General information for g8387.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase.
Parent gene
- The parent gene g8387 is located on chr_2: 30133846..30134620 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8387 | g8387.t4 | TTS | g8387.t4 | 30133034 | 30133034 |
| chr_2 | g8387 | g8387.t4 | isoform | g8387.t4 | 30133846 | 30134620 |
| chr_2 | g8387 | g8387.t4 | exon | g8387.t4.exon1 | 30133846 | 30134177 |
| chr_2 | g8387 | g8387.t4 | exon | g8387.t4.exon2 | 30134236 | 30134512 |
| chr_2 | g8387 | g8387.t4 | cds | g8387.t4.CDS1 | 30134236 | 30134512 |
| chr_2 | g8387 | g8387.t4 | exon | g8387.t4.exon3 | 30134565 | 30134620 |
| chr_2 | g8387 | g8387.t4 | cds | g8387.t4.CDS2 | 30134565 | 30134620 |
| chr_2 | g8387 | g8387.t4 | TSS | g8387.t4 | 30134651 | 30134651 |
Sequences
>g8387.t4 Gene=g8387 Length=665
ATGTTTTATCAATTTCTTGCATTTTCTTTTCTTAAATTAATTTTTATACCATCATATCGC
TCAACAGATTTCGAGGTTCATAGAAATTGGCTAGCGATTACACATTCATTAAATATTTCA
AGTTGGTATTATGAAGATACATCTGAATGGACATTAGATTATCCACCAATGTTTGCATAT
TTTGAGTATGCACTGTCACACATTGCAAAGTACTTTGATCCAGAAATGCTCATTGTGAGC
AACTTGAATTATGCTTCATACAACACAATTTTATTTCAAAGATTATCAGTAATATTTACT
GACGTTATCTATTTTCTCGGTGTTCGAGAGTGAATGTTTAAAAAATGTGAATAAAAATCA
AAAGGTTTTTCATTCAATTTTACTGCTTGGAAATATTGGATTAATTTTTGTGGATCACAT
TCATTTTCAATACAATGGAATATTATTTGGAATTCTTCTGCTTAGTTGTGGTAAAATTTT
TCAACGAAAATATCTTCAAGCTGCATTTTACTACACATTATTGCTTAATATGAAGCATAT
TTACATCTACATTGCTCCAGTTTATTTCTTTTATCTCCTCAAATTCTACTGTAGTGGATC
ATTTAAACAATTCATTAGTGGAGTTATAAAATTAGGTACAATAGTACTACTCGTAACAGC
AGCAT
>g8387.t4 Gene=g8387 Length=110
MFYQFLAFSFLKLIFIPSYRSTDFEVHRNWLAITHSLNISSWYYEDTSEWTLDYPPMFAY
FEYALSHIAKYFDPEMLIVSNLNYASYNTILFQRLSVIFTDVIYFLGVRE
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g8387.t4 | PANTHER | PTHR12413:SF2 | DOLICHYL PYROPHOSPHATE GLC1MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE-RELATED | 4 | 109 | 3.9E-47 |
| 3 | g8387.t4 | PANTHER | PTHR12413 | DOLICHYL GLYCOSYLTRANSFERASE | 4 | 109 | 3.9E-47 |
| 1 | g8387.t4 | Pfam | PF03155 | ALG6, ALG8 glycosyltransferase family | 10 | 107 | 3.6E-45 |
| 6 | g8387.t4 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 7 | g8387.t4 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 8 | g8387.t4 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 16 | - |
| 9 | g8387.t4 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 20 | - |
| 5 | g8387.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 110 | - |
| 4 | g8387.t4 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006490 | oligosaccharide-lipid intermediate biosynthetic process | BP |
| GO:0005783 | endoplasmic reticulum | CC |
| GO:0042283 | dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase activity | MF |
| GO:0016758 | hexosyltransferase activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
