General information for g8464.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as UDP-glucose 6-dehydrogenase.
Parent gene
- The parent gene g8464 is located on chr_2: 30845876..30847199 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8464 | g8464.t2 | TTS | g8464.t2 | 30844654 | 30844654 |
| chr_2 | g8464 | g8464.t2 | isoform | g8464.t2 | 30844813 | 30845977 |
| chr_2 | g8464 | g8464.t2 | exon | g8464.t2.exon1 | 30844813 | 30845215 |
| chr_2 | g8464 | g8464.t2 | cds | g8464.t2.CDS1 | 30844813 | 30845215 |
| chr_2 | g8464 | g8464.t2 | exon | g8464.t2.exon2 | 30845272 | 30845977 |
| chr_2 | g8464 | g8464.t2 | cds | g8464.t2.CDS2 | 30845272 | 30845888 |
| chr_2 | g8464 | g8464.t2 | TSS | g8464.t2 | NA | NA |
Sequences
>g8464.t2 Gene=g8464 Length=1109
GTTGCGCTCGATTGATAGCAAATATCGCACAGAGTAGTAAAATTGTCGTTGAGAAAAGTA
CAGTGCCAGTGCGAGCAGCTGAAAGTATTATGCATGTATTGAAGGCCAATAATAAGCCAG
GAGTAAAATATTCAATTCTTTCGAATCCTGAATTCCTTGCTGAAGGAACTGCCATTGAAA
ATCTAATGAAACCTGATCGAGTTTTAATTGGAGGTGAAGAAACTTTAGATGGAAAAAAAG
CGGTTGAAACCTTATGTTGGGTTTATGAGCATTGGGTATCAAAAGATAAAATTTTAACAA
CAAACACTTGGAGTTCAGAACTTTCAAAACTTGCTGCCAATGCAATGCTCGCTCAACGAA
TTAGCAGTATTAATTCACTCTCAGCTGTTTGTGAAAGCACTGGTGCTGATGTATCAGAAG
TTGCTCGTGCAATTGGCTTAGATTCAAGAATCGGCTCGAAATTTTTACAAGCTTCAGTTG
GATTCGGTGGAAGTTGCTTTCAAAAAGATCTTTTGAATTTGGTTTATATTTGTGAGAAAT
TGAATCTACCAGAAGTTGCTCAATATTGGCAGAAAGTCATTGATATGAATGAATATCAAA
AGGAAAGATTTGCACACAAAATTATTGAGTGTCTTTTCAATACTGTCAATGAAAAAAGAA
TTTCAGTTCTTGGATTTGCTTTTAAAAAGAACACTGGTGATACTCGTGAAACTCCAGCAA
TTTCTGTCTGCAAAGTTCTCCTTGATGAAGGCGCAAAATTAAATATTTATGATCCGAAAG
TAGAGAAATCTCAAATTATTAATGACTTAACATCTCCACGTGTTACTGATTTTCCCGATG
AGACGAGAAAATCAATTGAAATTTTCTCAGATCCATATGCAGCTGTGGAAAACACACATG
CGATTGTTATATGCACTGAATGGGATGAATTTGTTGAATTGGATTATAATCGAATCTTAC
AATCAATGATGAAGCCAGCTTACATTTTTGATGGTCGAAAAATATTGAATCATGAGGAGT
TGCAGGATCTCGGCTTTCATGTCATTACAATTGGCAAAAAACTTAATAGAATTGGTTTTA
GAACTTTTGGTAGTATTCCACAACAATGA
>g8464.t2 Gene=g8464 Length=339
MHVLKANNKPGVKYSILSNPEFLAEGTAIENLMKPDRVLIGGEETLDGKKAVETLCWVYE
HWVSKDKILTTNTWSSELSKLAANAMLAQRISSINSLSAVCESTGADVSEVARAIGLDSR
IGSKFLQASVGFGGSCFQKDLLNLVYICEKLNLPEVAQYWQKVIDMNEYQKERFAHKIIE
CLFNTVNEKRISVLGFAFKKNTGDTRETPAISVCKVLLDEGAKLNIYDPKVEKSQIINDL
TSPRVTDFPDETRKSIEIFSDPYAAVENTHAIVICTEWDEFVELDYNRILQSMMKPAYIF
DGRKILNHEELQDLGFHVITIGKKLNRIGFRTFGSIPQQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g8464.t2 | Gene3D | G3DSA:3.40.50.720 | - | 1 | 72 | 0.0e+00 |
| 12 | g8464.t2 | Gene3D | G3DSA:1.20.5.100 | - | 73 | 104 | 0.0e+00 |
| 11 | g8464.t2 | Gene3D | G3DSA:3.40.50.720 | - | 105 | 332 | 0.0e+00 |
| 4 | g8464.t2 | PANTHER | PTHR11374 | UDP-GLUCOSE DEHYDROGENASE/UDP-MANNAC DEHYDROGENASE | 3 | 331 | 0.0e+00 |
| 5 | g8464.t2 | PANTHER | PTHR11374:SF34 | UDP-GLUCOSE 6-DEHYDROGENASE | 3 | 331 | 0.0e+00 |
| 14 | g8464.t2 | PIRSF | PIRSF500134 | UDPglc_DH_bac | 1 | 326 | 0.0e+00 |
| 15 | g8464.t2 | PIRSF | PIRSF000124 | UDPglc_GDPman_dh | 2 | 323 | 0.0e+00 |
| 2 | g8464.t2 | Pfam | PF03721 | UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain | 5 | 48 | 0.0e+00 |
| 3 | g8464.t2 | Pfam | PF00984 | UDP-glucose/GDP-mannose dehydrogenase family, central domain | 74 | 168 | 0.0e+00 |
| 1 | g8464.t2 | Pfam | PF03720 | UDP-glucose/GDP-mannose dehydrogenase family, UDP binding domain | 193 | 307 | 0.0e+00 |
| 9 | g8464.t2 | SMART | SM00984 | UDPG_MGDP_dh_C_a_2_a | 192 | 308 | 0.0e+00 |
| 6 | g8464.t2 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 13 | 78 | 3.1e-06 |
| 7 | g8464.t2 | SUPERFAMILY | SSF48179 | 6-phosphogluconate dehydrogenase C-terminal domain-like | 74 | 173 | 0.0e+00 |
| 8 | g8464.t2 | SUPERFAMILY | SSF52413 | UDP-glucose/GDP-mannose dehydrogenase C-terminal domain | 177 | 317 | 0.0e+00 |
| 13 | g8464.t2 | TIGRFAM | TIGR03026 | NDP-sugDHase: nucleotide sugar dehydrogenase | 7 | 304 | 0.0e+00 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016616 | oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor | MF |
| GO:0003979 | UDP-glucose 6-dehydrogenase activity | MF |
| GO:0051287 | NAD binding | MF |
| GO:0000271 | polysaccharide biosynthetic process | BP |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
