General information for g8294.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as hypothetical.
Parent gene
- The parent gene g8294 is located on chr_2: 29595193..29596170 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8294 | g8294.t1 | TTS | g8294.t1 | 29594005 | 29594005 |
| chr_2 | g8294 | g8294.t1 | isoform | g8294.t1 | 29594986 | 29595906 |
| chr_2 | g8294 | g8294.t1 | exon | g8294.t1.exon1 | 29594986 | 29595125 |
| chr_2 | g8294 | g8294.t1 | cds | g8294.t1.CDS1 | 29594986 | 29595125 |
| chr_2 | g8294 | g8294.t1 | exon | g8294.t1.exon2 | 29595191 | 29595734 |
| chr_2 | g8294 | g8294.t1 | cds | g8294.t1.CDS2 | 29595191 | 29595734 |
| chr_2 | g8294 | g8294.t1 | exon | g8294.t1.exon3 | 29595805 | 29595906 |
| chr_2 | g8294 | g8294.t1 | cds | g8294.t1.CDS3 | 29595805 | 29595906 |
| chr_2 | g8294 | g8294.t1 | TSS | g8294.t1 | 29596173 | 29596173 |
Sequences
>g8294.t1 Gene=g8294 Length=786
ATGACTTCAACATTTTTTGATAAATTTCAATCGATTGTAATCGGAACAGGCAATGTTCTT
GATGATGAAGAGTTTAAAAAAGCTAAAACACCTACGCAAGAAATGCTTATTTGCCTTTCA
TCACGAATTAATGAAGTGAAAAAAACAAATGTGCAATCTGATTCTGATAAGTTTGTTTCA
ATTCCACATGAAAATGAGCAGAATGGAGCATCTGAAAATTCAGAAGGAAAAACTAAGACT
GTCTTGAAAATTTTCTTAAACGAATATCATCATGAAACGATTGAGGAAACATTGGGTTAT
ACTTTAAAACTTTGCAAAACAGCACCCAATGTAATCTTGTCTTACTCACCAACATCGAAA
ATTGAAAATGATAAATTTATTTGGGCAGAAAATGATGCTGCAAAAGCAAAATCAAATTTC
AAGTTGCTTTGGACAAAACTCCGAGAGCAAAAAAAGGCAAAAAAAATTGAACAATTGGGA
ATTGCTGATATGGATCTCGATACTATTCTCGATATTTTTGAAGATAAAAATTACGATTTC
ACGATCCTTCAGATCAATATTGCAACTTGCTGCATGCCACCACCTTGCTTGGTAAATTTT
TGTAAAGAAAATGAAATTCAGCTTTTAACACACTCTGATCCTCAAGTTTTGCTTCCATCT
TGTCATTTAGAAGAAGTCAGCCTAAGTGCATTTAAAGTGAAGTGGATTGTGCGGTATCTT
GAGAGTCTTGTTTGTAGAGGAATTCTCACAAAAAAGGGTTTTATTGTTAATTTTCAACGA
AAGTAA
>g8294.t1 Gene=g8294 Length=261
MTSTFFDKFQSIVIGTGNVLDDEEFKKAKTPTQEMLICLSSRINEVKKTNVQSDSDKFVS
IPHENEQNGASENSEGKTKTVLKIFLNEYHHETIEETLGYTLKLCKTAPNVILSYSPTSK
IENDKFIWAENDAAKAKSNFKLLWTKLREQKKAKKIEQLGIADMDLDTILDIFEDKNYDF
TILQINIATCCMPPPCLVNFCKENEIQLLTHSDPQVLLPSCHLEEVSLSAFKVKWIVRYL
ESLVCRGILTKKGFIVNFQRK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 3 | g8294.t1 | Gene3D | G3DSA:3.20.20.100 | - | 71 | 218 | 8.0e-07 |
| 1 | g8294.t1 | PANTHER | PTHR13295 | GLUTAMATE CYSTEINE LIGASE REGULATORY SUBUNIT | 2 | 260 | 0.0e+00 |
| 2 | g8294.t1 | SUPERFAMILY | SSF51430 | NAD(P)-linked oxidoreductase | 137 | 212 | 4.4e-06 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0035226 | glutamate-cysteine ligase catalytic subunit binding | MF |
| GO:0006750 | glutathione biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K11205 (glutamate–cysteine ligase regulatory subunit)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
