General information for g8285.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Serine/threonine-protein kinase tricorner.
Parent gene
- The parent gene g8285 is located on chr_2: 29554806..29555788 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8285 | g8285.t1 | isoform | g8285.t1 | 29553169 | 29554806 |
| chr_2 | g8285 | g8285.t1 | exon | g8285.t1.exon1 | 29553169 | 29553664 |
| chr_2 | g8285 | g8285.t1 | cds | g8285.t1.CDS1 | 29553169 | 29553664 |
| chr_2 | g8285 | g8285.t1 | exon | g8285.t1.exon2 | 29553742 | 29554125 |
| chr_2 | g8285 | g8285.t1 | cds | g8285.t1.CDS2 | 29553742 | 29554125 |
| chr_2 | g8285 | g8285.t1 | exon | g8285.t1.exon3 | 29554187 | 29554288 |
| chr_2 | g8285 | g8285.t1 | cds | g8285.t1.CDS3 | 29554187 | 29554288 |
| chr_2 | g8285 | g8285.t1 | exon | g8285.t1.exon4 | 29554346 | 29554633 |
| chr_2 | g8285 | g8285.t1 | cds | g8285.t1.CDS4 | 29554346 | 29554633 |
| chr_2 | g8285 | g8285.t1 | exon | g8285.t1.exon5 | 29554694 | 29554806 |
| chr_2 | g8285 | g8285.t1 | cds | g8285.t1.CDS5 | 29554694 | 29554806 |
| chr_2 | g8285 | g8285.t1 | TTS | g8285.t1 | 29555125 | 29555125 |
| chr_2 | g8285 | g8285.t1 | TSS | g8285.t1 | NA | NA |
Sequences
>g8285.t1 Gene=g8285 Length=1383
ATGAATCCCACAATAGATAATTCAATTCGTTTTTCAAATCATACTATGGATAAGGCGACA
AAGGCTAAAGTTACATTAGAAAACTATTATAGTAATCTCATAGCTCAACATGGTGAGCGA
AAGCAGCGATTAGCTAAACTAGAGGCTTCTCTTAAAGATGAAGCATTAACAGAATCACAA
CGCCAAGAGAAGAGAGCTCAGCATGCTCAAAAGGAAACGGAATTTTTACGACTCAAAAGA
TCGCGTCTGGGTGTTGAAGACTTTGAAGCACTCAAAGTTATAGGTCGTGGTGCTTTTGGA
GAAGTTCGTTTGGTTCAAAAGAAAGATACTGGACATGTTTATGCAATGAAAGTTCTTCGA
AAGGCTGATATGTTAGAGAAGGAACAAGTTGCTCATGTGCGGGCAGAACGTGATGTTCTT
GTCGAAGCTGATCATCAATGGGTAGTAAAAATGTACTATAGTTTTCAAGATCATGTAAAT
TTATATTTAATTATGGAATTTTTACCTGGAGGTGATATGATGACGCTGTTAATGAAAAAG
GACACACTCACTGAAGAGTGTACGCAATTTTATATAGCTGAAACGGCATTAGCAATTGAC
TCTATACACAAACTAGGATTTATTCATCGAGACATAAAGCCTGACAATTTATTGCTTGAT
GCAAGAGGTCATTTAAAGCTATCGGATTTTGGTTTGTGTACAGGTCTTAAAAAGTCTCAC
CGTACAGATTTTTATCGTGATTTATCGCAAGCTAAACCATCAGATTTCATAGGAACATGT
GCAAGTCCTATGGACTCAAAGAGGCGAGCTGAAAGTTGGAAAAGAAATAGACGTGCACTT
GCCTACAGCACTGTTGGAACACCAGATTATATAAGTCCTGAGGTATTTTTACAAACCGGA
TATGGAGCAGCCTGTGACTACTGGTCTTTAGGTGTTATAATGTATGAAATGCTTATGGGA
TTTCCTCCATTTTGCTCTGATAATCCACAAGATACATATCGCAAAGTTATGAATTGGAGA
GAAACATTAGTTTTTCCACCTGAAACACCAATAACTGAAGAAGCGCGTGATACAATAGTA
AGATTTTGCTGTGAAGCTGAACGTCGTTTAGGTTCACAAAAAGGAATCGAAGAGCTGAAA
AATGTACCATTTTTCAAAGGTGTCGATTGGGAACATATCCGAGAGCGGCCTGCAGCTATT
CCCGTAGATGTTCGATCAATTGATGACACATCAAACTTTGACGATTTTCCTGATGTTGCT
TTAGAAATTCCTTCTGCACCTTGTCCGCAAGATGGTGAAGTTTTAAAAGATTGGGTCTTT
ATCAACTATACATTTAAGCGTTTTGAAGGATTAACACAAAGAGGTACACCGACTCACAAA
TAG
>g8285.t1 Gene=g8285 Length=460
MNPTIDNSIRFSNHTMDKATKAKVTLENYYSNLIAQHGERKQRLAKLEASLKDEALTESQ
RQEKRAQHAQKETEFLRLKRSRLGVEDFEALKVIGRGAFGEVRLVQKKDTGHVYAMKVLR
KADMLEKEQVAHVRAERDVLVEADHQWVVKMYYSFQDHVNLYLIMEFLPGGDMMTLLMKK
DTLTEECTQFYIAETALAIDSIHKLGFIHRDIKPDNLLLDARGHLKLSDFGLCTGLKKSH
RTDFYRDLSQAKPSDFIGTCASPMDSKRRAESWKRNRRALAYSTVGTPDYISPEVFLQTG
YGAACDYWSLGVIMYEMLMGFPPFCSDNPQDTYRKVMNWRETLVFPPETPITEEARDTIV
RFCCEAERRLGSQKGIEELKNVPFFKGVDWEHIRERPAAIPVDVRSIDDTSNFDDFPDVA
LEIPSAPCPQDGEVLKDWVFINYTFKRFEGLTQRGTPTHK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g8285.t1 | CDD | cd05599 | STKc_NDR_like | 86 | 446 | 0.0 |
| 7 | g8285.t1 | Coils | Coil | Coil | 52 | 72 | - |
| 5 | g8285.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 86 | 415 | 5.6E-103 |
| 6 | g8285.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 172 | 403 | 5.6E-103 |
| 2 | g8285.t1 | PANTHER | PTHR24356 | SERINE/THREONINE-PROTEIN KINASE | 14 | 456 | 2.3E-205 |
| 3 | g8285.t1 | PANTHER | PTHR24356:SF160 | SERINE/THREONINE-PROTEIN KINASE 38-LIKE | 14 | 456 | 2.3E-205 |
| 1 | g8285.t1 | Pfam | PF00069 | Protein kinase domain | 88 | 384 | 3.3E-58 |
| 10 | g8285.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 94 | 117 | - |
| 9 | g8285.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 207 | 219 | - |
| 13 | g8285.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 88 | 385 | 44.668 |
| 12 | g8285.t1 | ProSiteProfiles | PS51285 | AGC-kinase C-terminal domain profile. | 386 | 455 | 9.33 |
| 11 | g8285.t1 | SMART | SM00220 | serkin_6 | 88 | 385 | 2.9E-95 |
| 4 | g8285.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 75 | 405 | 7.28E-82 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004674 | protein serine/threonine kinase activity | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K08790 (serine/threonine kinase 38)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
