General information for g8387.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase.
Parent gene
- The parent gene g8387 is located on chr_2: 30133846..30134620 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8387 | g8387.t1 | TTS | g8387.t1 | 30132465 | 30132465 |
| chr_2 | g8387 | g8387.t1 | isoform | g8387.t1 | 30133052 | 30134620 |
| chr_2 | g8387 | g8387.t1 | exon | g8387.t1.exon1 | 30133052 | 30134177 |
| chr_2 | g8387 | g8387.t1 | cds | g8387.t1.CDS1 | 30133052 | 30134177 |
| chr_2 | g8387 | g8387.t1 | exon | g8387.t1.exon2 | 30134240 | 30134512 |
| chr_2 | g8387 | g8387.t1 | cds | g8387.t1.CDS2 | 30134240 | 30134512 |
| chr_2 | g8387 | g8387.t1 | exon | g8387.t1.exon3 | 30134565 | 30134620 |
| chr_2 | g8387 | g8387.t1 | cds | g8387.t1.CDS3 | 30134565 | 30134620 |
| chr_2 | g8387 | g8387.t1 | TSS | g8387.t1 | 30134651 | 30134651 |
Sequences
>g8387.t1 Gene=g8387 Length=1455
ATGTTTTATCAATTTCTTGCATTTTCTTTTCTTAAATTAATTTTTATACCATCATATCGC
TCAACAGATTTCGAGGTTCATAGAAATTGGCTAGCGATTACACATTCATTAAATATTTCA
AGTTGGTATTATGAAGATACATCTGAATGGACATTAGATTATCCACCAATGTTTGCATAT
TTTGAGTATGCACTGTCACACATTGCAAAGTACTTTGATCCAGAAATGCTCATTGTGAGC
AACTTGAATTATGCTTCATACAACACAATTTTATTTCAAAGATTATCAGTAATATTTACT
GACGTTATCTATTTTCTCGGTGTTCGAGAATGTTTAAAAAATGTGAATAAAAATCAAAAG
GTTTTTCATTCAATTTTACTGCTTGGAAATATTGGATTAATTTTTGTGGATCACATTCAT
TTTCAATACAATGGAATATTATTTGGAATTCTTCTGCTTAGTTGTGGTAAAATTTTTCAA
CGAAAATATCTTCAAGCTGCATTTTACTACACATTATTGCTTAATATGAAGCATATTTAC
ATCTACATTGCTCCAGTTTATTTCTTTTATCTCCTCAAATTCTACTGTAGTGGATCATTT
AAACAATTCATTAGTGGAGTTATAAAATTAGGTACAATAGTACTACTCGTAACAGCAGCA
TCATTTGGACCATTTATTCATCATTTACCGCAAGTTTTGTCAAGACTGTTTCCATTTAAA
AGAGGACTAACACATGCCTATTGGGCACCAAATTTTTGGGCATTATACAACTTTGCAGAT
AAAATAATTTCAATTGTTAAAAAAATTCCGCAAAGTCATTCTAACACAGGAGGCTTAGTG
CAAGAATTTCAACATACTTATTTACCTACAATTTCTCCACAAGTGACATTCATTCTCACA
GCAGCCGCAATGCTTCCATGCTGCATTAAAATTTTATTTATCAAAAATGAGTCTATTAAG
AGTTTTATGAAACCATTGATAATTTGTGCAGCCACATCTTTTATGTTTGGATGGCATGTG
CATGAAAAAGCGATTTTAATGATTTTGATTCCTCTACAAGTGCTAACAGCTTATTTTCAA
TCAGAAGCAAATTCTTCTTTATTTTTAACAATTTTTGGTCTTTATTCTTTAACTCCATTG
TTATTTACAAGTGAATTAATTCTTGTTAAATTATCACTTTATGCACTTTATATTGCATTT
AATTTTTATGCATTTAGAAGAATTTACAAAGGACAGCAATTGTTAAAAATACATGAAAAT
TTGTATAGTTTAGGAATAATTTTATTACCACTTTATGAGTTTGTTATTCAATACATTTTA
AAATTAGATCAGAAATTTCCTTTTATGCCACTTATGTTAACAAGTGTTTATTGTGGTTTA
GGAATGATCTATTTTTGGCTCAAATTCTATTTTGAGTATTTACTAGAAACAAATGTAAAG
AAAAAGACTAAATAA
>g8387.t1 Gene=g8387 Length=484
MFYQFLAFSFLKLIFIPSYRSTDFEVHRNWLAITHSLNISSWYYEDTSEWTLDYPPMFAY
FEYALSHIAKYFDPEMLIVSNLNYASYNTILFQRLSVIFTDVIYFLGVRECLKNVNKNQK
VFHSILLLGNIGLIFVDHIHFQYNGILFGILLLSCGKIFQRKYLQAAFYYTLLLNMKHIY
IYIAPVYFFYLLKFYCSGSFKQFISGVIKLGTIVLLVTAASFGPFIHHLPQVLSRLFPFK
RGLTHAYWAPNFWALYNFADKIISIVKKIPQSHSNTGGLVQEFQHTYLPTISPQVTFILT
AAAMLPCCIKILFIKNESIKSFMKPLIICAATSFMFGWHVHEKAILMILIPLQVLTAYFQ
SEANSSLFLTIFGLYSLTPLLFTSELILVKLSLYALYIAFNFYAFRRIYKGQQLLKIHEN
LYSLGIILLPLYEFVIQYILKLDQKFPFMPLMLTSVYCGLGMIYFWLKFYFEYLLETNVK
KKTK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g8387.t1 | PANTHER | PTHR12413:SF2 | DOLICHYL PYROPHOSPHATE GLC1MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE-RELATED | 4 | 471 | 5.9E-166 |
| 3 | g8387.t1 | PANTHER | PTHR12413 | DOLICHYL GLYCOSYLTRANSFERASE | 4 | 471 | 5.9E-166 |
| 1 | g8387.t1 | Pfam | PF03155 | ALG6, ALG8 glycosyltransferase family | 10 | 467 | 6.6E-157 |
| 26 | g8387.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 27 | g8387.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 28 | g8387.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 16 | - |
| 40 | g8387.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 20 | - |
| 20 | g8387.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 89 | - |
| 32 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 90 | 109 | - |
| 17 | g8387.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 110 | 120 | - |
| 31 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 121 | 141 | - |
| 23 | g8387.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 142 | 178 | - |
| 30 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 179 | 196 | - |
| 19 | g8387.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 197 | 202 | - |
| 35 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 203 | 226 | - |
| 22 | g8387.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 227 | 294 | - |
| 34 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 295 | 314 | - |
| 15 | g8387.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 315 | 320 | - |
| 36 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 321 | 338 | - |
| 21 | g8387.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 339 | 343 | - |
| 38 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 344 | 360 | - |
| 18 | g8387.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 361 | 366 | - |
| 39 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 367 | 387 | - |
| 25 | g8387.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 388 | 392 | - |
| 29 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 393 | 409 | - |
| 16 | g8387.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 410 | 420 | - |
| 37 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 421 | 440 | - |
| 24 | g8387.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 441 | 445 | - |
| 33 | g8387.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 446 | 467 | - |
| 14 | g8387.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 468 | 484 | - |
| 13 | g8387.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
| 8 | g8387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 90 | 108 | - |
| 12 | g8387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 121 | 143 | - |
| 10 | g8387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 169 | 191 | - |
| 6 | g8387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 204 | 226 | - |
| 11 | g8387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 295 | 314 | - |
| 9 | g8387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 335 | 357 | - |
| 4 | g8387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 377 | 399 | - |
| 5 | g8387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 420 | 439 | - |
| 7 | g8387.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 449 | 471 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006490 | oligosaccharide-lipid intermediate biosynthetic process | BP |
| GO:0005783 | endoplasmic reticulum | CC |
| GO:0042283 | dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity | MF |
| GO:0016758 | hexosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K03849 (alpha-1,3-glucosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
