General information for g823.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial.
Parent gene
- The parent gene g823 is located on chr_3: 6266468..6270027 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g823 | g823.t1 | TTS | g823.t1 | 6263611 | 6263611 |
| chr_3 | g823 | g823.t1 | isoform | g823.t1 | 6263835 | 6268419 |
| chr_3 | g823 | g823.t1 | exon | g823.t1.exon1 | 6263835 | 6263949 |
| chr_3 | g823 | g823.t1 | cds | g823.t1.CDS1 | 6263835 | 6263949 |
| chr_3 | g823 | g823.t1 | exon | g823.t1.exon2 | 6264350 | 6265255 |
| chr_3 | g823 | g823.t1 | cds | g823.t1.CDS2 | 6264350 | 6265255 |
| chr_3 | g823 | g823.t1 | exon | g823.t1.exon3 | 6265420 | 6265474 |
| chr_3 | g823 | g823.t1 | cds | g823.t1.CDS3 | 6265420 | 6265474 |
| chr_3 | g823 | g823.t1 | exon | g823.t1.exon4 | 6266159 | 6266192 |
| chr_3 | g823 | g823.t1 | cds | g823.t1.CDS4 | 6266159 | 6266192 |
| chr_3 | g823 | g823.t1 | exon | g823.t1.exon5 | 6266299 | 6266366 |
| chr_3 | g823 | g823.t1 | cds | g823.t1.CDS5 | 6266299 | 6266366 |
| chr_3 | g823 | g823.t1 | exon | g823.t1.exon6 | 6266441 | 6266801 |
| chr_3 | g823 | g823.t1 | cds | g823.t1.CDS6 | 6266441 | 6266801 |
| chr_3 | g823 | g823.t1 | exon | g823.t1.exon7 | 6268390 | 6268419 |
| chr_3 | g823 | g823.t1 | cds | g823.t1.CDS7 | 6268390 | 6268419 |
| chr_3 | g823 | g823.t1 | TSS | g823.t1 | NA | NA |
Sequences
>g823.t1 Gene=g823 Length=1569
ATGTCGCTCTTCAGAATCGCATCAGCGGAGGTACGCAATGTTATCAAGCGCTCATATAAC
ACCACAAATGTCGCACCCACTGTCAAAATGTTCATTGATGGAAAATTTGTTGATTCACAA
GCCAAAGAATGGATTGATTTACATGATCCAGCAACAAATGAGCTTGTAACACGCGTTCCA
AAGTGCACTCTTGATGAAATGAATGCTGCTGTTGAATCATCGAAACGCGCTTTTAAGACA
TGGAGTCAAACATCAATTCTTACACGTCAGCAAGTCATGCTCAAATTGCAGCATATTATT
CGCGCCAATATGTCTGAATTAGCTAAGAATATTACAAAAGAACAGGGAAAAACACTTGTC
GATGCTGAAGGCGATGTTTTAAGAGGATTGCAGGTTGTTGAGCACATGTGCAGCATTACA
TCGCTTCAACAAGGCGAGACGATGCCAAATATTGCAAAGGATATGGATACTTATAGTTAT
ACTCTTCCACTTGGAGTTACTGCTGGAATTACACCTTTCAATTTTCCTGCTATGATTCCA
CTTTGGATGTTCCCTGTTGCAATTACATGTGGAAATACGAGCATCATTAAGCCATCTGAA
AGAGATCCAGGTGCAACACTTTTACTCTGTGAAATGCTCAATGAAGCAGGCTGTCCTCCA
GGCGTTGTAAACGTTATTCATGGCGCTCACGATGCGGTCAATTTCATCTGTGATCATCCC
GATATCAAGGCAATTTCATTCGTTGGTTCTGACCAGGCTGGCAAATACATTTACGAACGT
GGTTGCCGCAATGGTAAACGTGTACAAAGCAACATGGGTGCAAAGAATCATGGTGTGGTA
ATGGCTGACGCAAATAAGAATGCAACATTAAATCAACTCGCTGGTGCTGCATTTGGTGCT
GCTGGTCAACGTTGTATGGCATTGTCAACTGCAATCTTTGTTGGTGAAGCACGTGAATGG
ATTCCTGATTTGGTTGAACGAGCTGCAAAATTAAAAGTTGGTCCAGGAGACAAACCCGGT
GTTGATCTCGGTCCAGTCATTTCACCACAATCAAAGAAACGTATTCTCGATCTTGTGCAG
TCAGGCGTCGATGAAGGCGCGAAAATCGTCCTTGATGGACGTGACATTAAAATTGATGGC
TATGAAAAGGGTAACTTTGTTGGACCAACAATTCTCGTCAATGTGACGCCTTCAATGAAA
TGCTATAAAGAAGAAATTTTCGGACCAGTTCTTGTATGTTTAACGGCAGAGACGCTTGAT
GAAGCCATTGACATCATTAATAAGAACCCATACGGCAATGGCACAGCTATTTTCACAACG
AATGGCGCGACAGCGAGGAAATTTGTCAATGAAATTGATGTTGGACAAGTTGGCGTTAAT
GTTCCCATTCCTGTGCCATTACCAATGTTCTCATTCACTGGATCACGTGGTAGTTTCTGG
GGCTCAGAACATTTCTACGGTAAGCAAGGCATCAAGTTCTATACCCAAACGAAGACGGTT
ACTCAATTGTGGCGCGAGACAGATGTGACGCATACTCAAGCAGCAGTTGCCATGCCCACA
ATGAAATAA
>g823.t1 Gene=g823 Length=522
MSLFRIASAEVRNVIKRSYNTTNVAPTVKMFIDGKFVDSQAKEWIDLHDPATNELVTRVP
KCTLDEMNAAVESSKRAFKTWSQTSILTRQQVMLKLQHIIRANMSELAKNITKEQGKTLV
DAEGDVLRGLQVVEHMCSITSLQQGETMPNIAKDMDTYSYTLPLGVTAGITPFNFPAMIP
LWMFPVAITCGNTSIIKPSERDPGATLLLCEMLNEAGCPPGVVNVIHGAHDAVNFICDHP
DIKAISFVGSDQAGKYIYERGCRNGKRVQSNMGAKNHGVVMADANKNATLNQLAGAAFGA
AGQRCMALSTAIFVGEAREWIPDLVERAAKLKVGPGDKPGVDLGPVISPQSKKRILDLVQ
SGVDEGAKIVLDGRDIKIDGYEKGNFVGPTILVNVTPSMKCYKEEIFGPVLVCLTAETLD
EAIDIINKNPYGNGTAIFTTNGATARKFVNEIDVGQVGVNVPIPVPLPMFSFTGSRGSFW
GSEHFYGKQGIKFYTQTKTVTQLWRETDVTHTQAAVAMPTMK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g823.t1 | CDD | cd07085 | ALDH_F6_MMSDH | 28 | 504 | 0.0 |
| 6 | g823.t1 | Gene3D | G3DSA:3.40.605.10 | Aldehyde Dehydrogenase; Chain A | 18 | 273 | 1.8E-77 |
| 7 | g823.t1 | Gene3D | G3DSA:3.40.309.10 | Aldehyde Dehydrogenase; Chain A | 275 | 507 | 4.7E-64 |
| 2 | g823.t1 | PANTHER | PTHR43866:SF3 | METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE [ACYLATING], MITOCHONDRIAL | 9 | 521 | 1.4E-276 |
| 3 | g823.t1 | PANTHER | PTHR43866 | MALONATE-SEMIALDEHYDE DEHYDROGENASE | 9 | 521 | 1.4E-276 |
| 1 | g823.t1 | Pfam | PF00171 | Aldehyde dehydrogenase family | 36 | 500 | 6.2E-137 |
| 5 | g823.t1 | ProSitePatterns | PS00070 | Aldehyde dehydrogenases cysteine active site. | 298 | 309 | - |
| 4 | g823.t1 | SUPERFAMILY | SSF53720 | ALDH-like | 18 | 502 | 3.53E-143 |
| 8 | g823.t1 | TIGRFAM | TIGR01722 | MMSDH: methylmalonate-semialdehyde dehydrogenase (acylating) | 28 | 504 | 3.0E-239 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016620 | oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor | MF |
| GO:0004491 | methylmalonate-semialdehyde dehydrogenase (acylating) activity | MF |
| GO:0055114 | NA | NA |
| GO:0016491 | oxidoreductase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00140 (malonate-semialdehyde dehydrogenase (acetylating) / methylmalonate-semialdehyde dehydrogenase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
