Gene loci information

Transcript annotation

  • This transcript has been annotated as Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g8387 g8387.t2 TTS g8387.t2 30132465 30132465
chr_2 g8387 g8387.t2 isoform g8387.t2 30133052 30134168
chr_2 g8387 g8387.t2 exon g8387.t2.exon1 30133052 30134168
chr_2 g8387 g8387.t2 cds g8387.t2.CDS1 30133052 30133981
chr_2 g8387 g8387.t2 TSS g8387.t2 30134651 30134651

Sequences

>g8387.t2 Gene=g8387 Length=1117
AAATGTGAATAAAAATCAAAAGGTTTTTCATTCAATTTTACTGCTTGGAAATATTGGATT
AATTTTTGTGGATCACATTCATTTTCAATACAATGGAATATTATTTGGAATTCTTCTGCT
TAGTTGTGGTAAAATTTTTCAACGAAAATATCTTCAAGCTGCATTTTACTACACATTATT
GCTTAATATGAAGCATATTTACATCTACATTGCTCCAGTTTATTTCTTTTATCTCCTCAA
ATTCTACTGTAGTGGATCATTTAAACAATTCATTAGTGGAGTTATAAAATTAGGTACAAT
AGTACTACTCGTAACAGCAGCATCATTTGGACCATTTATTCATCATTTACCGCAAGTTTT
GTCAAGACTGTTTCCATTTAAAAGAGGACTAACACATGCCTATTGGGCACCAAATTTTTG
GGCATTATACAACTTTGCAGATAAAATAATTTCAATTGTTAAAAAAATTCCGCAAAGTCA
TTCTAACACAGGAGGCTTAGTGCAAGAATTTCAACATACTTATTTACCTACAATTTCTCC
ACAAGTGACATTCATTCTCACAGCAGCCGCAATGCTTCCATGCTGCATTAAAATTTTATT
TATCAAAAATGAGTCTATTAAGAGTTTTATGAAACCATTGATAATTTGTGCAGCCACATC
TTTTATGTTTGGATGGCATGTGCATGAAAAAGCGATTTTAATGATTTTGATTCCTCTACA
AGTGCTAACAGCTTATTTTCAATCAGAAGCAAATTCTTCTTTATTTTTAACAATTTTTGG
TCTTTATTCTTTAACTCCATTGTTATTTACAAGTGAATTAATTCTTGTTAAATTATCACT
TTATGCACTTTATATTGCATTTAATTTTTATGCATTTAGAAGAATTTACAAAGGACAGCA
ATTGTTAAAAATACATGAAAATTTGTATAGTTTAGGAATAATTTTATTACCACTTTATGA
GTTTGTTATTCAATACATTTTAAAATTAGATCAGAAATTTCCTTTTATGCCACTTATGTT
AACAAGTGTTTATTGTGGTTTAGGAATGATCTATTTTTGGCTCAAATTCTATTTTGAGTA
TTTACTAGAAACAAATGTAAAGAAAAAGACTAAATAA

>g8387.t2 Gene=g8387 Length=309
MKHIYIYIAPVYFFYLLKFYCSGSFKQFISGVIKLGTIVLLVTAASFGPFIHHLPQVLSR
LFPFKRGLTHAYWAPNFWALYNFADKIISIVKKIPQSHSNTGGLVQEFQHTYLPTISPQV
TFILTAAAMLPCCIKILFIKNESIKSFMKPLIICAATSFMFGWHVHEKAILMILIPLQVL
TAYFQSEANSSLFLTIFGLYSLTPLLFTSELILVKLSLYALYIAFNFYAFRRIYKGQQLL
KIHENLYSLGIILLPLYEFVIQYILKLDQKFPFMPLMLTSVYCGLGMIYFWLKFYFEYLL
ETNVKKKTK

Protein features from InterProScan

Transcript Database ID Name Start End E.value
2 g8387.t2 PANTHER PTHR12413:SF2 DOLICHYL PYROPHOSPHATE GLC1MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE-RELATED 1 296 1.5E-87
3 g8387.t2 PANTHER PTHR12413 DOLICHYL GLYCOSYLTRANSFERASE 1 296 1.5E-87
1 g8387.t2 Pfam PF03155 ALG6, ALG8 glycosyltransferase family 1 292 5.3E-84
18 g8387.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 5 -
21 g8387.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 6 25 -
12 g8387.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 26 31 -
22 g8387.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 32 51 -
20 g8387.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 52 119 -
23 g8387.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 120 139 -
11 g8387.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 140 145 -
28 g8387.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 146 163 -
17 g8387.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 164 168 -
24 g8387.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 169 185 -
13 g8387.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 186 191 -
25 g8387.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 192 212 -
16 g8387.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 213 217 -
29 g8387.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 218 234 -
14 g8387.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 235 245 -
27 g8387.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 246 265 -
19 g8387.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 266 270 -
26 g8387.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 271 292 -
15 g8387.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 293 309 -
5 g8387.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 4 21 -
7 g8387.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 28 50 -
9 g8387.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 120 139 -
6 g8387.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 160 182 -
10 g8387.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 202 224 -
4 g8387.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 245 264 -
8 g8387.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 274 296 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006490 oligosaccharide-lipid intermediate biosynthetic process BP
GO:0005783 endoplasmic reticulum CC
GO:0042283 dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity MF
GO:0016758 hexosyltransferase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values