General information for g8480.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Zinc transporter foi.
Parent gene
- The parent gene g8480 is located on chr_2: 30908350..30909470 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g8480 | g8480.t4 | TSS | g8480.t4 | 30904729 | 30904729 |
| chr_2 | g8480 | g8480.t4 | isoform | g8480.t4 | 30904836 | 30907891 |
| chr_2 | g8480 | g8480.t4 | exon | g8480.t4.exon1 | 30904836 | 30905563 |
| chr_2 | g8480 | g8480.t4 | exon | g8480.t4.exon2 | 30906395 | 30907891 |
| chr_2 | g8480 | g8480.t4 | cds | g8480.t4.CDS1 | 30906423 | 30907889 |
| chr_2 | g8480 | g8480.t4 | TTS | g8480.t4 | 30906620 | 30906620 |
Sequences
>g8480.t4 Gene=g8480 Length=2225
TTGTCAGTTTGAAAATTGAACATTTTAAATTTTTAATCAATATTGACAATTCTTTATTTG
ATTTTGTGTTTGAGACCCTAACATGACGCAATTACATAAAAGTGCGTTAGTATTCGTAAG
CGGCTTTTCTCTCTCTCAAATTTTGTAGCGTGGTTTCGAAATCACGACCACTGCTAATTA
TTTGATACTTGTAAACTTTATCAAAAAAGCTCCATTAAACCTTTGCCATGCATAACGTTT
CAATTTTATTTTTTTCATCGATTCTCTTTTTCATTTAGTTCTGCCCAATTTTGGTTGTTC
AATTTTTTTCAATTGACAGATTTATTGGCATTGGTCAGTCGATAATTTGGTCACTATGAA
GAATGCTTAAAAGTGTAATTTAAAGATTCAAGATTATAGATAAGCACACTTTAATCAAAA
CGTAAACATTTCTATCAAATCAAAAAGCAGGAAAAAAATTGAATGGAGAAAAAAAATCTA
TTTGCGATCTATCATTCTTTAAGTGTACTTTCAACAGTGAACACGTTTATTTAGTAGCGA
GAGAATATTCACGCTTTCTTTATACGGCGTGAACGTTGTTATATACCACCTACTACTTTA
ATGTGAAAATTAAAGTGTCAAAAAGAGCTTAGGTTATCGGACACAAATTGAGAAATCGTT
TATAAAGTCCAGTGATATAGAAACAAAATGTTTTTAAATTTAACCATGGCTTTAATTTTA
TCAATCAAATTTTCTTTTATATTTATAAAAAACACAATGGCGCATCGTCATTTAATGGCA
GTGTGTGTTGTATGCTTATTATGTGCTGAACATTTACCATGTAAGCACGTTTTAGCGGCA
TCAACAACGAATATTATTAAATCCAAGGATGAAAGATGGTCATTAGAAACATCAACTGAA
AATGATGCATTAGTATTACTTGCTGATGAAGTAGCAGTTGAAAATATTTATGACAAAAGA
AAAGGAAAAAATAAAGACGAAGAAAAAATGAGAGTAAAACGTCATGCTGATCATTCAAAT
AGTCATAAACACCATCATATTGAATTGCCAGAAGTAACAAAAAATTATCTACAAAAAATA
TTCCTTCAATTTAATGAACAACAACAGCAACAACAATCAATGCAAATGAACATGAATGGA
TTTGAGAATATGATAGGACAATTAAAATTGAATCATATATTTAATTCTTCTATTAATGAT
AAAATGTGTATGAATGAAGAGACTTTTCTAGATAAAATAACACATCATAATCACCACGAA
TTAGAGCATGAACATGAAGAAGAGGACCACGAACATGAACATCATGATCATCATATAGAA
ATTAGTACTGACAACATGCTTTCAATGTGTCCAATTTTACTTTACTATGCAATTAATCGC
GACATAAGTTCATGTTTGCATATTTCAAATTTTAGTATGTTAGATTTGAATGACGAAACG
TCATCGCTGATAGTTATGGAAGATAGAAAACTTGTGTGGCTCTATTCAACTTTAAGCATA
ATTCTTGTTAGCCTTTGTGGACTGTTTGGTGTTGCAGTGATTCCCATTATGGAAAAACAT
TTTTATCATCACATATTGCAATTTTTAGTGGCACTTGCTGTGGGCACACTTGCAGGCGAT
GCATTGCTTCATTTGCTTCCGCATGCTATGCTTCCGCTCACAAATGATCAAGACTTACAT
CAAACTATGATGTATCGAGGTTTGGCAGCAGTTTTAGGAATTGTATTTTTCTATTTCTTT
GAACGATTCATGGTGATGATAATGGAATGGCGACAAAAGAAAGAGAAACGTGAAAAACCT
TCATCACGTGTTCGTGTAATGCGTGATCCAGAAACTGTTTCCTTAAATGGTGCAAACATG
ACATGCAAACATAAATATTCCTCATACCCATATTGCTACGATGAAATAGCCATGGAAACA
AAGGACGATCATCATGAGCATCAACACATTGAGGAGGAAGCAGTTGTAAATAATCACAAA
GATCTCATCATAACATCCGCATCTGATATTAAAAAAATGAACGGATTAATTCATGATAAT
AGCAATGATGTTGATAATACTACACTGACATTATCTACAAGCTTGGATGATGGATCAATT
GAAAGCAATGCGCTTTGTAACAATAATTCTAATAAACAGACTGGAAATCCTATTTCAAAC
ACCACAACAAATTGTAAAACTACTGGAGAAAATCTACAAGAAGAAAATTATACAATTATT
TTGAG
>g8480.t4 Gene=g8480 Length=489
MAHRHLMAVCVVCLLCAEHLPCKHVLAASTTNIIKSKDERWSLETSTENDALVLLADEVA
VENIYDKRKGKNKDEEKMRVKRHADHSNSHKHHHIELPEVTKNYLQKIFLQFNEQQQQQQ
SMQMNMNGFENMIGQLKLNHIFNSSINDKMCMNEETFLDKITHHNHHELEHEHEEEDHEH
EHHDHHIEISTDNMLSMCPILLYYAINRDISSCLHISNFSMLDLNDETSSLIVMEDRKLV
WLYSTLSIILVSLCGLFGVAVIPIMEKHFYHHILQFLVALAVGTLAGDALLHLLPHAMLP
LTNDQDLHQTMMYRGLAAVLGIVFFYFFERFMVMIMEWRQKKEKREKPSSRVRVMRDPET
VSLNGANMTCKHKYSSYPYCYDEIAMETKDDHHEHQHIEEEAVVNNHKDLIITSASDIKK
MNGLIHDNSNDVDNTTLTLSTSLDDGSIESNALCNNNSNKQTGNPISNTTTNCKTTGENL
QEENYTIIL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g8480.t4 | Coils | Coil | Coil | 489 | 489 | - |
| 2 | g8480.t4 | PANTHER | PTHR12191 | SOLUTE CARRIER FAMILY 39 | 186 | 404 | 1.2E-46 |
| 3 | g8480.t4 | PANTHER | PTHR12191:SF30 | ZINC TRANSPORTER FOI | 186 | 404 | 1.2E-46 |
| 1 | g8480.t4 | Pfam | PF02535 | ZIP Zinc transporter | 239 | 403 | 1.5E-27 |
| 14 | g8480.t4 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 27 | - |
| 15 | g8480.t4 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 16 | g8480.t4 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 17 | - |
| 20 | g8480.t4 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 18 | 27 | - |
| 13 | g8480.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 28 | 239 | - |
| 18 | g8480.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 240 | 262 | - |
| 10 | g8480.t4 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 263 | 273 | - |
| 17 | g8480.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 274 | 295 | - |
| 12 | g8480.t4 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 296 | 314 | - |
| 19 | g8480.t4 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 315 | 335 | - |
| 11 | g8480.t4 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 336 | 489 | - |
| 8 | g8480.t4 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 27 | - |
| 7 | g8480.t4 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 27 | - |
| 5 | g8480.t4 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 240 | 262 | - |
| 4 | g8480.t4 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 269 | 291 | - |
| 6 | g8480.t4 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 311 | 333 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0055085 | transmembrane transport | BP |
| GO:0046873 | metal ion transmembrane transporter activity | MF |
| GO:0030001 | metal ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
