General information for g8316.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Diacylglycerol kinase 1.
Parent gene
- The parent gene g8316 is located on chr_2: 29676179..29683297 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8316 | g8316.t1 | isoform | g8316.t1 | 29673676 | 29683297 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon1 | 29673676 | 29673757 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS1 | 29673676 | 29673757 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon2 | 29673820 | 29673899 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS2 | 29673820 | 29673899 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon3 | 29673962 | 29674082 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS3 | 29673962 | 29674082 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon4 | 29677479 | 29677601 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS4 | 29677479 | 29677601 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon5 | 29677737 | 29677861 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS5 | 29677737 | 29677861 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon6 | 29678401 | 29678509 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS6 | 29678401 | 29678509 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon7 | 29679486 | 29679614 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS7 | 29679486 | 29679614 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon8 | 29679687 | 29680021 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS8 | 29679687 | 29680021 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon9 | 29680455 | 29680629 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS9 | 29680455 | 29680629 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon10 | 29680708 | 29680794 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS10 | 29680708 | 29680794 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon11 | 29680864 | 29680917 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS11 | 29680864 | 29680917 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon12 | 29680993 | 29681168 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS12 | 29680993 | 29681168 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon13 | 29681289 | 29681769 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS13 | 29681289 | 29681769 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon14 | 29681836 | 29682234 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS14 | 29681836 | 29682234 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon15 | 29682296 | 29682379 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS15 | 29682296 | 29682379 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon16 | 29682467 | 29682564 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS16 | 29682467 | 29682564 |
| chr_2 | g8316 | g8316.t1 | exon | g8316.t1.exon17 | 29683202 | 29683297 |
| chr_2 | g8316 | g8316.t1 | cds | g8316.t1.CDS17 | 29683202 | 29683297 |
| chr_2 | g8316 | g8316.t1 | TSS | g8316.t1 | NA | NA |
| chr_2 | g8316 | g8316.t1 | TTS | g8316.t1 | NA | NA |
Sequences
>g8316.t1 Gene=g8316 Length=2754
ATGGCATCGTGCAAACAATCTCAATCACAATGGGATAAGCTCTCACCAAACGAATTCCAG
CAGCTTCAAGACCTTGCAAGTTATTCCTCAAAAAAGCTAGAGGATGTGTTGGAAGAATTT
TGTGGCTCGACATCGGCAACATGCAAGTTCAGTCAGGATGATGACATTGACTTTGAGGGC
TTTCAAAAGTTTATTAACCTGTTTTTGGATTGTGAAACACCTTTGGACTTGTCACAGCAT
CTGTTTCTATCCTTCTTACGACCCTACCAATCGCAGTTATCAGATATGAAGTTATTGCCC
AGGAAGCAATCATTTTTGGATCCATTAGCCATGAAAGTTCCGCTCAAGGACGTCGTTTGC
TATCTTTCATTGCTTGAAGCAGGAAGACCCGAAGATAAATTGGAATTTATGTTCCGTCTT
TATGACAACGATGGCAATGGAGTATTGGATAAAATTGAAACTGATGCAATCGTTAATCAG
ATGATGTCAGTTGCTGAATATCTCGGTTGGGATGTTTCTGAATTGCGACCAATACTGCAA
GATATGATGACTGAGATAGACTATGATGGTGATGGTAGTGTGTCACTAGATGAATGGAAA
CGTGGTGGCTTGACGACAATTCCATTGTTAGTTCTATTAGGTGTTGATAGTTCACTTAAG
GAAGATGGCTATCACGTGTGGCGATTAAAACATTTCTCGAAGCCTGCTTATTGCAATCTT
TGCCTCAACATGTTGGTCGGACTAGGAAAAAAGGGATTGTGCTGTACACTATGCAAGTAT
ACAGTTCATGAACGTTGTGTTCAAAGAGCACCGGCTTCATGTATCACCACTTATGTGAAA
TCAAAACGACCTGGCGCTTTTCAGCATCATTGGGTCGAAGGAAATTGCTATCGGAATTGT
TCAAAGTGCCACAAACGCATTAAAGCTTATGTCGGTCTCACGTGTCGTTGGTGTCAAATG
ACCTTACACAATAGATGCGCGAGCAACGTGAAAATTGAATGTAATTTAGGAAAATTTTCT
AACTTTGTTCTTCCTCCCACTGCCATATGCCCAGCCGTATTAGATCGTCAGAGGTCGGCA
AGCGTAACCAACATCAAGTCAAAGCCAGCACCGCCAATTCATTTTCAAATTACACCACCC
TCAGCCTCATCGACGCCTCTTCTCGTGTTTATAAATCCAAAAAGTGGCGGACGCCAGGGT
GAGAGAATTTTGCGGAAATTTCAGTATTTGTTGAATCCAAGACAGGTATATGATCTGAGT
AAGGGTGGACCGCTTGAGGGACTCACAATGTTCAAAGACGTGCCGAATTTTATTGTCATT
TGTTGTGGTGGTGATGGAACTGTTGGATGGATATTAGAGGCTATGGATTCAGTTGAGATG
AAACAACGACCGCCAGTAGCTGTCATACCACTCGGAACAGGTAATGATCTGGCACGTTGT
TTAAGATGGGGTGGTGGATATGAAGGCGAAAGTATACCCAAACTGCTCGAGAAAATTAGC
CGCTCCGAAGCAGTGATGATGGATAGATGGAATGTTAGCATTGAAGCAACGGGTGATAAA
GAAGAAACCATAATTCCTTCACATAAAGTATTAAAGGTCACACTATCAGAAAATGTACAA
AGAGTAGTTGAATTGTCGCAAAGAATAGTTGTTGAAAAGTCTGTGATAAATCAAAATGAA
CAAGTGCAAAAAACTTCGGAAATATCAATTGAAAACTCTAAACGTGTCATTTTGAGTGAC
AATAATTCATCTTCCACGTCAACGACATTTGAAAAGAGTCGTGAAAAGACGCTTGAATCA
AACGGTATGTTGCAGAATAGTGAAAGGATTTGTGACAAAGAATGCACTGTTGAACGCAAA
TCGAAGGGTGGAGAAGAAAACTGTTGCAATGATGCTGAAAGAAATAACAATAATAATAAT
ATAAATGACAATGAAGCGATTAAGAAACCAAAAAGCAGCACGAGTTGTAATGATCTATTA
ATGTTTGGAAAGCAACATTCACATAGAGAAGAAATTGTGCCGACAACTGCTGCTGATTTT
TCTATACCGTATAATATTATCAATAATTATTTTTCAGTTGGAGTTGATGCAGCAATTTGT
GTAAAATTTCATTTAGAACGTGAAAAAAATCCACATAAATTCAATTCACGCATGAAAAAT
AAGTTATGGTATTTCGAATACGCTACAAGTGAAACATTCTCGGCAACATGTAAAAACTTG
CACGAATATGTTGAGCTGACATGTGATGGCATTCAATTAGACTTAAGTGGAATTCAACTT
CAAGGAATAGCTTTATTAAACATTCCCTACACACACGGTGGCTCTAATTTATGGGGTGAG
AATTTATCACAGAAGCGACTTAGAAGAAGTACATCAAGTAATGGACCATTTAAGAAAATT
AAGAAACTTCGCAGTGCTGATAAAGAAATGTCAACGCAAAGTTTCAATTCTGTTGACTTA
AGTATAGCAATACAAGATATTGGTGATAGAAAAATCGAAGTGATTGGACTAGAAAGTTGT
CTTCACATGGGTCAAGTTAGAACAGGATTGAGAGCATCAGGTCGACGATTGGCGCAGTGC
AGTGAAATTGTATTAAAGACTAAAAAGACTTTTCCTATGCAAATTGATGGTGAACCATGG
ATGCAAGAGCCTGCAACTATTAAGATAACACACAAGAATCAAGTTCCAATGTTGGCATCA
CCGAAGGCTGACAAAGGCAGAGGATTTTTCAATTTTAACTTCTTCAGAAGATGA
>g8316.t1 Gene=g8316 Length=917
MASCKQSQSQWDKLSPNEFQQLQDLASYSSKKLEDVLEEFCGSTSATCKFSQDDDIDFEG
FQKFINLFLDCETPLDLSQHLFLSFLRPYQSQLSDMKLLPRKQSFLDPLAMKVPLKDVVC
YLSLLEAGRPEDKLEFMFRLYDNDGNGVLDKIETDAIVNQMMSVAEYLGWDVSELRPILQ
DMMTEIDYDGDGSVSLDEWKRGGLTTIPLLVLLGVDSSLKEDGYHVWRLKHFSKPAYCNL
CLNMLVGLGKKGLCCTLCKYTVHERCVQRAPASCITTYVKSKRPGAFQHHWVEGNCYRNC
SKCHKRIKAYVGLTCRWCQMTLHNRCASNVKIECNLGKFSNFVLPPTAICPAVLDRQRSA
SVTNIKSKPAPPIHFQITPPSASSTPLLVFINPKSGGRQGERILRKFQYLLNPRQVYDLS
KGGPLEGLTMFKDVPNFIVICCGGDGTVGWILEAMDSVEMKQRPPVAVIPLGTGNDLARC
LRWGGGYEGESIPKLLEKISRSEAVMMDRWNVSIEATGDKEETIIPSHKVLKVTLSENVQ
RVVELSQRIVVEKSVINQNEQVQKTSEISIENSKRVILSDNNSSSTSTTFEKSREKTLES
NGMLQNSERICDKECTVERKSKGGEENCCNDAERNNNNNNINDNEAIKKPKSSTSCNDLL
MFGKQHSHREEIVPTTAADFSIPYNIINNYFSVGVDAAICVKFHLEREKNPHKFNSRMKN
KLWYFEYATSETFSATCKNLHEYVELTCDGIQLDLSGIQLQGIALLNIPYTHGGSNLWGE
NLSQKRLRRSTSSNGPFKKIKKLRSADKEMSTQSFNSVDLSIAIQDIGDRKIEVIGLESC
LHMGQVRTGLRASGRRLAQCSEIVLKTKKTFPMQIDGEPWMQEPATIKITHKNQVPMLAS
PKADKGRGFFNFNFFRR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 21 | g8316.t1 | CDD | cd00051 | EFh | 133 | 202 | 1.60146E-13 |
| 19 | g8316.t1 | CDD | cd00029 | C1 | 225 | 274 | 5.11912E-16 |
| 20 | g8316.t1 | CDD | cd00029 | C1 | 289 | 334 | 7.34461E-9 |
| 14 | g8316.t1 | Gene3D | G3DSA:1.10.238.110 | Diacylglycerol kinase alpha. | 7 | 134 | 2.1E-36 |
| 18 | g8316.t1 | Gene3D | G3DSA:1.10.238.10 | - | 135 | 206 | 2.3E-13 |
| 17 | g8316.t1 | Gene3D | G3DSA:3.30.60.20 | - | 218 | 287 | 2.3E-14 |
| 16 | g8316.t1 | Gene3D | G3DSA:3.30.60.20 | - | 288 | 335 | 1.6E-6 |
| 15 | g8316.t1 | Gene3D | G3DSA:3.40.50.10330 | - | 383 | 508 | 2.3E-14 |
| 31 | g8316.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 581 | 601 | - |
| 7 | g8316.t1 | PANTHER | PTHR11255 | DIACYLGLYCEROL KINASE | 93 | 912 | 4.0E-299 |
| 8 | g8316.t1 | PANTHER | PTHR11255:SF48 | DIACYLGLYCEROL KINASE 1 | 93 | 912 | 4.0E-299 |
| 3 | g8316.t1 | Pfam | PF14513 | Diacylglycerol kinase N-terminus | 10 | 92 | 1.9E-22 |
| 4 | g8316.t1 | Pfam | PF13202 | EF hand | 179 | 201 | 8.5E-4 |
| 5 | g8316.t1 | Pfam | PF00130 | Phorbol esters/diacylglycerol binding domain (C1 domain) | 225 | 275 | 2.2E-12 |
| 6 | g8316.t1 | Pfam | PF00130 | Phorbol esters/diacylglycerol binding domain (C1 domain) | 289 | 335 | 1.7E-9 |
| 2 | g8316.t1 | Pfam | PF00781 | Diacylglycerol kinase catalytic domain | 386 | 503 | 3.9E-29 |
| 1 | g8316.t1 | Pfam | PF00609 | Diacylglycerol kinase accessory domain | 687 | 879 | 9.2E-50 |
| 22 | g8316.t1 | ProSitePatterns | PS00018 | EF-hand calcium-binding domain. | 187 | 199 | - |
| 23 | g8316.t1 | ProSitePatterns | PS00479 | Zinc finger phorbol-ester/DAG-type signature. | 225 | 274 | - |
| 24 | g8316.t1 | ProSitePatterns | PS00479 | Zinc finger phorbol-ester/DAG-type signature. | 289 | 334 | - |
| 32 | g8316.t1 | ProSiteProfiles | PS50222 | EF-hand calcium-binding domain profile. | 129 | 164 | 11.11 |
| 33 | g8316.t1 | ProSiteProfiles | PS50222 | EF-hand calcium-binding domain profile. | 174 | 209 | 8.544 |
| 35 | g8316.t1 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 224 | 274 | 14.072 |
| 34 | g8316.t1 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 288 | 334 | 10.891 |
| 36 | g8316.t1 | ProSiteProfiles | PS50146 | DAG-kinase catalytic (DAGKc) domain profile. | 382 | 516 | 46.766 |
| 25 | g8316.t1 | SMART | SM00054 | efh_1 | 133 | 161 | 2.4 |
| 26 | g8316.t1 | SMART | SM00054 | efh_1 | 178 | 206 | 0.083 |
| 28 | g8316.t1 | SMART | SM00109 | c1_12 | 225 | 274 | 2.8E-12 |
| 29 | g8316.t1 | SMART | SM00109 | c1_12 | 289 | 334 | 1.9E-5 |
| 30 | g8316.t1 | SMART | SM00046 | dagk_c4a_7 | 386 | 510 | 8.0E-66 |
| 27 | g8316.t1 | SMART | SM00045 | dagk_c4b_2 | 686 | 879 | 4.5E-102 |
| 12 | g8316.t1 | SUPERFAMILY | SSF47473 | EF-hand | 9 | 137 | 3.77E-35 |
| 11 | g8316.t1 | SUPERFAMILY | SSF47473 | EF-hand | 49 | 211 | 3.53E-17 |
| 10 | g8316.t1 | SUPERFAMILY | SSF57889 | Cysteine-rich domain | 214 | 276 | 2.79E-15 |
| 9 | g8316.t1 | SUPERFAMILY | SSF57889 | Cysteine-rich domain | 282 | 338 | 1.42E-10 |
| 13 | g8316.t1 | SUPERFAMILY | SSF111331 | NAD kinase/diacylglycerol kinase-like | 386 | 896 | 2.4E-53 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004143 | diacylglycerol kinase activity | MF |
| GO:0007205 | protein kinase C-activating G protein-coupled receptor signaling pathway | BP |
| GO:0003951 | NAD+ kinase activity | MF |
| GO:0007165 | signal transduction | BP |
| GO:0016301 | kinase activity | MF |
| GO:0005509 | calcium ion binding | MF |
| GO:0035556 | intracellular signal transduction | BP |
KEGG
Orthology
- This transcript has been annotated with K00901 (diacylglycerol kinase (ATP))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed