General information for g8288.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Kynurenine 3-monooxygenase.
Parent gene
- The parent gene g8288 is located on chr_2: 29563125..29564006 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8288 | g8288.t3 | isoform | g8288.t3 | 29563125 | 29564006 |
| chr_2 | g8288 | g8288.t3 | exon | g8288.t3.exon1 | 29563125 | 29563126 |
| chr_2 | g8288 | g8288.t3 | exon | g8288.t3.exon2 | 29563185 | 29563692 |
| chr_2 | g8288 | g8288.t3 | cds | g8288.t3.CDS1 | 29563191 | 29563692 |
| chr_2 | g8288 | g8288.t3 | exon | g8288.t3.exon3 | 29563756 | 29564006 |
| chr_2 | g8288 | g8288.t3 | cds | g8288.t3.CDS2 | 29563756 | 29564006 |
| chr_2 | g8288 | g8288.t3 | TTS | g8288.t3 | 29564091 | 29564091 |
| chr_2 | g8288 | g8288.t3 | TSS | g8288.t3 | NA | NA |
Sequences
>g8288.t3 Gene=g8288 Length=761
GATTTCAAATGCCTGAAAATTATCTTCATATATGGCCAAGAGGAAAATTCATGATGATTG
CATTGCCCAATCAAGATCGAACTTGGACTGTAACATTGTTCATGCCATTTGAAAATTTTG
ATAGTATCAAATCTTCAGAAGAGCTTCTAAAGTTTTTTGAAATTTATTTTCCTGATGCAA
TTCCATTAATTGGCAAAGAGCGTTTGGTCAAAGATTTTTTCAAAATTCCAGCTCAACATC
TCATAGCAGTAAAATGTCGTCCATATCATATATCATCAAAATGTGTTCTAATTGGAGATG
CAGGGCATGCTGTTGTTCCATTTTACGGCCAAGGAATGAATGCTGGCTTTGAAGACTGCA
GTATACTTACAGAGTTATTTAACAAATACGGCTCTGACTTAGAAAAAATTCTCACTGAAT
TTAGCGAGACAAGATGGATCGATGCACACGCTGTTTGTGATCTAGCGATGTACAATTACA
TTGAAATGAGAGATTTAGTCAACAAGAAATCATTTCTTATGAGAAAAGCATTAGATGATT
TTTTGCATTGGATTGCACCGTCATATTGGGTTCCTCTTTATAGCAGTGTTTCATTTACAT
GTATGCCATATAAGAAATGCATTGAAAATCGTGAATGGCAGGATCGCGTTTTAAATAAAA
TTTTTACTGTCTGTAGTTTTATCGGAGTAAGTTCATGCTTGTTAGGTGCTATTCACCTTT
ATAACACATATAACACTATGGAAGAATTGTCATTTAATTAA
>g8288.t3 Gene=g8288 Length=250
MPENYLHIWPRGKFMMIALPNQDRTWTVTLFMPFENFDSIKSSEELLKFFEIYFPDAIPL
IGKERLVKDFFKIPAQHLIAVKCRPYHISSKCVLIGDAGHAVVPFYGQGMNAGFEDCSIL
TELFNKYGSDLEKILTEFSETRWIDAHAVCDLAMYNYIEMRDLVNKKSFLMRKALDDFLH
WIAPSYWVPLYSSVSFTCMPYKKCIENREWQDRVLNKIFTVCSFIGVSSCLLGAIHLYNT
YNTMEELSFN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g8288.t3 | Gene3D | G3DSA:3.50.50.60 | - | 1 | 177 | 1.9E-67 |
| 2 | g8288.t3 | PANTHER | PTHR46028:SF2 | KYNURENINE 3-MONOOXYGENASE | 1 | 240 | 7.0E-90 |
| 3 | g8288.t3 | PANTHER | PTHR46028 | KYNURENINE 3-MONOOXYGENASE | 1 | 240 | 7.0E-90 |
| 1 | g8288.t3 | Pfam | PF01494 | FAD binding domain | 59 | 122 | 1.5E-5 |
| 8 | g8288.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 217 | - |
| 10 | g8288.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 218 | 238 | - |
| 9 | g8288.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 239 | 250 | - |
| 6 | g8288.t3 | SUPERFAMILY | SSF51905 | FAD/NAD(P)-binding domain | 80 | 187 | 6.99E-16 |
| 4 | g8288.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 178 | 197 | - |
| 5 | g8288.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 218 | 240 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0071949 | FAD binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed