General information for g8463.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Serine/threonine-protein kinase PAK mbt.
Parent gene
- The parent gene g8463 is located on chr_2: 30843435..30843991 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8463 | g8463.t2 | isoform | g8463.t2 | 30841506 | 30842271 |
| chr_2 | g8463 | g8463.t2 | exon | g8463.t2.exon1 | 30841506 | 30842077 |
| chr_2 | g8463 | g8463.t2 | TTS | g8463.t2 | 30841520 | 30841520 |
| chr_2 | g8463 | g8463.t2 | cds | g8463.t2.CDS1 | 30841877 | 30842077 |
| chr_2 | g8463 | g8463.t2 | exon | g8463.t2.exon2 | 30842148 | 30842271 |
| chr_2 | g8463 | g8463.t2 | cds | g8463.t2.CDS2 | 30842148 | 30842270 |
| chr_2 | g8463 | g8463.t2 | TSS | g8463.t2 | NA | NA |
Sequences
>g8463.t2 Gene=g8463 Length=696
GATGTCACCAGAAGTTATTTCACGACTTCTTTATGGACCGGAAGTTGATATTTGGTCACT
TGGTATTATGGTAATTGAAATGATTGACGCGGAGCCACCATTTTTCAATGAACCACCTTT
ACAAGCAATGAGAAGAATTCGAGATATGCCACCACCAACACCGAAAAATGCACACAAAGT
TTCACCTCGATTGCGTCGTTTTCTCGATCGAATGCTTGTAAGAGATCCAGCACAACGAGC
TACAGCTGCAGAGTTGTTATCCGATCCATTCCTACTTGTCGCTGGACCACCTTCAATTTT
AGTCCCAAGAATGAAAGGTTTCTAATAATCGACTTAATTTTTATATATTTAAAGATATAT
TTGATACTGTAATCAAAACTCGAATGTATCAACTGACAATTTTTTTTTTTGCGTTTTTAT
TATTCAATCAGCTGTAAGTGAAAATCTACTTATCAGTACTTATTTTTTCTTTTTTATATT
CGACAACAAAAGTATTAAAAAAATAATAATACAATGAAATTAAAAAAAACAATCCAATTT
CCTTCTTCCTTCTCAATTTCCATTCCATTCCCTTAATTTTTTATTAAATAATATTTATCA
TTGTTGTAAGTTACACACATATACACATACAGTGCTAAAACAAAAAGAATTGAAGAATAT
TTCAATAAAGACTTTTTTATATAAAAAAAAACTTTT
>g8463.t2 Gene=g8463 Length=107
MSPEVISRLLYGPEVDIWSLGIMVIEMIDAEPPFFNEPPLQAMRRIRDMPPPTPKNAHKV
SPRLRRFLDRMLVRDPAQRATAAELLSDPFLLVAGPPSILVPRMKGF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g8463.t2 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1 | 107 | 0.000 |
| 2 | g8463.t2 | PANTHER | PTHR48015:SF27 | SERINE/THREONINE-PROTEIN KINASE PAK 5 | 1 | 106 | 0.000 |
| 3 | g8463.t2 | PANTHER | PTHR48015 | SERINE/THREONINE-PROTEIN KINASE TAO | 1 | 106 | 0.000 |
| 1 | g8463.t2 | Pfam | PF00069 | Protein kinase domain | 1 | 91 | 0.000 |
| 6 | g8463.t2 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1 | 91 | 17.687 |
| 4 | g8463.t2 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 1 | 94 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
