCCTAATCAAGGCGTTTGGGATATGCGAGGAAAGCAGTTCTTTACAGGTGTTGAAATT
CGTGTATGGGCTATTGCTTGTTTTGCACCACAACGAACAGTTCGTGAAGATGCATTAAGA
AACTTTACACAGCAATTGCAAAAAATTTCAAATGATGCCGGAATGCCAATTATTGGACAG
CCATGCTTCTGCAAATATGCCACTGGACCTGATCAGGTTGAACCCATGTTTCGTTACTTG
AAAAACTCTTTCAATCATTTGCAACTTGTCGTTGTTGTATTGCCTGGAAAAACACCTGTG
TATGCAGAAGTAAAGAGAGTTGGTGATACAGTTTTAGGAATGGCAACACAATGCGTACAA
GCAAAGAACGTAAATAAAACATCGCCACAGACACTTTCGAATTTGTGTTTGAAAATCAAT
GTTAAATTGGGAGGAATCAATTCCATTTTGGTTCCATCTATTAGACCCAAAGTATTTAAT
GAACCAGTCATTTTCTTAGGTGCTGATGTAACTCATCCACCAGCTGGTGATAACAAAAAA
CCTTCAATTGCTGCTGTTGTTGGCTCAATGGATGCTCACCCTTCACGATATGCTGCTACA
GTACGTGTTCAACAGCATCGACAAGAAATGATTCAAGAGTTAAGCAGTATGGTACGTGAA
TTGCTAGTTATGTTCTATAAATCAACAGGTGGCTACAAACCACATCGTATTATTTTGTAT
CGAGATGGCGTCTCTGAGGGTCAATTTCCACATATATTACAACATGAACTTACAGCTATT
CGTGAAGCTTGCATTAAATTAGAAGCAGATTATCGTCCGGGTATCACATTTATAGTTGTT
CAAAAAAGACATCATACACGCTTGTTCTGTGCTGATAAGAAAGAACAAAGTGGAAAGAGT
GGAAATATTCCAGCAGGAACAACTGTCGACGTCGGTATTACTCATCCTACAGAATTCGAT
TTCTACTTATGCTCACATCAGGGTATTCAAGGAACAAGTCGACCATCACATTATCATGTA
TTATGGGATGATAATCACTTTGATTCAGATGAACTACAATGTTTAACTTATCAATTATGT
CATACTTATGTACGATGTACGCGATCAGTTAGTATTCCTGCACCTGCTTATTACGCTCAT
CTTGTTGCATTCAGAGCAAGATATCATTTAGTTGAAAAAGAACATGATTCAGGAGAAGGT
TCCCATCAAAGTGGATGTTCTGAAGACCGTACACCAGGCGCAATGGCACGAGCTATAACA
GTGCATGCAGATACCAAAAAAGTTATGTACTTTGCTTAA
>g8225.t1 Gene=g8225 Length=912
MFPVGPQSSWTSSPPRPPSPSQSQTSFDTLSPPPAGATNPTTTTAGQSVTPSVGVVATPP
APPDLPVFTCPRRPNLGREGRPILLRANHFQISMPRGFVHHYDINIQPDKCPRKVNREII
ETMVHAYSKIFGVLKPVFDGRNNLYTRDPLPIGNDRVELEVTLPGEGKDRVFRVTIKWVA
QVSLFNLEEALEGRTRQIPFDAIQALDVVMRHLPSMTYTPVGRSFFSSPDGYYHPLGGGR
EVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRDINEQRKPLTDSQRV
KFTKEIKGLKIEITHCGTMRRKYRVCNVTRRPAQMQSFPLQLDNGQTVECTVAKYFLDKY
KMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDRE
REINNLVRRADFNNDAYVQEFGLTISNNMMEVRGRVLPPPKLQYGGRVSSISGQNKVSLA
SPNQGVWDMRGKQFFTGVEIRVWAIACFAPQRTVREDALRNFTQQLQKISNDAGMPIIGQ
PCFCKYATGPDQVEPMFRYLKNSFNHLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQCVQ
AKNVNKTSPQTLSNLCLKINVKLGGINSILVPSIRPKVFNEPVIFLGADVTHPPAGDNKK
PSIAAVVGSMDAHPSRYAATVRVQQHRQEMIQELSSMVRELLVMFYKSTGGYKPHRIILY
RDGVSEGQFPHILQHELTAIREACIKLEADYRPGITFIVVQKRHHTRLFCADKKEQSGKS
GNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDSDELQCLTYQLC
HTYVRCTRSVSIPAPAYYAHLVAFRARYHLVEKEHDSGEGSHQSGCSEDRTPGAMARAIT
VHADTKKVMYFA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g8225.t1 | CDD | cd02846 | PAZ_argonaute_like | 271 | 391 | 1.18604E-42 |
| 14 | g8225.t1 | CDD | cd04657 | Piwi_ago-like | 437 | 870 | 0.0 |
| 11 | g8225.t1 | Gene3D | G3DSA:2.170.260.10 | paz domain | 265 | 397 | 2.7E-48 |
| 13 | g8225.t1 | Gene3D | G3DSA:3.40.50.2300 | - | 456 | 633 | 1.6E-63 |
| 12 | g8225.t1 | Gene3D | G3DSA:3.30.420.10 | - | 634 | 912 | 1.0E-135 |
| 19 | g8225.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 50 | - |
| 20 | g8225.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 22 | 50 | - |
| 7 | g8225.t1 | PANTHER | PTHR22891 | EUKARYOTIC TRANSLATION INITIATION FACTOR 2C | 52 | 911 | 0.0 |
| 8 | g8225.t1 | PANTHER | PTHR22891:SF59 | PROTEIN ARGONAUTE-2 | 52 | 911 | 0.0 |
| 6 | g8225.t1 | Pfam | PF16486 | N-terminal domain of argonaute | 81 | 210 | 2.1E-28 |
| 5 | g8225.t1 | Pfam | PF08699 | Argonaute linker 1 domain | 220 | 270 | 6.9E-23 |
| 3 | g8225.t1 | Pfam | PF02170 | PAZ domain | 290 | 409 | 1.1E-24 |
| 4 | g8225.t1 | Pfam | PF16488 | Argonaute linker 2 domain | 418 | 464 | 3.6E-16 |
| 1 | g8225.t1 | Pfam | PF16487 | Mid domain of argonaute | 481 | 562 | 9.4E-33 |
| 2 | g8225.t1 | Pfam | PF02171 | Piwi domain | 570 | 870 | 4.3E-115 |
| 22 | g8225.t1 | ProSiteProfiles | PS50821 | PAZ domain profile. | 272 | 392 | 30.795 |
| 21 | g8225.t1 | ProSiteProfiles | PS50822 | Piwi domain profile. | 569 | 871 | 50.149 |
| 16 | g8225.t1 | SMART | SM01163 | DUF1785_2 | 219 | 271 | 8.4E-28 |
| 17 | g8225.t1 | SMART | SM00949 | PAZ_2_a_3 | 279 | 414 | 7.0E-5 |
| 18 | g8225.t1 | SMART | SM00950 | Piwi_a_2 | 569 | 871 | 1.3E-135 |
| 10 | g8225.t1 | SUPERFAMILY | SSF101690 | PAZ domain | 83 | 433 | 3.7E-63 |
| 9 | g8225.t1 | SUPERFAMILY | SSF53098 | Ribonuclease H-like | 565 | 872 | 1.19E-90 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
| GO:0003676 | nucleic acid binding | MF |
KEGG
Orthology
- This transcript has been annotated with K11593 (eukaryotic translation initiation factor 2C)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
